Chemistry Journal
Articles Information
Chemistry Journal, Vol.1, No.5, Oct. 2015, Pub. Date: Nov. 23, 2015
Broad-Spectrum Antimicrobial Activity of Silver Nanoparticles in Different Types of Chitosan Matrices
Pages: 165-171 Views: 2762 Downloads: 1257
Authors
[01] M. Carmen Rodríguez-Argüelles, Inorganic Chemistry Department, Faculty of Chemistry, University of Vigo, Vigo, Spain.
[02] Noelia González-Ballesteros, Inorganic Chemistry Department, Faculty of Chemistry, University of Vigo, Vigo, Spain.
[03] Gregorio Rodríguez-Domínguez, Inorganic Chemistry Department, Faculty of Chemistry, University of Vigo, Vigo, Spain.
[04] Marco Campanini, Institute of Materials for Electronics and Magnetism, National Research Council (IMEM-CNR), Parma, Italy.
[05] Lucia Nasi, Institute of Materials for Electronics and Magnetism, National Research Council (IMEM-CNR), Parma, Italy.
[06] Iria Vázquez, Functional Biology and Health Sciences Department, Microbiology Area, Faculty of Biology, University of Vigo, Vigo, Spain.
[07] Carmen Sieiro, Functional Biology and Health Sciences Department, Microbiology Area, Faculty of Biology, University of Vigo, Vigo, Spain.
Abstract
Due to the problem of resistance of many infectious agents to the usual treatments, this study addresses the ways of obtaining and using new chitosan-silver nanomaterials as antimicrobial agents. Chitosans of medium (CSM) and high (CSH) molecular weights were used as matrices in the formation of small silver nanoparticles (AgNP) when mixed with AgNO3 under different conditions, such as using the polymer (CS) in acetic acid solutions, as nanoparticles (CS-NP) or in films. The films were prepared by evaporation of the CS-AgNO3 mixtures at different temperatures and in all cases AgNP was formed. When acetic acid solutions of CS were boiled in the presence of AgNO3 CS-NP were formed containing AgNP (Ag@CS-NP). It is evident that the size and form of presentation of CS are not factors that can significantly affect the formation of small AgNP. AgNP inserted in different CS matrices presented significant antimicrobial activity (MIC between 0.6-4 μg/mL) against four Gram-positive and five Gram-negative bacteria, and also against three yeasts. It is interesting to emphasize the highest activities achieved for Ag@CSH-NP against B. cereus (0.6 μg/mL) and Ag@CSM-NP against P. pastoris (0.7 μg/mL). Although the antimicrobial activity was dependent on the strain assayed, the overall tendency observed was that the nanocomposites made with CSH are more effective than those prepared with CSM.
Keywords
Antimicrobial,Chitosan,Nanoparticle,Silver,TEM, UV-Vis
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