Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.6, No.1, Mar. 2020, Pub. Date: Jan. 14, 2020
RT-qPCR Expression of Fatty Acid Binding Protein1 and 2 (FABP1&2) Genes in Fatty Liver Tissue Overfed Goose
Pages: 22-26 Views: 60 Downloads: 15
Authors
[01] Rashid Habiballa Osman, Faculty of Animal Production, West Kordofan University, El-Nuhud, Sudan; Faculty of Animal Science and Technology, Yangzhou University, Yangzhou, China.
[02] Mojahid Abdallah Abdalhag, Faculty of Animal Science and Technology, Yangzhou University, Yangzhou, China; Faculty of Agricultural Technology & Fish Science, Al Neelain University, Khartoum, Sudan.
[03] Tamador Algam, Faculty of Agricultural Technology & Fish Science, Al Neelain University, Khartoum, Sudan.
[04] Ahmed Kamel, Faculty of Veterinary Science, Seuz Canal University, Seuz, Egypt.
[05] Wafaa Babiker Zomrawi, Faculty of Agriculture and Natural Resources, University of Bakht Elruda, Al-Dueim, Sudan.
[06] Mohammed Ibrahim Ahmed, Faculty of Veterinary Science, West Kordofan University, El-Nuhud, Sudan.
[07] Dao Qing Gong, Faculty of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Abstract
Fatty acid-binding proteins 1, 2, 3, 4 and L-FABP occur in the liver in a high concentration where it is involved in the fatty liver functions. In this study, diet content cooked maize was used to overfeed Landes geese for 19 days. The expression levels of FABP1/2 were determined by using quantitative PCR in goose liver and primary hepatocytes. The expression level of FABP2 gradually decreased with overfeeding time in the livers of the geese, while FABP1 was extremely down-regulated after one week of overfed. The expression level of FABPs1 mRNA was the lowest in the overfeeding group at 89 days. In primary hepatocytes, the data indicated that the expression of FABP1/2 were inhibited by high levels of glucose (25-50 mM). In addition, insulin (50-100mM) inhibited FABP1 in goose hepatocytes, while it was not significantly (p 0.05) for FABP2. These findings suggest that the expression of the FABP1 gene is hyper-effect to hyperglycemia and hyperinsulinemia that causing inhibition of the gene in goose fatty liver. For fatty acid treatment, the expression levels of FABP1/2 were not significantly altered in fatty acid (0.25 or 0.5mM) treatment. All data were normalized by the GAPDH gene and analyzed by the 2-ΔΔCt method.
Keywords
Fatty Liver, Goose, FABP 1/2 genes, Overfeeding, Primary Hepatocytes
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