Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.1, No.4, Aug. 2015, Pub. Date: May 28, 2015
Fusidic Acid and Rifampicin Resistant Strains of Methicillin Resistant Staphylococcus aureus (MRSA) from Northwestern Nigerian Hospitals
Pages: 132-136 Views: 2030 Downloads: 1031
[01] Abdulhadi Sale Kumurya, Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, Kano, Nigeria.
Background: Glycopeptides have been the last in the line of antibiotics used to treat serious MRSA infections. The emergence and spread of multi-resistant methicillin resistant Staphylococcus aureus (MRSA) strains, especially those resistant to fusidic acid and rifampicin in Nigerian hospitals is of concern. Objectives: The main objectives of the study were to determine the resistance rates of fusidic acid and rifampicin among MRSA strains in Northwestern Nigerian hospitals. Methods: In this study PCR was performed for detection of mecA gene from S. aureus isolates. Antibiotic susceptibility testing using disc susceptibility testing and minimum inhibitory concentration (MIC) were performed on 423MRSA isolates from 8 health institutions across Northwestern Nigeria. The isolates were obtained from clinical samples collected for duration of two years from February 2008 to April 2010. The resistance was confirmed by determination of MIC of fusidic acid and rifampicin. Staphylococcus aureus ATCC 25923 was used as a reference control organism. Results: 0f 432 MRSA isolates, 21(4.9%) and 27(6.4%) were found to be resistant to fusidic acid and rifampicin respectively. All the 21 and 27 isolates were confirmed to be resistant with MIC of >25mumg/L and >45mumg/L to fusidic acid and rifampicin respectively. Conclusion: The information gained from the epidemiology of fusidic acid and rifampicin- resistant isolates can help the hospitals infection control teams understand the epidemiology of these organisms in their institutions. Surveillance of these strains should be carried out to prevent further spread of the clone. Increase resistance to fusidic acid and rifampicin will further reduce the already limited treatment options for MRSA infections.
Fusidic Acid, Rifampicin, MRSA, Resistance, Northwestern Nigeria
[01] Adebayo, S., Johnson, L. and Deboye, K. (2006). Antimicrobial Susceptibility Patterns of Staphylococcus aureus and Characterization of MRSA in Southwestern Nigeria. Wounds 18(4):77-84.
[02] Andrews JM (2001). Determination of minimum inhibitory concentrations. J. Antimicrob. Chemother. 48(suppl 1): 5-16.
[03] Anna-Kaarina, J., SannaLaakso, P., Anne sAittakorpi, M., Lindfors, L., Huopaniemi, H. and Minna, M. (2009). Rapid identification of bacterial pathogens using aPCR- and microarray-based assay. BMC Microbiology 9:161.
[04] Bignardi, G. E., Woodford, N., Chapman, A., Johnson, A. P. and Speller, D. C. E. (1996). Detection of the mec-A gene and phenotypic detection of resistance In Staphylococcus aureus isolates with border ine or low-level methicillin Resistance. Journal of Antimicrobial Chemotherapy 37: 53—63.
[05] Cavassini, M. A., Wenger, K., Jaton, D. S., Blane, and J. Bille. (1999). Evaluation of MRSA-Screen, a simple anti-PBP-2a slide latex agglutination kit, for rapid detection of methicillin resistance in Staphylococcus aureus. J. Clin. Microbiol. 37: 1591-1594.
[06] Chang SC, Hsieh SM, Chen ML, Sheng WH, Chen YC. Oral fusidic acid fails to eradicate methicillin-resistant Staphylococcus aureus colonization and results in emergence of fusidic acid-resistant strains. DiagnMicrobiol Infect Dis 2000. 36:131–6.
[07] Cheebrough, M. (2000). District Laboratory Practice in Tropical Countries 2. pp 180-242.
[08] Cambridge Uni. Press.Ghebremedhin, B., W. Konig, W. Witte, K.J. Kardy, P.M. Hawkey, and B. Konig, (2007).
[09] Subtyping of ST22MRSA-IV (Barnim epidemic MRSA strain) at a university clinic in Germany from 2002 to 2005. J. Med. Microbiol. 54:365-375.
[10] Howden BP, Grayson ML (2006). Dumb and dumber - The potential waste of a useful antistaphylococcal agent: Emerging fusidic acid resistance in Staphylococcus aureus. Clin. Infect. Dis. 42(3): 394-400.
[11] Isenberg, H. D. (1998). Agar screening test to detect oxacillin (methicillin)-resistantStaphylococcus Spp. In: Essential procedures for clinical microbiology.ASM Press WashintonDCpp 232-234.
[12] Kesah, C., Ben Redjeb, S., Odugbemi, T.O., Boye, C.S.B., Dosso, M.Y., Achola, J.O. et al. (2003). Prevalence of methicillin-resistant Staphylococcus aureus. In eight African hospitals and Malta. Clin Microbiol infect. 9: 153-156.
[13] Kucers A (1997). Fusidate sodium. In: Kucers A, Crowe SM, Grayson ML, HoyJF. The use of antibiotics. 5th ed. Oxford: Butterworth-Heinemann. 580–6.
[14] NCCLS (2006). National Committee for Clinical Laboratory Standards, 2006. Performance standards for antimicrobial susceptibility tests. Smith informational supplement, NCCLSdocumentM100-56. Wayne, PA.Norazah A., Lim V. K. E., Koh Y. T., Rohani M. Y., Zuridah H., Spender P.P et al. (2002).
[15] Molecular fingerprinting of fusidic acid- and ripamficin-resistant strains of methicillin-resistant Staphylococcus aureus (MRSA) from Malaysian hospitals. Journal of Medical Microbiology 51:1113-1116.
[16] O’Brien, F. G., Botterill, C. I., Endersby, T. G., Lim, R.L.G., Gruubb, W. B., Gustafson, J. E.(1998). Heterogenous expression of fusidic acid resistance in Staphylococcus aureus with plasmid or chromosomally encoded fusidic acid resistance genes. Pathology 30:299-303.
[17] Perez, R. E., Claverie, M. F., Villar, J. and Méndez, Á. (2001).Multiplex PCR for Simultaneous Identification of Staphylococcus aureus and Detection of Methicillin and Mupirocin Resistance. Journal of Clinical Microbiology 39(11): 4037-4041.
[18] Ravenscroft JC, Layton A, Barnham M. Observations on high levels of fusidic acid resistant Staphylococcus aureus in Harrogate, North Yorkshire, UK. ClinExpDermatol 2000; 25:327–30.
[19] Sakharkar MK, Jayaraman P, SoeWM, Chow VTK, Sing LC, Sakharkar KR (2009). In vitro combinations of antibiotics and phytochemicals against Pseudomonas aeruginosa. J. Microbiol. Immunol., 42(5): 364-370.
[20] Sakoulas G, Moellering RC (2008). Increasing Antibiotic Resistance among MethicillinResistant Staphylococcus aureus Strains. Clin. Infect. Dis. 46(Supplement 5): S360-S367.
[21] Sievert DM, Rudrik JT, Patel JB, McDonald LC, Wilkins MJ, Hageman JC (2008).Vancomycin-resistant Staphylococcus aureus in the United States, 2002-2006. Clin. Infect. Dis., 46(5): 668-674.
[22] Schmitz, F. J., Fluit, A. C., Hatner, D (2000). Development of resistance to ciprofloxacin, rifampicin and mupirocin in methicillin-susceptible and resistant Staphylococcus aureus isolates. Antimicrob Agents chemother 44:3229-3231.
[23] Shopsin, B. and Kreiswirth, B.N. (2003). Molecular epidemiology of Methicillin-resistant Staphylococcus aureus. Emerging Infections Disease 7:323-326.
[24] Tiemersma, E. W., Brozwaer, S.L., Lyytikainen, O., Degener, J.E., Schrijnemakers. P., BruinsmaN. et al. (2004). Methicillin-resistant Staphylococcus aureus in Europe. 1999-2002. EmergInfect Dis. 10: 1627-1634.
[25] Tiwari H, Sen M (2006). Emergence of vancomycin resistant Staphylococcus aureus (VRSA) from a tertiary care hospital from northern part of India. BMC Infect. Dis., 6(1): 156.
[26] Toma, E and Barriault, D. (1995). Antimicrobial activity of fusidic acid and disk diffusion susceptibility testing criteria for gram-positive cocci. J. Clin. Microbiol. 33:1712-1715.
[27] Yu HH, Kim KJ, Cha JD, Kim HK, Lee YE, Choi NY, You YO (2005). Antimicrobial activity of berberine alone and in combination with ampicillin or oxacillin against methicillin-resistant Staphylococcus aureus. J. Med. Food. 8(4): 454-461.
[28] Zhao X, Eisner W, Perl-Rosenthal N, Kreiswirth B, Drlica K (2003). Mutant Prevention Concentration of Garenoxacin (BMS-284756) for Ciprofloxacin-Susceptible or -Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 47(3): 1023-1027.
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