[01] Olabode Isaiah Ogunsina, Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Ondo State, Nigeria.

The speedy increase of antimicrobial resistance has become a worldwide problem. This has compelled the need to search for novel antimicrobial molecules. Lannea acida extracts are rich sources of potential antimicrobial components base on the ethnopharmacological survey. This study investigates the antimicrobial properties of methanolic extract of Lannea acida, flavonoid-rich and alkaloid-rich extracts of Lannea acida. Agar well diffusion and time-kill kinetic against the bacterial assays were used to determine the antimicrobial activity of the extracts against selected test organisms. The effect of methanolic extract and flavonoid-rich extract on the growth of B. subtilis, S. aureus, E. coli, K. pneumoniae, E. aerogenes were investigated and experienced slow growth in the presence of methanolic crude extract and flavonoid-rich extracts in comparison to organism growing in medium alone. Preliminary secondary metabolite screening revealed the presence of tannins, flavonoids, triterpenoids, quinones, and alkaloids in the methanolic extracts of Lannea acida, for the first one hour, the growth rates for the microorganisms remained the same regardless of whether it was growing in the presence or absence of extracts. After the one hour, the growth rate then rose exponentially for bacterial organisms growing in media alone but remained suppressed for organism growing in the presence of extracts for the next 7 hours with percent OD values. The extracts demonstrated activities against the Grams-positive and Grams-negative bacterial, which showed a bacteriostatic effect in killing rate kinetics. Lannea acida extracts exhibited antimicrobial activity and may contain bioactive compounds which may serve as potential antibacterial and antifungal agents.

Lannea acida, Antibacterial, Microorganism

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