Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.1, No.2, Apr. 2015, Pub. Date: Apr. 2, 2015
Efficacy of Hargel (Solanostemma argel (Del) hayne) Shoots Extract Against the Broad Bean Beetle (Bruchidius incarnatus)
Pages: 52-61 Views: 4265 Downloads: 2066
Authors
[01] Mohammed Osman Mohammed Sir El Khatim, Department of Crop Protection Faculty of Agriculture, Omdurman Islamic University, Omdurman, Sudan.
[02] Azhari Omer Abdelbagi, Department of Crop Protection Faculty of Agriculture, University of Khartoum, Khartoum, Sudan.
Abstract
Laboratory studies were conducted to evaluate the efficacy of aqueous and organic extracts of the Hargel shoots against the adult stage of the broad bean beetle Bruchidius incarnatus in the Sudan. Hargel shoots were extracted sequentially by organic solvents of increasing polarity (Petroleum ether, Ethyl acetate and Ethanol) as well as directly by with distilled water or ethanol. Extracts were tested at concentrations ranging between 1% to 10%. The evaluated efficacy parameters included; mortality, repellency, antifeedant and effects on weight loss in stored faba bean seeds (Vicia faba L.). The tests were conducted in Petri dishes (9 cm i.d) and plastic cups (capacity 200 ml) and the obtained data were subject to the analysis of variance (ANOVA) and further by probit analysis.The results of the mortality data indicated that the sequential extraction with ethanol was the most potent against the test insect as shown by its low LD50 of 0.33%. Various types of Hargel shoot extracts induced significant dose dependent repellency against the broad bean beetle. The highest 24 hours repellency was caused by the aqueous extract as indicated by its low ED50 value of 10.6 %.The different Hargel shoot extracts also induced significant antifeedant action against the test insect. The lowest Feeding ratio (Fr) of 0.017 was recorded in aqueous extract treated faba bean seeds while the lowest percentage weight loss (1.7%) was noticed on stored faba bean treated with petroleum ether extract.
Keywords
Tribolium castaneum, Hargel Shoots, Mortality, Repellency and Antifeedant Effects
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