Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.5, No.2, Jun. 2019, Pub. Date: Jun. 25, 2019
Antifungal Activities of Ocimum Gratissimum Against Water-based Paint Spoilage Molds
Pages: 76-83 Views: 26 Downloads: 14
Authors
[01] Nwafor Helen Chisom, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria.
[02] Orji Michael Uchenna, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria.
[03] Okafor Ugochukwu Chukwuma, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria.
[04] Anyaegbunam Bede Chike, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria.
Abstract
Water-based paint spoilage is caused by actions of some microorganism, resulting in the deterioration of paint quality and coating integrity. This research work focused on water-based paint spoilage molds, and the use of Ocimum gratissimum in the control of spoilage molds of water-based paint. Twenty paint samples were examined microbially and physicochemically. pH, Specific gravity and viscosity were the physicochemical parameters assessed in the research. In addition the phytoconstituents of O. gratissimum were analysed, and the extract was incoroporated into paints for further antifungal studies. The fungal isolates from the paint samples were identified further using wet mount method (lactophenol cotton blue staining), slide culture test, and ITS inter-specific region sequencing using 17SrDNA sequencing. Four different paint samples were divided into groups A, B, C and D. These were monitored for a period of four months for physicochemical changes, microbial changes, colour shift, odour and their fungal counts were monitored by plating on Sabouraud’s Dextrose Agar. The molecular identification revealed the fungi to be Cladosporium tenuissimum, Rhizopus sp., Aspergillus niger and Aspergillus tamari, with C. tenuissimum giving the highest occurrence of 55%. Antifungal activity of O. gratissimum methanolic extract against C. tenuissimum gave a minimum inhibitory concentration of 50mg/ml and minimum fungicidal concentration (MFC) of 200mg/ml with nystatin (22.4) as standard. The phytoconstituents of the plant extract were flavonoids, tannins, alkaloid, saponin, steroid and terpenoids. Incorporation of the extract in water-based paint contaminated with C. tenuissimum, revealed that the extract was able to control the growth of the fungi. This research work showed that O. gratissimum has the potential to be used as a natural biocide in paint production.
Keywords
Paints, Ocimum gratissimum, Antifungal Activity, Biocides, Spoilage Molds
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