American Journal of Clinical Neurology and Neurosurgery
Articles Information
American Journal of Clinical Neurology and Neurosurgery, Vol.1, No.2, Sep. 2015, Pub. Date: Aug. 6, 2015
Nitric Oxide and Interleukin-6 Production in Patients with Transient Cerebral Microcirculatory Disturbances
Pages: 86-91 Views: 2123 Downloads: 569
Authors
[01] Maria N. Starodubtseva, Department of Medical and Biological Physics, Gomel State Medical University, Gomel, Belarus.
[02] Natallia V. Halinouskaya, Department of Neurology and Neurosurgery, Gomel State Medical University, Gomel, Belarus.
[03] Nadezhda M. Halubykh, Research Laboratory, Gomel State Medical University, Gomel, Belarus.
[04] Evgenii V. Voropaev, Research Laboratory, Gomel State Medical University, Gomel, Belarus.
[05] Vasiliy B. Smychek, National Science and Practice Centre of Medical Assessment and Rehabilitation, Gorodische, Belarus.
Abstract
Reactive nitrogen species are well known to be important participants of cerebral stroke development. Low concentration of NO is produced by constitutive NO-synthase under physiological conditions, whereas high concentration of NO is produced by inducible NO-synthase of the stimulus-activated cells of the immune system and endothelium. Based on the concept of two cell states (resting and activated) with different NO production rates we have analyzed NO metabolite (NOx) concentration in plasma of patients with transient cerebral microcirculatory disturbances (TCMD) simultaneously with the levels of inflammatory markers (superoxide dismutase activity and concentration of interleukins (IL-6 and IL-8) and C-reactive protein) and studied the effect of a standard 10-day therapeutic course on them. The sample of patients with TCMD included 24 patients with transient ischemic attack (TIA) and 19 patients with acute hypertensive encephalopathy (AHE). The NOx concentration was measured by the modified Griess method. The presence of two groups with low and high NOx concentration in plasma was detected for the studied sample both in the beginning and in the end of patient’s hospital stay (high NOx plasma samples were found in 18% of cases on Day 1 and 27% of cases on Day 10). The majority of subjects with high plasma NOx concentration on Day 1 were AHE patients (63%) and on Day 10 were TIA patients (78%), correspondingly, The high NOx plasma samples were associated with low superoxide dismutase activity (p=0.045) and increased IL-6 level (p=0.003). Our study’s results suggest that the analysis of NOx concentration changes during a therapeutic course is useful for improving the diagnosis and future prognosis of patients with TCMD.
Keywords
Stroke, Cerebral Hypertension, Transient Ischemic Attack, Peroxynitrite, Nitrite/Nitrate Ions, Interleukin-6, C-Reactive Protein
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