Agricultural and Biological Sciences Journal
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Agricultural and Biological Sciences Journal, Vol.2, No.1, Feb. 2016, Pub. Date: Feb. 24, 2016
Genetic Variability Among Soybean [Glycine max (Merrill)] Varieties in Nitrogen Fixation in Five Ghanaian Soils
Pages: 15-21 Views: 1152 Downloads: 625
[01] Phanuel Y. Klogo, Agro Enterprise Development Department, Ho Polytechnic, Ho, Ghana.
[02] Patrick K. Avumegah, Agro Enterprise Development Department, Ho Polytechnic, Ho, Ghana.
[03] Seth K. A Danso, Soil Science Department, University of Ghana, Legon, Accra.
[04] Mawutor Glover, Agro Enterprise Development Department, Ho Polytechnic, Ho, Ghana.
[05] Victor Owusu-Gyimah, Agricultural Engineering Department, Ho Polytechnic, Ho, Ghana.
The high diversity of bradyrhizobia in tropical soils offers great advantage in making the nodulation of an array of tropical legumes possible. The nodulation, N2 fixed (using 15N) and growth of three uninoculated promiscuous soybean varieties, TGx 813-6D, TGx 1448-2E and TGx 1903-8F and Bragg, and a non-promiscuous American-type soybean variety were examined in five soil typesin a greenhouse at the University of Ghana, Legon. Except for the Bekwai soil that contained 4.0 x 101 Bradyrhizobium (sp.) cells g-1 soil, the other soils contained 103 or more cells g-I soil (1.0,4.0, 4.6 and 6.0 x 103 cells g-1 soil in Aveime, Hatso, Adenta and Chichiwere soils, respectively). Both plant genotype and soil type significantly influenced nodulation, withTGx813-6D and TGx 1903-8F generally forming more nodules than TGx 1448-2E, whose nodulation was similar (P<0.05) to that of Bragg. However, step-wise regression indicated that the native Bradyrhizobium population was most important in determining the outcome of nodulation, accounting for 56.90% of the total variation in nodule numbers. The contribution of Bradyrhizobium population to N2 fixation was even higher, accounting for 74.46% the variation in % N fixed. Highest nodulation and N2 fixation occurred in Chichiwere and Adenta, followed by Hatso and Aveime soils in that order with the TGx varieties on average deriving > 60% N from fixation, compared to 30-46%, for Bragg in these soils. No nodulation and N2 fixation occurred in soybean in the Bekwai soil with lowest bradyrhizobia population, and averaged for all five soils, N2 fixation contributed less than 50% of the N in the TGx varieties. The ability of promiscuous soybean varieties to nodulate with indigenous bradyrhizobia was highly variable and was strongly influenced by the abundance of bradyrhizobia cells in soil. With average of less than 50% N fixed by indigenous strains, there is scope for enhancing the symbiotic performance of TGx varieties in many soils through inoculation with more effective Bradyrhizobium strains.
Bradyrhizobium, 15N Isotope, Nitrogen Fixation, Nodulation, Promiscuous, Soybean Variety
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