Bioscience and Bioengineering
Articles Information
Bioscience and Bioengineering, Vol.1, No.4, Oct. 2015, Pub. Date: Dec. 14, 2015
Accumulation of Cu(II) and Pb(II) in Three Rhodophytes of the Genus Gracilaria and the Impact of the Metals on the Algal Physiology
Pages: 106-111 Views: 899 Downloads: 347
[01] Luqman Abu Bakar, School of Fundamental Science, Universiti Malaysia Terengganu, Kuala Terengganu, Terengganu, Malaysia.
[02] Hazlina Ahamad Zakeri, School of Fundamental Science, Universiti Malaysia Terengganu, Kuala Terengganu, Terengganu, Malaysia.
The accumulation of two heavy metals, copper (Cu(II)) and lead (Pb(II)) in the tissues of three Gracilaria species (Rhodophyta): G. edulis, G. manilaensis and G. salicornia and the impacts of the metals on the algal maximal quantum yield (i.e. Fv/Fm), relative growth and chlorophyll (chl) a content were studied. The algae were exposed to 1 mg L-1 of the metals individually for 8 hrs. Results showed that the three species of Gracilaria reacted differently against the two metals. For every kilogram of thallus, > 1000 mg of Cu(II) and Pb(II) was collected for G. edulis and G. manilaensis. Furthermore, both algae has a Bioconcentration Factor (BCF) value of >1 for both metals. G. salicornia, however, collected < 1000 mg Cu(II) and Pb(II) for every kilogram of thallus, and has a BCF value of >1 for Cu(II) and < 1 for Pb(II). These values indicate that all algae are good accumulators of Cu(II) while G. edulis and G. manilaensis are good accumulators of Pb(II) but G. salicornia, on the other hand, is an excluder of Pb(II). There was a reduction of the algal Fv/Fm in both metals, with the highest reduction observed for G. manilaensis in Cu(II). Relative growth of the algae was also reduced in both metals. Cu(II) induced the synthesis of chl a in G. edulis and G. salicornia but inhibited chl a synthesis in G. manilaensis while Pb(II) induced the production of chl a in all algae.
Gracilariaceae, Heavy Metals, Quantum Yield, Relative Growth, Bioconcentration Factor
[01] Iqbal, M. and Saeed, A. (2007). Production of an immobilized hybrid biosorbent for the sorption of Ni (II) from aqueous solution. Process Biochemistry, 42: 148-157.
[02] Aydin, H., Bulut, Y. and Yerlikaya, C. (2008). Removal of copper (III) from aqueous solution by adsorption onto low-cost adsorbents. Journal of Environmental Management, 87: 37-45.
[03] Connan, S. and Stengel, D. B. (2011). Impacts of ambient salinity and copper on brown algae: 1. Interactive impacts on photosynthesis, growth, and copper accumulation. Aquatic Toxicology, 104, 97-107.
[04] Qufei, L. and Fashui, H. (2009). Impacts of Pb 2+ on the structure and function of photosystem II of Spirodela polyrrhiza. Biological Trace Element Research, 129: 251-260.
[05] Zayadan, B. K., Sadvakasova, A. K., Hassan, M. M. and Beisenova, A. Z. (2013). Bioremediation of heavy metal contaminated water by microalgae. International Journal of Biology and Chemistry, 5: 32-35.
[06] Sánchez-Rodríguez, I., Huerta-Diaz, M. A., Choumiline, E., Holguin-Quinones, O. and Zertuche-Gonzalez, J. A. (2001). Elemental concentrations in different species of seaweeds from Loreto Bay, Baja California Sur, Mexico: implications for the geochemical control of metals in algal tissues. Environmental Pollution, 114:145–160.
[07] Rajamani, S., Siripornadulsil, S., Falcao, V., Torres, M., Colepicolo, P. and Sayre, R. (2007). Phycoremediation of heavy metals using transgenic microalgae. Advances in Experimental Medicine and Biology, 616: 99-109.
[08] Sekabira, K., Oryem Origa, H., Basamba, T. A., Mutumba, G. and Kakudidi, E. (2010). Application of algae in biomonitoring and phytoextraction of heavy metals contamination in urban stream water. International Journal of Environmental Science and Technology, 8: 115-128.
[09] Inskeep, W. P. and Bloom, P. R. (1985). Extinction coefficients of chlorophyll a and b in N,N-dimethylformamide and 80% acetone. Plant Physiology, 77: 483-485.
[10] Baumann, H. A., Morrison, L. and Stengel, D. B. (2009). Metal accumulation and toxicity measured by PAM-Chlorophyll fluorescence in seven species of marine macroalgae. Ecotoxicology and Environmental Safety, 72: 1063–1075.
[11] dos Santos, R. W., Schmidt, E. C., de L Felix, M. R., Polo, L. K., Kreusch, M., Pereira, D. T., Costa, G. B., Simioni, C., Chow, F., Ramlov, F., Maraschin, M., and Bouzon, Z. L. (2014) Bioabsorption of cadmium, copper and lead by the red macroalga Gelidium floridanum: Physiological responses and ultrastructure features. Ecotoxicology and Environmental Safety, 105: 80–89.
[12] Yruela, I. (2005) Copper in plants. Brazilian Journal of Plant Physiology, 17: 145-146.
[13] Machado, M. D., Lopes, A. R. and Soares. E. V. (2015). Responses of the alga Pseudokirchneriella subcapitata to long-term exposure to metal stress. Journal of Hazardous Materials, 296: 82-92.
[14] Sharma, P. and Dubey, R. S. (2005) Lead toxicity in plants. Brazilian Journal of Plant Physiology, 17: 35-52.
[15] Pawlik-Skowronska, B. (2002). Correlations between toxic Pb impacts and production of Pb-induced thiol peptides in the microalga Stichococcus bacillaris. Environmental Pollution, 119: 119–227.
[16] Baker, A. J. M. and Brooks, R. R. (1989). Terrestrial higher plants which hyper accumulate metallic elements - Review of their distribution, ecology and phytochemistry. Biorecovery, 1: 81-126.
[17] Zayed, A., Gowthaman, S. and Terry, N. (1998). Phytoaccumulation of trace elements by wetland plants: I. Duckweed. Journal of Environmental Quality, 27: 715-721.
[18] Nazir, A., Malik, R. N., Ajaib, M., Khan, N. and Siddiqui, M. F. (2011). Hyperaccumulators of heavy metals of industrial areas of Islamabad and Rawalpindi. Pakistan Journal of Botany, 43: 1925-1933.
[19] Maxwell, K. and Johnson, G. N. (2000). Chlorophyll fluorescence – a practical guide. Journal of Experimental Botany, 51: 659-668.
[20] Baker, N. R. (2008). Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annual Review of Plant Biology, 59: 89-113.
[21] Lichtenthaler, H. K., Buschmann, C. and Knapp, M. (2005). How to correctly determine the different chlorophyll fluorescence parameters and the chlorophyll fluorescence decrease ratio Rfd of leaves with the PAM fluorometer. Photosynthetica, 43: 379-393.
[22] Wei, Y., Zhu, N., Lavoie, M., Wang, J., Qian, H. and Fu, Z. (2014). Copper toxicity to Phaeodactylum tricornutum: a survey of the sensitivity of various toxicity endpoints at the physiological, biochemical, molecular and structural levels. Biometals, 27: 527-537.
[23] Cambrollé, J., Mateos-Naranjo, E., Redondo-Gómez, S., Luque, T. and Figueroa, M. E. (2011). Growth, reproductive and photosynthetic responses to copper in the yellow-horned poppy, Glaucium flavum Crantz. Environmental and Experimental Botany, 71: 57–64.
[24] Bernardini, A., Salvatori, E., Guerrini, V., Fusaro, L., Canepan, S. and Manes, F. (2016) Impacts of high Zn and Pb concentrations on Phragmites australis (Cav.) Trin. Ex. Steudel: photosynthetic performance and metal accumulation capacity under controlled conditions. International Journal of Phytoremediation, 18: 16-24.
[25] Hazlina, A. Z. and Luqman, A. B. (2013). Copper-, lead- and mercury-induced changes in maximum quantum yield, chlorophyll a content and relative growth of three Malaysian green macroalgae. Malaysian Journal of Fundamental and Applied Sciences, 9: 16-21.
[26] Szivák, I., Behra, R. and Sigg, L. (2009). Metal-induced reactive oxygen species production in Chlamydomonas reinhardtii (Chlorophyceae). Journal of Phycology, 45: 427-435.
[27] Asada, K. (2006). Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiology, 141: 391-396.
[28] Hazlina, A. Z. and Nor Shuhanija, S. (2013). Physiological and biochemical responses of a Malaysian red alga, Gracilaria manilaensis treated with copper, lead and mercury. Journal of Environmental Research and Development, 7: 1246-1253.
[29] Jiang, W. S., Liu, D. H. and Hou, W. Q. (2001). Hyperaccumulation of cadmium by roots, bulbs and shoots of garlic (Allium sativum L.). Bioresource Technology, 76: 9-13.
[30] Brown, M. T. and Newman, J. E. (2003). Physiological responses of Gracilariopsis longissima (S. G. Gmelin) Steentoft, L. M. Irvine and Farnham (Rhodophyceae) to sub-lethal copper concentrations. Aquatic Toxicology, 64: 201–213.
[31] Malar, S., Vikram, S. S., Favas, P. J. C., and Perumal, V. (2014). Lead heavy metal toxicity induced changes on growth and antioxidative enzymes level in water hyacinths [Eichhornia crassipes (Mart.)]. Botanical Studies, 55: 54.
[32] Scheidegger, C., Behra, R. and Sigg, L. (2011). Phytochelation formation kinetics and toxic impacts in the freshwater alga Chlamydomonas reinhardtii upon short- and long-term exposure to lead(III). Aquatic Toxicology, 101: 423-429.
[33] John, R., Ahmad, P., Gadgil, K. and Sharm, S. (2009). Heavy metal toxicity: Effect on plant growth, biochemical parameters and metal accumulation by Brassica juncea L. International Journal of Plant Production, 3: 65-76.
[34] Janssen, C. and Heijrick, D. (2003). Algal toxicity testing for environmental risk assessment of metals: physiological and ecological considerations. Environmental Contamination and Toxicology, 178: 23-52.
[35] Bossuyt, B. T. A. and Janssen, C. R. (2004). Long-term acclimation of Pseudokirchneriella subcapitata (Korshikov) Hindak to different copper concentrations: changes in tolerance and physiology. Aquatic Toxicology, 68: 61-74.
[36] Zhang, X., Ervin, E. H. and Schmidt, R. E. (2005). The role of leaf pigment and antioxidant levels in UV-B resistance of dark- and light-green Kentucky bluegrass cultivars. Journal of the American Society for Horticultural Science, 130: 836–841.
[37] Cenkci, S., Cigerci, I. H., YildIz, M., Özay, C., Bozdag, A. and Terzi, H. (2010). Lead contamination reduces chlorophyll biosynthesis and genomic template stability in Brassica rapa L. Environmental and Experimental Botany, 67: 467–473.
[38] Pourraut, B., Shahid, M., Dumat, C., Winterton, P. and Pinelli, E. (2011). Lead uptake, toxicity and detoxification in plants. Reviews of Environmental Contamination and Toxicology, 213: 113–136.
[39] Shakya, K., Chettri, M. K. and Sawidis, T. (2008). Impact of heavy metals (copper, zinc and lead) on the chlorophyll content of some mosses. Environmental Contamination and Toxicology, 54: 412-421.
[40] Han, T., Kang, S. H., Park, J. S. and Lee, H. K. (2008). Physiological responses of Ulva pertusa and U. armoricana to copper exposure. Aquatic Toxicology, 86: 176-194.
MA 02210, USA
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - 2017 American Institute of Science except certain content provided by third parties.