Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.5, No.1, Mar. 2019, Pub. Date: Apr. 10, 2019
Antagonistic Activity of Pseudomonas Species Isolated from the Rhizosphere of Cowpea Against Some Fungal Pathogens
Pages: 1-6 Views: 1219 Downloads: 324
Authors
[01] Fatuyi Olanipekun Ekundayo, Department of Microbiology, Federal University of Technology, Akure, Nigeria.
[02] Sefunmi Alaofin, Department of Microbiology, Federal University of Technology, Akure, Nigeria.
Abstract
Soil samples collected from Crop, Soil and Pest departmental farm were subjected to chemical and microbiological analysis. The soil samples were then placed in polyethylene bags, inoculated with 0.2g of mancozeb, Benomyl and both Mancozeb and Benomyl respectively. Cowpea seeds (Vigna unguiculata) were planted in each of the experimental setup. Bacteria were isolated from the rhizosphere of cowpea treated with Mancozeb, Benomyl and combination of both of them. Pseudomonas species isolated from the rhizosphere of cowpea treated with Benomyl or / and Mancozeb was used to inhibit some fungal pathogen of plant origin. The chemical analysis revealed the soil to be acidic and high in Calcium, Phosphorus and Magnesium. The bacterial count of the soil before the planting of cowpea was 4.0 X 108, 10.33±3.09a in Mancozeb treated soil and 6.03±0.888b in Mancozeb and Benomyl treated soil. The bacterial isolated from the soil before planting of cowpea were Actinomyces viscosus, Pasteurella species, Corynebacteria renale, Pseudomonas capacia, Bacillus pumilus, Pseudomonas stutzeri, Bacillus subtilis while Bacillus pumilus, Corynebacterium renale, Bacillus macerans, Actinomyces viscosus, Pseudomonas stutzeri, Bacillus subtilis, Corynebacterium ovis, Bacillus licheniformis, Bacillus firmus, Pasteurella multocida, Bacillus coagulans, Pseudomonas pseudomallei were isolated from the rhizosphere of soil treated with Benomyl only. The bacterial isolated from the rhizosphere of soil treated with mancozeb only were Clostridium botulinum, Staphylococci aureus, Plesiomonas shigelloides, Chromobacterium volaceum, Actinomyces viscosus, Pseudomonas stutzeri, Corynebacterium ovis, Bacillus licheniformis, Bacillus pantothenticus, and Bacillus firmus while Bacillus licheniformis, Pasteurella multicida, Bacillus subtilis, Pseudomonas florescens, Pseudomonas capacia and Pasteurella mutocida from the rhizosphere of soil treated with both mancozeb and benomyl. Pseudomonas pseudomallei isolated from the rhizosphere of soil treated with Benomyl gives 28.5-25.0% inhibition at 24 to 96 hours against Rhizotonia solani while it inhibited Sclerotium rolfsii at 60 – 60.8% between 24 to 48 hours. Pseudomonas strutzeri gives 33.0-5.0% inhibition against Rhizotonia solani at 24 to 96 hours.
Keywords
Antifungal, Benomyl, Mancozeb, Pathogens
References
[01] M., Aboagye, Egbadzor, K. F., Ofori, K., Yaboah, L. M., Opoku-Agymen, M. O., Danquah, E. Y., Offei, S. K. (2014). Diversity in 113 cowpea (Vigna unguiculata (L) walp) accession assessed with 458 SNP markers. Springer Plus. 3: 541. ISSN 2193-1801.
[02] Owusu, E. Y., Akromah, R., Denwar, N. N., Danquah, J. A., Kusi, F. and Haruna, M. (2018). Inheritance of Early Maturity in Some Cowpea (Vigna unguiculata (L) walp) Genotypes under Rain Fed Condition in Northern Ghana. Advances in agriculture. Volume 2018, Article ID8930259.
[03] National Research Council (2006). Cowpea. Lost crops of Africa: Vegetables. Washington DC. The National Academies Press. 2: 104-117. ISBN 978-0-309-10333-6.
[04] Abbas, A. (2015). Management of plant diseases. Research Gate. Page 1.
[05] Onifade, A. K. (2010). Phytotherapy, A random walk through a random forest. An inaugural lecture presented at the Federal University of Technology, Akure. On Feb. 16, 2010. Pgs 30.
[06] Savary, S., Ficke, A., Aubertot, J. N. and Hollier, C. (2012). Crop losses due to diseases and their implication for global food production and food security. Springer. Pp 5. ISSN 1876-4517.
[07] Association of Official and Analytical Chemists, (2000). Official method of Analysis. 13th edition. Washington DC.
[08] Olutiola, P. O., Famurewa, O. and Senntag, H. G. (2000). An introduction to General microbiology, Hygiene institute Der UniversitalHeideberg. Federal Republic of Germany. Pp 267.
[09] Willey, J. M., Sherwood, L. M. and Woolverton, C. J. (2008). Prescott, Harley, and Klein’s Microbiology, NY, McGraw Hill, 7th Edition. 7: 123-156.
[10] Das, P. M., Devi, V. P., and Yasmine, Y. (2015). A study on the Antagonistic potential of Bacteria against Phytopathogenic fungi. International Journal of Pharmaceutical Sciences Review and Research. 34 (1): 191-193.
[11] Wang, R., Zhang, H., Suu, L., Gaofu, Q., Chen, S. and Zhao, X. (2017). Microbial Community composition is related to Soil Biological and Chemical properties and bacterial wilt Outbreak. Scientific Report. Nature Publishing Group. Doi: 10.1038/S41598-017-00472-6.
[12] Susilowati, A. and Wiyono, S. (2011). Potential of Pseudomonas isolated from Soybean Rhizosphere as Biocontrol against Soil Borne Phytopathogenic fungi. HAYATI Journal of Biosciences. 18 (20): 51-56.
[13] Savati, T. R., and Tiwani, P. (2015). Invitro Antagonistic Activity of Pseudomonas species against Rhizoctonia solani. African Journal of Microbiology Research. 9 (25): 1622-1628.
[14] Paramegeetham, C. and Babu, G. P. (2012). Antagonistic Activity of Fluorescent Pseudomonads against a Polyphagous Soil Born Plant Pathogen- Sclerotium rolfsii. Scientific Report. 1: 436. Doi: 4172/scientific report. 436.
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