Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.4, No.1, Mar. 2018, Pub. Date: Aug. 31, 2018
Studies on Protease Producing Bacterial Biodiversity from Soil Collected from Arba Minch University, Abaya Campus, Southern Ethiopia
Pages: 1-6 Views: 358 Downloads: 273
Authors
[01] Temam Abrar Hamza, Biotechnology Department, College of Natural Sciences Arba Minch University, Arba Minch, Ethiopia.
[02] Nurdin Nassir Azmach, Department of Statistics, College of Natural Sciences, Arba Minch University, Arba Minch, Ethiopia.
Abstract
Microbes are small microscopic organisms and they are found in every habitat such as soil, water, food, and animal intestines, as well as in more extreme environment under extreme conditions such as rocks, glaciers, hot springs, and deep-sea vents. Hydrolytic enzymes are one of the most important groups of microbial products that have various application in various industries. The aims of the present study was study the biodiversity of protease producing bacteria from soil collected from the study area (Arba Minch University, Abaya campus). Skim milk agar medium was used for isolation and screening of protease producer. Totally 157 bacterial strain were isolated from the study area, 38 of them are protease producer. The diversity of protease producer were studied based on colony morphology, Gram staining, endospore staining and biochemical tests. These organisms were then identified in accordance with the Bergey’s Manual of Determinative Bacteriology. Accordingly, 15 Bacillus sp., 9 Staphylococcus sp., 5 Psuedomonas sp., 4 Micrococcus sp., 2 Corynebacterium sp., 2 Lactobacillus and 1 Streptococcus sp. were protease producer identified in the present study. Among four potential protease producing isolates, three strains are belongs to Bacillus spp, however, AMUa31 isolate was found to be Psuedomonas sp. Isolate AMUa38 and AMUa12 produce relatively higher clear zone with 26.5 and 16mm size respectively. The use of microorganisms to produce industrially important enzymes has a number of technical and economic advantages and in recent years has become the predominant mode of enzyme production. In this regard, members of the genus Bacillus produce a large variety of extracellular proteases which have significant application in various industries.
Keywords
Biodiversity, Protease, Bacteria, Isolation, Abaya Campus
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