International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.1, No.4, Aug. 2015, Pub. Date: Jul. 9, 2015
Effect of Vernonia amygdalina on Some Biochemical Indices in Dimethylnitrosamine (DMN)-Induced Liver Injury in Rats
Pages: 99-105 Views: 4409 Downloads: 1597
[01] Usunobun Usunomena, Department of Basic Sciences (Biochemistry Unit), Faculty of Basic and Applied Sciences, Benson Idahosa University, Benin City, Edo State, Nigeria.
[02] Okolie P. Ngozi, Department of Biochemistry, Faculty of Life Sciences, University of Benin, Benin City, Nigeria.
[03] Eze G. Ikechi, Department of Anatomy, School of Basic Medical Sciences, College of Medicine, University of Benin, Benin City, Nigeria.
This experiment pertains to the protective role of Vernonia amygdalina pre-treatment on some biochemical indices in dimethylnitrosamine (DMN)-induced liver damage in male albino rats. Four (4) groups of six (6) rats each were used for the study. Group 1served as control, group 2 and 3 were pre-treated with 400mg/kg Vernonia amygdalina for one week while group 3 and 4 each received single dose of 20mg/kg DMN (orally) after one week. The rats were sacrificed 48hrs after DMN administration. In rats administered 20mg/kg DMN, liver damage was clearly shown by increased activities of serum hepatic marker enzymes namely aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), alkaline phosphatase (ALP), and gamma glutamyltransferase (GGT), increased lipid profile parameters such as total cholesterol and triglycerides as well as increased level of lipid peroxidation indices, malondialdehyde (MDA) in liver. The toxic effect of DMN was also indicated by significantly decreased levels of antioxidants such as superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH). However, in rats pre-treated with 400mg/kg Vernonia amygdalina and dosed thereafter with DMN, there were significant reversal in the activities of serum hepatic marker enzymes, lipid profiles, lipid peroxidation and significant restoration of antioxidant levels in the liver when compared to DMN-alone treated rats. Histopathological studies in the liver of rats also showed that Vernonia amygdalina pre-treatment markedly reduced the toxicity of DMN and significantly preserved the normal histological architecture of the tissue. The findings of this study suggest that pre-treatment with Vernonia amygdalina leaves has a protective and beneficial effect on liver subjected to DMN-induced oxidative stress, possibly by decreasing lipid peroxidation and enhancing endogenous antioxidant production.
Antioxidant, Dimethylnitrosamine, Necrosis, Oxidative Stress, Vernonia Amygdalina
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