Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.1, No.4, Aug. 2015, Pub. Date: Jun. 2, 2015
Use of Fruits and Raffia Palm Sap (Raffia hookeri) in Chilled Bull Semen Extenders in Cameroon
Pages: 142-149 Views: 2700 Downloads: 1395
Authors
[01] P. H. Bayemi, Institute of Agricultural Research for Development, Yaounde, Cameroon.
[02] N. R. Banla, University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Production, Dschang, Cameroon.
[03] I. Leinyuy, Institute of Agricultural Research for Development, Yaounde, Cameroon.
[04] A. T. Niba, University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Production, Dschang, Cameroon.
[05] V. M. Nsongka, Institute of Agricultural Research for Development, Yaounde, Cameroon.
[06] G. Mario, Animal Production and Health Section, International Atomic Energy Agency (IAEA), Vienna, Austria.
[07] M. Shamshuddin, Animal Production and Health Section, International Atomic Energy Agency (IAEA), Vienna, Austria.
Abstract
A study was carried out to investigate the effect of substituting egg yolk in egg-yolk-citrate (EYC) extenders with extracts from Tomato and Pawpaw fruits as well as Coconut milk and Raffia palm sap on the motility of bull spermatozoa. Semen collected from Holstein Friesian bulls by the artificial vagina was extended with the proportions of egg yolk in egg-yolk-citrate extenders substituted at 25% (75% egg yolk: 25% Substituent), 50% (50% egg yolk:50% Substituent), 75% (25% egg yolk:75% Substituent) and 100% (no egg yolk in extender) and stored under refrigeration conditions (4-8oC). Results show that coconut milk (43.4%) and pawpaw juice (41.25%) led to the highest number of progressively motile sperms (P< 0.05) without evidence of difference between them (P > 0.05). They were followed by tomato Juice (40.35%), fresh raffia palm sap (40.40) and Trehalose (34.68%). Substitution of egg yolk at 75% had highest individual motility while total substitution (100%) had the lowest (P<0.0001). Meanwhile 50% and 75% substitution led to similar sperm progressive motility (P>0.05). Sperms retained 70% motility up to 15 days with coconut milk, 14 days with pawpaw juice, 14 days with fresh raffia sap, 13 days with tomato juice and 10 days with Trehalose. In the egg yolk treatment control, the drop below 70% was after the 4th day. Room temperature semen could be used only within 24H in all treatments. Fruits extenders performed better than egg yolk extenders, thus showing the beneficial effects of fruits in improving sperm motility. This is the first time such a result is reported. More studies need to be carried out on avoiding the sharp fall in motility 2 weeks after extension, on chemical concentrations of used fruits and in fertility of sperms with these extenders. In Cameroon where eggs are relatively more expensive, extenders may adequately be made with tropical fruits.
Keywords
Bull Semen, Egg Yolk, Fruits Extenders, Sperm Motility
References
[01] Bayemi, P.H., I. Leinyuy V.M. Nsongka, E.C. Webb & A.L. Ebangi, 2010. Viability of cattle sperm under different storage conditions in Cameroon. Journal of Tropical Animal Health Production. 42:1779–1783
[02] Bayemi, P.H., Bryant, M.J., Perera, B.M.A.O., Mbanya, N.J., Cavestany, D., Webb, E.C., 2005a. Milk production in Cameroon: a review. Livestock research for rural development 17, 6.www.cipav.org.co/lrrd/lrrd17/6/cont1706.
[03] Bayemi, P.H., Bryant,M.J., Pingpoh, D., Imele, H., Mbanya, J., Tanya,V., Cavestany, D., Awoh, J., Ngoucheme, A., Sali, D., Ekoue, F., Njakoi, H.,Webb, E.C., 2005b. Participatory rural appraisal of dairy farms in the northwest province of Cameroon. Livestock research for rural development 17, 6. www.cipav.org.co/lrrd/lrrd17/6/cont1706.
[04] Bouchard G.F., Morris J.K., Sikes J.D., Youngquist R.S., 1990. Effects of storage temperature, cooling rates and two different semen extenders on canine spermatozoal motility. Theriogenology; 34:147–57.
[05] Brown R.M., 1992. An update of artificial insemination with fresh, chilled, and frozen semen. Probl Vet Med; 4:445–52.
[06] Crumley H., 2009. What Foods Increase Glutathione Levels? The Journal of Nutrition, May 2004, 134, 1114-1119. http://www.livestrong.com/article/32331-foods-increase-glutathione-levels/
[07] Ebangi, A.L.,Tawah, C.L., Mbah, D.A., Tanya, V.N.,Ndi, C., Ekue,F.N., Messine, O., Ottou, J.B.B., Enoh., M.B., 2004. Mortalities amongst different genotypes of crossbred dairy calves in a tropical environment. Journal of the Cameroon Academy of Science vol. 1, No. 2: 117–124.
[08] Foote R.H., Brockett C.C., Kaproth M.T., 2002. Motility and fertility of bull sperm in whole milk extender containing antioxidants. Animal Reproduction Science 71: 13–23.
[09] Goodman M.F., Cain J.L., 1993. Retrospective evaluation of artificial insemination with chilled extended semen in the dog. Journal of Reproduction Fertility; 47(Suppl.):554.
[10] Hu, J.-H., Zan L.S., Zhao X.L., Li Q.W., Jiang Z.L., Li Y.K., and Li X. 2010. Effects of trehalose supplementation on semen quality and oxidative stress variables in frozen-thawed bovine semen. Journal of Animal Science, 88:1657-1662.
[11] IAEA, 2007. Improving the Reproductive Management of Dairy Cattle Subjected to Artificial Insemination. Vienna, Austria: IAEA. 148pp.
[12] Ibrahim H. 1998. Small Ruminant Production Techniques. ILRI Manual 3. ILRI (International Livestock Research Institute), Nairobi, Kenya. 207 pp.
[13] Iguer-Ouada M., Verstegen J.P., 2001. Long-term preservation of chilled canine semen: effect of commercial and laboratory prepared extenders. Theriogenology, 14: 671-684.
[14] McGinnis L.K., Zhu L., Lawitts J.A., Bhowmick S., Toner M., Biggers J.D., 2005. Mouse sperm desiccated and stored in trehalose 3 medium without freezing. Biological Reproduction. 73(4):627-33
[15] Megwa S. 1984. Aspects of the preparation of formulated palm wine. M.Sc. Dissertation. University of Nigeria, Nsukka.
[16] Minepia, Ministre de l'Elevage, des Pêches et des Industries Animales, 2005. Evolution et Situation Actuelle de l’Elevage au Cameroun. Rapport de synthèse. 41pp
[17] Minresi, Ministère de la recherché Scientifique et de l’Innovation, 2007. Journées d’excellence de la recherche Scientifique et de l’Innovation au Cameroun (JERSIC). Prix spécial du président de la république, (2007) 3-5 juillet.
[18] Munsi, M.N., Bhuiyan M.M.U., Majumder S., and Alam M.G.S., 2007. Effects of Exogenous Glutathione on the Quality of Chilled Bull Semen. Reprod Dom Anim 42: 358–362.
[19] Ndambi, A. and Bayemi, P.H., 2008. Cameroon-dairy sector and chain profile. In. Hemme et al. IFCN Dairy Report 2008. International Farm Comparison Network, IFCN Dairy Research Center, Kiel, Germany
[20] Ngwa, Tarla Divine, 2010. Effect of egg yolk concentrations, cooling methods and ph on chilled bovine semen characteristics. Dissertation, FASA, University of Dschang, Cameroon.
[21] Okafor N., 1978. Microbiology and biochemistry of oil palm wine. NAIFOR Newsletter 12: 8- 6.
[22] Pingpoh, D.P., Mbanya, J., Ntam, F., Malaa, D.K., 2007. Some risk management practiced among the beef cattle farmers of the North West Province of Cameroon: Effect on technology dissemination. 8pp.
[23] SAS/STAT, 2002, Statistical Analyses System (8th ed., Volume 2). Cary NC: SAS Institute Inc.
[24] Shamsuddin M., Ahmed J.U., Alam MGS, Modak P.C., 1987: Effect of age of semen on conception rate in cattle under farm condition. Bang Veterinary Journal 21: 51–58.
[25] Sinha M.P., Sinha A.K., Prasad R.L., 1996: The effect of glutathione on the motility, enzyme leakage and fertility of frozen goat semen. Animal Reproduction Science 41: 237–243.
[26] Verstegen, J.P., Onclin K., Iguer-Ouada, M., 2005. Long-term motility and fertility conservation of chilled canine semen using egg yolk added Tris–glucose extender: in vitro and in vivo studies. Theriogenology 64: 720–733.
[27] Yadolah Dodge, 2008. The Concise Encyclopedia of Statistics. University of Neuchâtel, Switzerland. Springer reference, 612pp.
600 ATLANTIC AVE, BOSTON,
MA 02210, USA
+001-6179630233
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - American Institute of Science except certain content provided by third parties.