International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.1, No.6, Dec. 2015, Pub. Date: Dec. 14, 2015
Isolation and Identification of Equine Influenza H3N8 Virus from Clinical Cases in Khartoum State, Sudan
Pages: 298-301 Views: 2498 Downloads: 1113
Authors
[01] Wegdan H. A., Veterinary Research Institute, Khartoum, Sudan.
[02] Intisar K. Saeed., Veterinary Research Institute, Khartoum, Sudan.
[03] Shaza M. M., Veterinary Research Institute, Khartoum, Sudan.
[04] Algezoli O. A., Veterinary Research Institute, Khartoum, Sudan.
[05] Ballal A., Veterinary Research Institute, Khartoum, Sudan.
[06] Ihsan H. A., Veterinary Research Institute, Khartoum, Sudan.
[07] Sahar M. E., Veterinary Research Institute, Khartoum, Sudan.
[08] Baraa A. M., Veterinary Research Institute, Khartoum, Sudan.
[09] Ahmed O. A., Veterinary Research Institute, Khartoum, Sudan.
[10] Ali Y. H., Veterinary Research Institute, Khartoum, Sudan.
Abstract
In June 2013, a severe respiratory disease with high morbidity 75% (75/100) and low mortality (2%) occurred at the Khartoum Horse Racing Stable (KHRS); nasal discharge “serous to mucopurulent”, dry hacking cough and pyrexia were the main clinical signs observed. The one animal subjected to Post mortem examination showed, congestion in lung and liver and myocarditis. During the course of the disease which lasted about one week. 20 out of 23 horses (87%) at Halfaya Stable (Khartoum north), showed clinical signs similar to that of Khartoum Stable, but none of the horses died. Equine influenza H3N8 virus was isolated from lung and nasal swab samples inoculated into allantoic cavity of 10 day embryonating chicken eggs. The virus was confirmed serologically by HA & HI tests and by one step RT-PCR. The results of the study confirmed the presence of influenza a virus in horses in Khartoum state as well as the isolation of the virus for the first time in the country from clinical cases.
Keywords
Equine Influenza, Clinical Sign, One - Step RT - PCR
References
[01] Algezoli, O. A and Kheir, S. A. M. 2014. Seroprevalence of Equine Influenza Virus in South Darfur State, Sudan. Sudan J. Vet. Res. 29: 39-42.
[02] Barbic, L., Madic, J., Turk, N. and Daly, J. 2009. Vaccine failure caused an outbreak of equine influenza in Croatia. Vet. Microb. 133: 164–171.
[03] Bates, T. W., Thurmond, M. C. and Carpenter, T. E. 2001. Direct and indirect contact rates among beef, dairy, goat, sheep, and swine herds in three California counties, with reference to control of potential foot-and-mouth disease transmission. Am. J. Vet. Res. 62: 1121-1129.
[04] Begg, A. P., Reece, R. L., Hum, S., Townsend, W., Gordon, A. and Carrick, J. 2011. Pathological changes in horses dying with equine influenza in Australia, 2007. Aust. Vet. J. 89 (Suppl 1): 19–22.
[05] Callinan, I. 2008. Equine Influenza. The August 2007 outbreak in Australia. http://www.equineinfluenzainquiry.gov.au/eiiexhibits/REP.0001.001.0001.pdf.
[06] Cowled, B., Ward, M. P., Hamilton, S. and Garner, G. 2009. The equine influenza epidemic in Australia: spatial and temporal descriptive analyses of a large propagating epidemic. Prev. Vet. Med. 92: 60–70.
[07] Dalglish, R. A. 1992. The international movement of horses — the current infectious disease situation. In: Proceedings of the 9th International Conference of Racing Analysts and Veterinarians, Short CR (ed), New Orleans, 1992, International Conference of Racing Analysts and Veterinarians and Louisiana State University, 37–53.
[08] Donofrio, J. C., Coonrod, J. D. And Chambers, T. M. 1994. Diagnosis of equine influenza by the polymerase chain reaction. J. Vet. Diagn. Investig. 6: 39–43.
[09] EI Epidemiology Support Group 2009. Equine influenza 2007: the Australian experience, Animal Health Australia, Canberra.
[10] Foord, A. J., Selleck, P., Colling, A., Klippel, J., Middleton, D., Heine, H. G., 2009. Real-time RT-PCR for detection of equine influenza and eval- uation using samples from horses infected with A/equine/Sydney/2007(H3N8). Vet. Microbiol. 137: 1–9.
[11] Fucchier, R. A. M., Bestebroer, T. M., Herfst, S., Van Der Kemp, L., Rimmelzwaan, G. F. and Osterhaus, A. D. M. E. 2000. Detection of influenza A viruses from different species by PCR amplification of conserved sequences in the matrix gene. J. clin. Microbiol. 38: 4096-4101.
[12] Glass, K., Wood, J. L., Mumford, J. A., Jesset, D. and Grenfell, B.T. 2002. Modelling equine influenza 1: a stochastic model of within-yard epidemics. Epidemiol and Infec. 128: 491–502.
[13] Guo, Y., Wang, M., Zheng, G. S., Li - k, W., Kawaoka, Y. and Webster, R. G. 1995. Seroepidemiological and molecular evidence for the presence of two H3N8 equine influenza viruses in China in 1993-94. J. Gen. Virol. 76: 2009-2014.
[14] Gupta, A. K., Yadav, M. P. and Uppal, P. K. 1993. Characterization of equine influenza isolates from the 1987 epizootic in India by nucleotide sequencing from the HA1 gene. Eq. Vet. J. 25: 99-102.
[15] Guthrie, A. J., Stevens, K. B. and Bosman, P. P. 1999. The circumstances surrounding the outbreak and spread of equine influenza in South Africa. Revue scientifique et technique (Office International des Epizooties) 18(1): 179–185.
[16] Happold, J. and Rubira, R. 2011. Equine influenza: Patterns of disease and seroprvalence in throughbred studs and implications of vaccination. Aust. Vet. J. 89: 135- 137.
[17] Ismail, T. M., Sami, A. M., Youssef, H. M. and Abou Zaid, A. A. 1990. An outbreak of equine influenza type 1 in Egypt in 1989. Vet. Med. J. Giza. 38: 195–206.
[18] Miller, W. C. 1965. Equine influenza: Further observations on the “Coughing” Outbreak. Vet. Rec. 77: 455–456.
[19] OIE, 2015. Manual of standards for diagnostics tests and vaccines, equine influenza. Chapter 2.5.7.
[20] Oxburgh, L. and Hagstrom. A. 1999. A PCR based method for the identification of equine influenza virus from clinical samples. Vet. Microbiol. 67: 161–174.
[21] Paillot, R., Hannant, D., Kydd, J. H. and Daly, J. M. 2006. Vaccination against equine influenza:Quid novi? Vaccine. 24: 4047-4061.
[22] Patterson-Kane, J. C., Carrick, J. B., Axon, J. E., Wilkie, I. and Begg, A. P. 2008. The pathology of bronchointerstitial pneumonia in young foals associated with the first outbreak of equine influenza in Australia. Eq. Vet. J. 40: 199–203.
[23] Rosanowski, S. M. 2012. Epidemiological investigations of the New Zealand horse population and the control of equine influenza PhD thesis. Massey university, Palmerston North, New Zealand.
[24] Satou, K. and Nishiura, H. 2006. Basic reproduction number for equine-2 influenza virus a (H3N8) epidemic in racehorse facilities in Japan, 1971. J. Equi. Vet. Sci., 26: 310-316.
[25] Sluyter, F. J. H. 2001. Traceability of equidae: A population in motion. OIE Revue Scientifique et Technique. 20: 500-509.
[26] Sovinova, O., Tumuva, B., Poustka, F. and Nemec, J. 1958. Isolation of virus causing respiratory disease in horses. Acta. virol. 2(1): 52-61.
[27] Spackman, E., Senne, D. A., Myers, T. J., Bulaga, L. L., Garber, L. P., Perdue, M. L., Lohman, K., Daum, L. T. and Suarez, D. L. 2002. Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. J. Clin. Microbiol. 40: 3256–3260.
[28] Van Maanen, C. and Cullinane, A. 2002. Equine influenza virus infections: an update. Vet. Q. 24: 79–94.
[29] Waddell, G. H., Teigland, M. B. and Sigel, M. M. 1963. A new influenza virus associated with equine respiratory disease. JAVMA. 143 (6): 587-559.
600 ATLANTIC AVE, BOSTON,
MA 02210, USA
+001-6179630233
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - American Institute of Science except certain content provided by third parties.