Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.1, No.2, Apr. 2015, Pub. Date: Mar. 21, 2015
Genetic Characterization of Lentil (Lens culinaris L.) Germplasm by Using SSR Markers
Pages: 16-26 Views: 2596 Downloads: 1624
Authors
[01] U. K. S. Kushwaha, Institute of Agriculture and Animal Science, Department of Plant Breeding, Tribhuvan University, Rampur, Chitwan, Nepal.
[02] S. K. Ghimire, Institute of Agriculture and Animal Science, Department of Plant Breeding, Tribhuvan University, Rampur, Chitwan, Nepal.
[03] N. K. Yadav, Nepal Agricultural Research Council, Singh Darbar, Kathmandu, Nepal.
[04] B. R. Ojha, Institute of Agriculture and Animal Science, Department of Plant Breeding, Tribhuvan University, Rampur, Chitwan, Nepal.
[05] R. K. Niroula, Nepal Agricultural Research Council, Biotechnology Unit, Khumaltar, Lalitpur.
Abstract
Ninety six lentil accessions were collected from National Grain Legume Research Program, Rampur; Regional Agriculture Research Station, Nepalgunj and National Agriculture Genetic Resource Center, Khumaltar, Lalitpur.Among them; four lines were Nepal Local, forty two lines were Nepal Cross; forty seven lines were ICARDA Line and finally three lines were Indian Line. Allaccessions were analysed by DNA fingerprinting using thirty three selected polymorphic SSR markers. The characterization was performed in Biotechnology Unit, Nepal Agricultural Research Council, Khumaltar, Lalitpurin 2012 by using standard protocols. The dendrogram constructed based on Jaccard’s similarity coefficient and UPGMA separated all accessions into four clusters with two major groups. Group A consisted of three cluster (I, II and III) whereas Group B comprised only one cluster (IV). Cluster I represented 58.33 % of total genotypes followed by cluster II (38.54%), cluster III (2.08 %) and cluster IV (1.04 %). Cluster I was the largest and cluster IV was the smallest cluster based on number of genotypes. Microsatellite profiling based dendrogram revealed that RL lines were more diverged than ILL lines. Among all lentil accessions Sagun and LN 0135 showed distant relationship as compared to others in dendrogram. The first three Eigen values of three principal coordinate axes explained 71.08 % genetic variation and the first two principal axes accounted for 53.76 % of total variation describing ninety six genotypes. The clustering pattern obtained coincided with the apparent grouping patterns performed by PCA. So the results obtained through PCA were confirmed by non-hierarchical clustering. All the accessions included in the study displayed significant amount of genetic variability due to different center of origin and different genetic constitutions. The diversity detected in this study may constitute the new materials for future systematic lentil breeding programs.
Keywords
Lentil, Genotypes, Diversity, Cluster, Marker
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