Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.1, No.5, Oct. 2015, Pub. Date: Jul. 9, 2015
Hepatotoxic Effect of Aflatoxin-Contaminated Agro Feeds (Groundnut, Maize & Melon Seed) on Wistar Albino Rats
Pages: 190-196 Views: 2154 Downloads: 1281
[01] Onyegeme-Okerenta Blessing M., Department of Biochemistry, Faculty of Chemical Sciences, College of Natural and Applied Sciences, University of Port Harcourt, Choba, Rivers State, Nigeria.
[02] Enyadike Nelson U., Department of Biochemistry, Faculty of Chemical Sciences, College of Natural and Applied Sciences, University of Port Harcourt, Choba, Rivers State, Nigeria.
The present study is an attempt to evaluate the hepatotoxicity of aflatoxin-contaminated feed on Wister albino rats. Thirty-six experimental albino rats were grouped into four (4); the control group (CT) was fed with uncontaminated commercial feed, group AG was fed with aflatoxin-contaminated groundnut, group BM was fed with aflatoxin-contaminated maize grain and group CM was fed with aflatoxin-contaminated melon seed. The aflatoxin concentrations of the various feeds were measured using Enzyme linked Immunosorbent Assay (ELISA) method. The rats were fed for a period of 21 days while three rats from each group were sacrificed weekly to study various liver enzyme activities - gamma glutamyl transferase [GGT], aspartate amino transferase [AST], alanine amino transferase [ALT] and lactate dehydrogenase [LDH]). Results of AST activity showed that AG (52.00±1.00U/L), BM (30.00±1.00U/L) and CM (46.66±0.57U/L) were significantly higher (p<0.05) when compared to CT (26.33 ± 0.57 U/L) after week 1 of feeding the rats. Similarly GGT, LDH, and ALT activities of groups fed with aflatoxin-contaminated feeds after weeks 1, 2 and 3 were significantly higher (p<0.05) when compared to control group. The results reveal that the aflatoxin present in the groups fed with aflatoxin–contaminated feeds is responsible for the increased enzyme activities observed. Histopathological analysis of photomicrographs showed varying degrees of hepatotoxicity of AG, BM and CM when compared to control group.
Aflatoxin, Hepatotoxicity, Contaminated Agro Feeds, Liver Enzyme Activity, Hepatocytes
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