Bioscience and Bioengineering
Articles Information
Bioscience and Bioengineering, Vol.4, No.4, Dec. 2018, Pub. Date: Dec. 6, 2018
Anti-Mycobacterial Activity on Middlebrook 7H10 Agar of Selected Congolese Medicinal Plants
Pages: 68-77 Views: 1628 Downloads: 395
Authors
[01] Gédéon Ngiala Bongo, Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo; Department of Veterinary Microbiology, Parasitology and Immunology, College of Veterinary and Biomedical Sciences, Sokoine University of Agriculture, Morogoro City, Tanzania.
[02] Huruma Nelwike Tuntufye, Department of Veterinary Microbiology, Parasitology and Immunology, College of Veterinary and Biomedical Sciences, Sokoine University of Agriculture, Morogoro City, Tanzania.
[03] Joseph Malakalinga, Department of Veterinary Microbiology, Parasitology and Immunology, College of Veterinary and Biomedical Sciences, Sokoine University of Agriculture, Morogoro City, Tanzania.
[04] Koto-te-Nyiwa Ngbolua, Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[05] Aaron Lelo Pambu, Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[06] Tshiama Claudine, Teaching and Administration in Nursing Care, Higher Institute of Medical Techniques, Kinshasa City, Democratic Republic of the Congo.
[07] Clément Mutunda Mbadiko, Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[08] Gisèle Kafuti Makengo, Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[09] Pius Tshimankinda Mpiana, Department of Chemistry, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[10] Théophile Fundu Mbemba, Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[11] Rudovick Kazwala, Department of Veterinary Medicine and Public Health, College of Veterinary and Medical Sciences, Sokoine University of Agriculture, Morogoro City, Tanzania.
Abstract
In recent years, Tuberculosis (TB) has re-emerged as a serious public health problem worldwide. The disease spreads more easily in overcrowded settings and in conditions of malnutrition and poverty. The emergence of multidrug resistant and lengthy therapy reduces the patient compliance which comprises TB control strategies. In the current study, the leaves of Terminalia ivorensis, Carapa procera, Fagara macrophylla, Anacardium occidentale, Ficus spp. and Drepanoalpha® were extracted using petroleum ether, ethyl acetate and methanol in order to assess their anti-mycobacterial activity against Mycobacterium tuberculosis H37Rv and Mycobacterium tuberculosis spp. on Middlebrook 7H10 agar using a qualitative approach where the activity was determined by the presence or the absence of growth on the plate. The phytochemical screening was used for the identification of the major groups of compounds found in the extracts. The methanolic extract displayed a good activity on both strains than the petroleum ether and ethyl acetate. The presence of alkaloids, flavonoids, tannins, saponins, anthocyanins, quinones known to be of medicinal importance points out a possible source for anti-mycobacterial agents to address the problem of multidrug resistance. The in vitro findings of this study provide a partial support for the use of these plants in the management of various infectious diseases as lead to drug discovery and should be reiterated and recommended for a clinical trial using an animal model.
Keywords
Tuberculosis, Middlebrook 7H10 Agar, Anti-mycobacterial Activity, Phytochemical Screening, Medicinal Plants
References
[01] Lwilla, F., Schellenberg, D., Masanja, H., Acosta, C., Galindo, C., Aponte, J., Egwaga, S., Njako, B., Ascaso, C., Tanner, M. and Alonso, P. Evaluation of efficacy of community-based vs. institutional-based direct observed short-course treatment for the control of tuberculosis in Kilombero district, Tanzania. Tropical Medicine and International Health, 2003, 8 (3): 204–210.
[02] Vikrant, A. A Review on Anti-Tubercular Plants. International Journal of PharmTech Research, 2011, 3 (2): 872-880.
[03] Ngbolua, K. N., Gedeon, N. B., Masengo, C. A., Djolu, R. D., Pius, T. M., Virima, M., Lassa, K. and Huruma N. T. Ethnobotanical survey and Ecological study of Plants resources used in Folk medicine to treat symptoms of Tuberculosis in Kinshasa City, Democratic Republic of the Congo. Journal of Modern Drug Discovery and Drug Delivery Research, 2014, 1 (4): 1-6.
[04] Muna M. B. and Fauzia R. E. G. Antibacterial Activity of Medicinal Aqueous Plant Extracts against Mycobacterium tuberculosis. Malaysian Journal of Microbiology, 2012, 8 (3): 203-206.
[05] Gizachew, E. Y., Giday, M. and Teklehaymanot, T. Antimycobacterial Activities of Selected Ethiopian Traditional Medicinal plants used for treatment of symptoms of Tuberculosis. Global Advanced Research Journal of Medicinal Plants, 2013, 2 (2): 022-029.
[06] World Health Organization report (WHO). Global Tuberculosis. WHO Geneva, Switzerland, 2014, 171pp.
[07] Kaswa, K. M., Bisuta, S., Kabuya, G., Lunguya, O., Ndongosieme, A., Muyembe, J. J., Van Deun, A. and Boelaert, M. (2014). Multi Drug Resistant Tuberculosis in Mosango, a Rural Area in the Democratic Republic of Congo. Plos One, 9 (4): 1-4.
[08] Bunalema, L. Anti-mycobacterial activity and acute toxicity of Erythrina abyssinica, Cryptolepis sanguinolenta and Solanum incanum. Unpublished Dissertation for Award of MSc Degree at the University of Makerere, Kampala, Uganda, 2010, 53pp.
[09] Molly, A., James, J., Misra, S. C., Sagadevan, L. D. M., Veettil, A. K. T. and Thankamani, V. Anti-mycobacterial activity of the plant extracts of Alstonia scholaris. International Journal of Current Pharmaceutical Research, 2011, 4 (1): 40-42.
[10] Cooke, G. J. Public Health Africa: A Report of the Center for Strategic & International Studies (CSIS) Global Health Policy Center. CSIS, 2009, 16pp.
[11] Gandhi R. N., Nunn P., Dheda K., Schaaf H. S., Zignol M., Dick van Soolingen D. V., Jensen P. and Bayona J. Multidrug-resistant and extensively drug-resistant tuberculosis: a threat to global control of tuberculosis. The Lancet, 2010, 375: 1830-1843.
[12] Tijjani, M. B.; Bello, I. A.; Aliyu, A. B.; Olurishe, T., Maidawa, S. M.; Habila, J. D. and Balogun, E. O. Phytochemical and antibacterial studies of root extract of Cochlospermum tinctorium A. rich. (Cochlospermaceae). Research Journal of Medicinal Plant, 2009, 3: 16-22.
[13] Ngbolua K. N., Inkoto C. L., Bongo G. N., Lengbiye E. M., Lufuluabo G. L., Masengo C. A., Tshibangu D. S. T., Tshilanda D. D. and Mpiana P. T. Phytochemistry and Bioactivity of Annona reticulata L. (Annonaceae): A Mini-review. South Asian Research Journal of Natural Products, 2018, 1 (1): 1-11
[14] Edeoga H. O., Okwu D. E. and Mbaebie B. O. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology, 2005, 4 (7): 685-688.
[15] Mativandlela, S. P. N. Antituberculosis activity of flavonoids from Galenia africana L. var. Africana. Unpublished Dissertation for award of PhD degree, at the Faculty of Natural and Agricultural Science, University of Pretoria, South Africa, 2009, 189pp.
[16] Mpiana, P. T., Ngbolua, K. N., Tshibangu, D. S. T., Mazasa, P. P., Gbolo, B. Z., Atibu, E. K., Kadima, J. N. and Kasali, F. M. (2014). In vitro Antisickling and Radical Scavenging Activities of a Poly-herbal Formula (Drepanoalpha®) in Sickle Cell Erythrocyte and Acute Toxicity Study in Wistar Albino Rats. European Journal of Medicinal Plants, 2014, 4 (10): 1251-1267.
[17] Bongo G., Tuntufye H., Ngbolua K. N., Malakalinga J., Claudine T., Pambu A., Mwanza F., Mbadiko C., Makengo G., Tshilanda D., Mpiana P., Mbemba T. and Kazwala R. Comparative Antimycobacterial Activity on Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia. Journal of Diseases and Medicinal Plants, 2017, 3 (5): 88-96. doi: 10.11648/j.jdmp.20170305.12
[18] Al-Daihan, S., Al-Faham M., Al-shawi N., Rawan Almayman R., Brnawi, A., Zargar, S. and Shafi Bhat R. (2013). Antibacterial activity and phytochemical screening of some medicinal plants commonly used in Saudi Arabia against selected pathogenic microorganisms. Journal of King Saud University - Science, 25 (2): 115-120.
[19] Bukatuka F. C., Ngombe K. N., Mutwale K. P., Moni B. M., Makengo K. G., Pambu L. A., Bongo N. G., Mbombo M. P., Musuyu M. D., Maloueki U., Koto-te-Nyiwa Ngbolua and Mbemba F. T. Bioactivity and Nutritional Values of Some Dioscorea Species Traditionally Used as Medicinal Foods in Bandundu, DR Congo. European Journal of Medicinal Plants, 2016, 14 (1): 1-11.
[20] Bongo G. N., Ngbolua K. N., Ashande C. M., Karume K. L., Mukiza J., Tshilanda D. D., Tshibangu D. S. T., Ngombe N. K., Mbemba T. F. & Mpiana P. T. Pharmacological screening of Gymnanthemum coloratum (Willd.) H. Rob. & B. Kahn (Compositae) and Terminalia ivorensis A. Chev. (Combretaceae) from DR Congo: Spotlight on the antisickling, antibacterial and anti-diabetic activities. Tropical Plant Research, 2017, 4 (3): 441–448.
[21] Madikizela, B., Aderogba, M. A., Finnie, J. F., and Staden, V. J. Isolation and characterization of antimicrobial compounds from Terminalia phanerophlebia Engl. and Diels leaf extracts. Journal of Ethnopharmacology, 2014, 156: 228-34.
[22] Cobbinah, A. E. Antioxidant and Antibacterial activities of the chemical constituents of Terminalia ivorensis CHEV. Unpublished dissertation for Award of MSc Degree at the Department of Chemistry, Faculty of Physical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, 2008, 139pp.
[23] Coulibaly, K., Zirihi, G. N., Guessennd-kouadio, N., Oussou, K. R. and Dosso, M. Antibacterial properties studies of trunk barks of Terminalia ivorensis (Combretaceae), a commercial and medicinal species, on some methicillin-resistant Staphylococci spp strains. African Health Sciences, 2014, 14 (3): 753-756.
[24] Agbedahunsi, M. J., Anao, I., Adewunmi, O. C., and Croft, L. S. Trypanocidal properties of Terminalia ivorensis A. Chev. (Combretaceae). African Journal Traditional, Complementary and Alternative Medicines, 2006, 3 (2): 51-56.
[25] Kuete, V., Tabopda, T. K., Ngameni, B., Nana, F., Tshikalange, T. E. and Ngadjui, T. B. A, timycobacterial, antibacterial and antifungal activities of Terminalia superba (Combretaceae). South African Journal of Botany, 2010, 76 (1): 1-21.
[26] Eldeen, I. M. S. and Staden, V. J. Antimycobacterial activity of some trees used in South African traditional medicine. South African Journal of Botany, 2007, 73 (2): 248-251.
[27] Asres, K., Bucar, F., Edelsbrunner, S. Kartnig, T., Hoger, G., and Thiel, W. Investigations on antimicrobial activity of some Ethiopian medicinal plants. Phytotherapy Research, 2001, 15: 323-326
[28] Mann, A., Kolo I., Oyewale, O. A,, Joseph O. Amupitan, O. J., Majekodumi, O. F. and Okogun, I. J. Antimycobacterial Friedelane-terpenoid from the Root Bark of Terminalia avicenioides. American Journal of Chemistry, 2011, 1 (2): 52-55.
[29] Bongo N. G. Assessment of the anti-mycobacterial activity of selected Congolese Medicinal Plants. Unpublished dissertation for the award of MSc degree, Department of Veterinary Microbiology, Parasitology and Immunology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro, Tanzania, 2016, 101 pp.
[30] Missah, B. Larvicidal and anti-plasmodial constituents of Carapa procera DC. (Meliaceae) and Hyptis suaveolens L. POIT (Lamiaceae). Unpublished dissertation for the award of MSc degree, Department of Pharmacognosy Faculty of Pharmacy and Pharmaceutical Sciences, Kwaume Nkrumah University of Science and Technology: College of Health Sciences, Kumasi, Ghana, 2014, 100 pp.
[31] Bishola, T. T. Etude phytochimique et détermination de quelques activités biologiques de Pentaclethra macrophylla Benth (Fabaceae), Carapa procera Decne (Meliaceae) et Garcinia punctata Decne (Clusiaceae) (Phytochemical screening and assessment of biological activities of Pentaclethra macrophylla Benth (Fabaceae), Carapa procera Decne (Meliaceae) and Garcinia punctata Decne (Clusiaceae)). Unpublished Dissertation for Award of BSc Degree at the University of Kinshasa, Kinshasa, DRC, 2013, 125pp.
[32] Pereira, T. B., Rocha e Silva, F. L., Amorim, C. N. R., Melo, R. S. M., Zacardi de Souza, C. R., Eberlin, N. M., Lima, S. E., Vasconcellos, C. M. and Pohlit, M. A. In vitro and in vivo anti-malarial activity of limonoids isolated from the residual seed biomass from Carapa guianensis (andiroba) oil production. Malaria Journal, 2014, 13: 137.
[33] Olugbuyiro, A. O. J., Moody, O. J. and Hamann, M. T. Anti-infective potential of some medicinal plants used in South-West Nigeria. International Journal of Innovations in Bio-Sciences, 2013, 3 (3): 104-110.
[34] Kayoka K. N. P., Eloff N. J. Chikwelu L. Obi C. L. and Lyndy J. M. (2016). Antimycobacterial Activity and Low Cytotoxicity of Leaf Extracts of Some African Anacardiaceae Tree Species. Phytotherapy research, 2016, 1-11.
[35] Luo X., Piresb D., Ainsac A. J., Graciac B., Mulhovoe S., Duartea A., Anesb E., Maria-Jose U. Ferreira (2011). Antimycobacterial evaluation and preliminary phytochemical investigation of selected medicinal plants traditionally used in Mozambique. Journal of Ethnopharmacology, 137: 114-120.
[36] Onasanwo, S. A., Fabiyi, T. D., Oluwole, F. S. and Olaleye, S. B. Analgesic and anti-inflammatory properties of the leaf extracts of Anacardium occidentale in the laboratory rodents. Nigerian Journal of Physiological Sciences, 2016, 27 (1): 65-71.
[37] Dare, S. S., Hamman, W. O., Musa, S., Goji, A. D. T., Oyewale, A. A., Abba, S. and Ezekiel, I. Effects of aqueous extracts of Anacardium occidentale (Cashew) leaf on pregnancy outcome of Wistar rats. International Journal of Animal and Veterinary Advances, 2010, 3 (2): 77-82.
[38] Suneetha R. and Lakshman K. (2017). Cashew apple (Anacardium occidentale L.) therapeutic benefits, processing and product development: An overview. The Pharma Innovation Journal, 6 (7): 260-264.
[39] Gaya, C. H., Kawaka, J. F., Muchungi, A. and Ngeranwa, J. J. Variation of alkaloids in the Kenyan Zanthoxylum gilletii (De Wild Waterman). African Journal of Plant Science, 2013, 7 (9): 438-444.
[40] Lansky, E. P., Paavilainen, M. H., Pawlus, D. A. and Newmann, A. R. Ficus spp. (fig): Ethnobotany and potential as anticancer and anti-inflammatory agents. Journal of Ethnopharmacology, 2008, 119: 195-213.
[41] Mariita, M. R., Callistus K. P. O. O., Oguge, O. N. and Paul O Okemo, O. P. Antitubercular and Phytochemical Investigation of Methanol Extracts of Medicinal Plants Used by the Samburu Community in Kenya. Tropical Journal of Pharmaceutical Research, 2010, 9 (4): 379-385.
[42] Ajayi, I. A., Ajibade O. and Oderinde R. A. Preliminary Phytochemical Analysis of some Plant Seeds. Research Journal of Chemical Sciences, 2011, 1 (3): 1-5.
[43] Spatafora, C. and Tringali, C. Bioactive metabolites from the bark of Fagara macrophylla. Phytochemical analysis, 2011, 8: 139-142.
[44] Yadav, R. N. S. and Agarwala, M. Phytochemical analysis of some medicinal plants. Journal of Phytology, 2011, 3 (12): 10-14.
[45] Aguilar, M. Y., Yero, M. O., Arias, E. A., Soto, F. R. and Navarro, V. M. Composición química y tamizaje fitoquímico del polvo de hojas y retoños del Anacardium occidentale L. (marañón) (Chemical composition and phytochemical screening of leaf and shoot powder of Anacardium occidentale L. (cashew tree)). Revista Cubana de Plantas Medicinales, 2012, 17 (1): 1-10.
[46] Ojezele, O. M. and Agunbiade, S. Phytochemical Constituents and Medicinal Properties of Different Extracts of Anacardium Occidentale and Psidium Guajava. Asian Journal of Biomedical and Pharmaceutical Sciences, 2013, 3 (16): 20-23.
[47] Nwankwo, I. U. and Ukaegbu-Obi, K. M. Preliminary phytochemical screening and antibacterial activity of two Nigerian medicinal plants (Ficus asperifolia and Terminalis catappa). Journal of Medicinal Plant and Herbal Therapy, 2014, 2: 1-5.
[48] Salem, M. Z. M., Salem, A. Z. M., Camacho, L. M. and Ali, M. H.. Antimicrobial activities and phytochemical composition of extracts of Ficus species: An overview. African Journal of Microbiology Research, 2013, 7 (33): 4207-4219.
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