Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.5, No.1, Mar. 2019, Pub. Date: Apr. 10, 2019
Antagonistic Activity of Pseudomonas Species Isolated from the Rhizosphere of Cowpea Against Some Fungal Pathogens
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[01] Fatuyi Olanipekun Ekundayo, Department of Microbiology, Federal University of Technology, Akure, Nigeria.
[02] Sefunmi Alaofin, Department of Microbiology, Federal University of Technology, Akure, Nigeria.
Soil samples collected from Crop, Soil and Pest departmental farm were subjected to chemical and microbiological analysis. The soil samples were then placed in polyethylene bags, inoculated with 0.2g of mancozeb, Benomyl and both Mancozeb and Benomyl respectively. Cowpea seeds (Vigna unguiculata) were planted in each of the experimental setup. Bacteria were isolated from the rhizosphere of cowpea treated with Mancozeb, Benomyl and combination of both of them. Pseudomonas species isolated from the rhizosphere of cowpea treated with Benomyl or / and Mancozeb was used to inhibit some fungal pathogen of plant origin. The chemical analysis revealed the soil to be acidic and high in Calcium, Phosphorus and Magnesium. The bacterial count of the soil before the planting of cowpea was 4.0 X 108, 10.33±3.09a in Mancozeb treated soil and 6.03±0.888b in Mancozeb and Benomyl treated soil. The bacterial isolated from the soil before planting of cowpea were Actinomyces viscosus, Pasteurella species, Corynebacteria renale, Pseudomonas capacia, Bacillus pumilus, Pseudomonas stutzeri, Bacillus subtilis while Bacillus pumilus, Corynebacterium renale, Bacillus macerans, Actinomyces viscosus, Pseudomonas stutzeri, Bacillus subtilis, Corynebacterium ovis, Bacillus licheniformis, Bacillus firmus, Pasteurella multocida, Bacillus coagulans, Pseudomonas pseudomallei were isolated from the rhizosphere of soil treated with Benomyl only. The bacterial isolated from the rhizosphere of soil treated with mancozeb only were Clostridium botulinum, Staphylococci aureus, Plesiomonas shigelloides, Chromobacterium volaceum, Actinomyces viscosus, Pseudomonas stutzeri, Corynebacterium ovis, Bacillus licheniformis, Bacillus pantothenticus, and Bacillus firmus while Bacillus licheniformis, Pasteurella multicida, Bacillus subtilis, Pseudomonas florescens, Pseudomonas capacia and Pasteurella mutocida from the rhizosphere of soil treated with both mancozeb and benomyl. Pseudomonas pseudomallei isolated from the rhizosphere of soil treated with Benomyl gives 28.5-25.0% inhibition at 24 to 96 hours against Rhizotonia solani while it inhibited Sclerotium rolfsii at 60 – 60.8% between 24 to 48 hours. Pseudomonas strutzeri gives 33.0-5.0% inhibition against Rhizotonia solani at 24 to 96 hours.
Antifungal, Benomyl, Mancozeb, Pathogens
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