Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.1, No.5, Oct. 2015, Pub. Date: Jul. 9, 2015
Hepatotoxic Effect of Aflatoxin-Contaminated Agro Feeds (Groundnut, Maize & Melon Seed) on Wistar Albino Rats
Pages: 190-196 Views: 2036 Downloads: 1163
[01] Onyegeme-Okerenta Blessing M., Department of Biochemistry, Faculty of Chemical Sciences, College of Natural and Applied Sciences, University of Port Harcourt, Choba, Rivers State, Nigeria.
[02] Enyadike Nelson U., Department of Biochemistry, Faculty of Chemical Sciences, College of Natural and Applied Sciences, University of Port Harcourt, Choba, Rivers State, Nigeria.
The present study is an attempt to evaluate the hepatotoxicity of aflatoxin-contaminated feed on Wister albino rats. Thirty-six experimental albino rats were grouped into four (4); the control group (CT) was fed with uncontaminated commercial feed, group AG was fed with aflatoxin-contaminated groundnut, group BM was fed with aflatoxin-contaminated maize grain and group CM was fed with aflatoxin-contaminated melon seed. The aflatoxin concentrations of the various feeds were measured using Enzyme linked Immunosorbent Assay (ELISA) method. The rats were fed for a period of 21 days while three rats from each group were sacrificed weekly to study various liver enzyme activities - gamma glutamyl transferase [GGT], aspartate amino transferase [AST], alanine amino transferase [ALT] and lactate dehydrogenase [LDH]). Results of AST activity showed that AG (52.00±1.00U/L), BM (30.00±1.00U/L) and CM (46.66±0.57U/L) were significantly higher (p<0.05) when compared to CT (26.33 ± 0.57 U/L) after week 1 of feeding the rats. Similarly GGT, LDH, and ALT activities of groups fed with aflatoxin-contaminated feeds after weeks 1, 2 and 3 were significantly higher (p<0.05) when compared to control group. The results reveal that the aflatoxin present in the groups fed with aflatoxin–contaminated feeds is responsible for the increased enzyme activities observed. Histopathological analysis of photomicrographs showed varying degrees of hepatotoxicity of AG, BM and CM when compared to control group.
Aflatoxin, Hepatotoxicity, Contaminated Agro Feeds, Liver Enzyme Activity, Hepatocytes
[01] Abt Associates. (2012). Aflatoxin Contamination and Potential Solutions for Its Control in Nigeria: A summary of the country and economic assessment conducted in 2012 and the aflatoxin stakeholder workshop held on November 5 and 6, 2012 in Abuja.
[02] Adejumo, T.O. & Orole, O.O. (2015). Carcinogens in West Africa with Special Reference to Fungal Metabolites from Fusarium species. Journal of Biology, Agriculture and Healthcare, 5(8), pp. 21-46.
[03] Agag, B.I. (2004). Mycotoxins in foods and feeds 1-Aflatoxins. Assiut University Bulletin for Environmental Researches, Vol.7, No.1, pp.173-205.
[04] Ayejuyo, O.O., Olowu, R. A., Agbaje, T.O., Atamenwan, M. & Osundiya, M.O. (2011). Enzyme - Linked Immunosorbent Assay (ELISA) of Aflatoxin B1 in Groundnut and Cereal Grains in Lagos. Nigeria Research Journal of Chemical Sciences, 1(8), 1-5.
[05] Bandyopadhyay, R.P., Kumar, M. & Leslie, J.F. (2007) Relative Severity of Aflatoxin Contamination of Cereal Crops in West Africa. Food Additives and Contaminants, 24 (10), pp. 1109-1114.
[06] Brown, H.S. (2002). Hematoxylin and eosin (the routine stain). H&H informational primer. Sigma-Aldrich Corporation, pp 1–3.
[07] Clifford, J.I. & Rees, K.R. (1966). Action of Aflatoxin on Rat Liver. Biochemical Journal (1967) 102, 65 p 66-75.
[08] Devendran, G. & Balasubramanian, U. (2011) Biochemical and histopathological analysis of aflatoxin induced toxicity in liver and kidney of rat. Asian Journal of Plant Science and Research, 1 (4):61-69.
[09] Dhanasekaran, D., Shanmugapriya, S., Thajuddin, N. & Panneerselvam, A. (2011). Aflatoxins and Aflatoxicosis in Human and Animals: In Ramón Gerardo Guevara-González Aflatoxins - Biochemistry and Molecular Biology, InTech, Croatia.
[10] Diaz, G.J., Calabrese, E. & Blain, R., (2008). Aflatoxicosis in chickens (Gallus gallus): A Review of History and Contemporary Issues. Toxins, 3, 566-590.
[11] Fouzia, B. & Samajpati, N. (2000). Mycotoxins production on rice, pulses and oilseeds. Naturwissenschaften, Vol.87, pp.275-277.
[12] Kubena L. F., Harvey R. B., Bailey R. H., Buckley S. A. & Rottinghaus G. E. (1998). Effects of hydrated sodium calcium aluminosilicate (T-Bind™) on mycotoxicosis in young broiler chickens. Poultry Science, 77:1502–1509.
[13] Leeson, S., Diaz, G.J. & Summers, J.D. (1995). Poultry metabolic disorders and mycotixns. pp. 249-298, University Books, Guelph, Ontario, Canada
[14] Liu Y. & Wu F. (2010). Global Burden of Aflatoxin-Induced Hepatocellular Carcinoma: A Risk Assessment. Environmental Health Perspectives, 118(6), pp. 818-824.
[15] Marin, D.E., Taranu, I., Bunaciu, R.P., Pascale, F., Tudor, D.S., Avram, N., Sarca, M., Cureu, I., Criste, R.D., Suta, V. & Oswald, I.P., (2002). Changes in performance, blood parameters, humoral and cellular immune responses in weanling piglets exposed to low doses of aflatoxin. Journal of Animal Science, 80,1250-1257.
[16] Mushtaq, M., Sultana, B., Anwar, F., Khan, M.Z. & Ashrafuzzaman, M. (2012) Occurrence of aflatoxins in selected processed foods from Pakistan. International Journal of Molecular Sciences, 13, 8324-8337.
[17] Standards Organization of Nigeria (SON). (2003). “Standard for Maize Grit,” National Industrial Standard 253:2003,
[18] Standards Organization of Nigeria (SON). (2006). “Standard for Groundnut Seed,” National Industrial Standard 491:2006.
[19] Standards Organization of Nigeria (SON). (2008). “Standard for Kulikuli (Groundnut cake).” National Industrial Standard 594:2008.
[20] Strosnider, H., Azziz-Baumgartner, E., Banziger, M., Bhat, R.V., Breiman, R., Brune, M., DeCock, K., Dilley, A., Groopman, J., Hell, K., Henry, S.H., Jeffers, D., Jolly, C., Jolly, P., Kibata, G.N., Lewis, L., Liu, X., Luber, G., McCoy, L., Mensah, P., Miraglia, M., Misore, A., Njapau, H., Ong, C., Onsongo, M.T.K., Page, S.W., Park, D., Patel, M., Phillips, T., Pineiro, M., Pronczuk, J., Schurz Rogers, H., Rubin, C., Sabino, M., Schaafsma, A., Shephard, G., Stroka, J., Wild, C., Williams, J.T. & Wilson, D. (2006). “Workgroup Report: Public Health Strategies for Reducing Aflatoxin Exposure in Developing Countries.” Environmental Health Perspectives, 114:1989-1903.
[21] Tulayakul, P., Sakuda, S., Dong, K.S. & Kumagai, S. (2005). Comparative activities of glutathione-S-transferase and dialdehydereductase toward aflatoxin B1 in livers of experimental and farm animals. Toxicon, Vol.46, No.2, pp. 204-209.
[22] Yunus, A. W., Ghareeb, K., Abd-El-Fattah, A. A. M., Twaruzek, M. & Bohm, J. (2011). Gross intestinal adaptations in relation to broiler performance during a chronic aflatoxin exposure. Poultry Science, 90: 1683-1689.
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