Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.5, No.1, Mar. 2019, Pub. Date: Apr. 29, 2019
Germination and Mitochondrial Respiration Rate in Vigna unguiculata (Bean) Seeds Exposed to Crude Oil
Pages: 29-34 Views: 44 Downloads: 19
[01] Stella Oghomwen Olubodun, Department of Medical Biochemistry, School of Basic Medical Sciences, University of Benin, Benin City, Nigeria.
[02] George Edaghogho Eriyamremu, Department of Biochemistry, Faculty of Life Sciences, University of Benin, Benin City, Nigeria.
Vigna unguiculata (bean) seeds were treated with 0%, 2%, 5% 10% crude oil fractions [whole crude (WC), water soluble fraction (WSF) and water insoluble fraction (WIF)] for 21 days. Crude oil stress in some plant species leads to the formation of reactive oxygen species (ROS). However, evidence are lacking and very limited studies explore the measurement of mitochondrial respiration in developing plants during environmental stress. This study investigated the growth and mitochondrial respiration rate in bean seeds exposed to crude oil. Experiments carried out in equal amounts of developing roots after 7, 14, and 21 days post germination (DPG) were performed to determine plant height, radicle/root length and mitochondrial respiration rate. After 21 DPG, seedling height of bean in 10% WC, WSF, and WIF decreased significantly. Radicle/root lengths of bean seedlings in 10% WC, WSF and WIF at 7 DPG, also decreased. Decreased respiration rate was observed in the roots of bean in different crude oil fractions when compared with control but this was not found to be significant (P<0.05) with increase in crude oil contamination or increase in DPG. In conclusion, the germinating seedlings revealed reduced growth and development with display of morphological and biochemical variations in the radicle/roots in crude oil stress. We suggest that reduction of root length and mitochondrial respiration rate of the bean seedlings may be responsible for the observed decrease in growth and development in crude oil stress. There is a need for public enlightenment on the deleterious effects of crude oil spills in the environment and law for proper clean-up.
Crude Oil Stress, Germination, Mitochondrial Respiration Rate, Vigna unguiculata (Bean)
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