International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.2, No.1, Feb. 2016, Pub. Date: Feb. 17, 2016
Antioxidants and Hypoglycemic Studies on Egyptian Propolis and Foeniculum Vulgare on Alloxan Induced Diabetic Rats
Pages: 1-10 Views: 2500 Downloads: 1542
Authors
[01] Samir A. M. Zaahkouk, Department of Zoology, Faculty of Science, Al-Azhar University, Cairo, Egypt.
[02] Diaa F. Ibrahim, Department of Zoology, Faculty of Science, Al-Azhar University, Cairo, Egypt.
[03] Bassem E. Elarabi, Department of Zoology, Faculty of Science, Al-Azhar University, Cairo, Egypt.
Abstract
Background: Diabetes Mellitus (DM) is one of the leading causes of death, illness, and economic loss in the Egypt. In DM the oxidative stress exceeds the body’s antioxidant defense mechanisms. Although oxidative stress and free radicals have been reported to play a significant role in diabetic complications. Propolis and Foeniculum vulgare (fennel) possesses antioxidant and anti-diabetic activities. Aim of work: This study was carried out to evaluate the effect of Egyptian propolis and Foeniculum Vulgare on hyperglycemia-induced oxidative stress in diabetic rats. Materials and methods: Diabetes was induced with Alloxan (148mg/Kg) intraperitoneal. Rats with blood glucose level less than 300 mg/dL were excluded. Animals were divided into 8 equal groups (n=80); except negative control rats, all groups inoculated with alloxan Group 1: negative control of normal rats. Group 2: positive control of alloxan injected rats. Group 3: rats were treated with 200 mg/ kg of propolis. Group 4: rats were treated with 400 mg/ kg of propolis. Group 5: rats were treated with 200 mg/ kg of Foeniculum vulgare. Group 6: rats were treated with 400 mg/ kg of Foeniculum vulgare. Group 7: rats were treated with (200 mg/ kg of Foeniculum vulgare +200 mg/ kg of propolis). Group 8: rats were treated with (400 mg/ kg of Foeniculum vulgare +400 mg/ kg of propolis). Rats were treated orally for 28 days. Results: Data showed significant increase in serum levels of Fasting blood glucose FBG, malonaldehyde MDA, superoxide dismutase SOD and significant decreased levels of insulin, Catalase, glutathione (GSH) reduce, glutathione -S- transferase (GST) were observed in the diabetic untreated animals. Conclusion: Propolis and Foeniculum Vulgare possesses hypoglycemic activities in addition to its ability to ameliorate oxidative stress induced organ dysfunction.
Keywords
Diabetes, Propolis, Foeniculum Vulgare, Alloxan, Hyperglycemia, Oxidative Stress
References
[01] (A.D.A) American Diabetic Association, National Diabetes Statistics Fact Sheet, 2008, http://diabetes.niddk.nih.gov/dm/pubs/ statistics/index.htm.
[02] Abdul-Hadi A. H. (2014): Study the Effect of Iraqi Propolis Extract on Hematological Parameters in Alloxan-Induced Diabetic Rabbits. MRSVA. 3 (2), 1-10.
[03] Aebi, H. (1984): Catalase in vitro methods in enzymology (Ed.B. J. Willam). New Yourk, ABD 105: 121-126.
[04] Afshari, A.T., Shirpoor, A., Farshid, A., Saadatian, R., Rasmi, Y., Saboory, E., Ilkhanizadeh, B., Allameh, A. (2007): The effect of ginger on diabetic nephropathy, plasma antioxidant capacity and lipid peroxidation in rats. Food Chem; 101:148–153.
[05] Ahuja, V. and Ahuja, A. (2011): Apitherapy - A sweet approach to dental diseases. Part II: Propolis. Journal of Academy of Advanced Dental Research, Vol 2; Issue 2.
[06] Al-Hariri, M. (2011): Glycemic control and anti-osteopathic effect of propolis in diabetic rats. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy: 4. 377–384
[07] Amin A., Mustafa I. O., Folarin R. O., Onanuga I. O., Ibrahim R. B. and Balogun W.G. (2013): Effect of Nigerian propolis on glycemia, lipid profile, and oxidative stress markers in alloxan-induced diabetic rats. vol. 2. 149–158.
[08] Bahekar R. and Jain M. (2012): Exendin-4 activates glucokinase. J. of Diabetes; 4(4): 369-77.
[09] Baynes, J.W. (1991): Role of oxidative stress in development of complications in diabetes. Diabetes; 40(4): 405-12.
[10] Beutler, E., Duron, O., and Kelly, B. M. (1963): Improved method for the determination of blood glutathione. J. Lab. Clin. Med., 61(5): 882-888.
[11] Bhattacharyya, D., Pandit, S., Mukherjee, R., Das, N., and Sur, T. K. (2003): Hepatoprotective effect of Himoliv, a poly herbal formation in rats. Ind J Physiol Pharmaol 47(3): 435-440.
[12] Boddupalli A., Kumanan R., Elumalai A. (2012): A twelvemonthly review on anti-diabetic plants. Int Res J Pharmacy; 3(1): 77-80.
[13] Chen C., Weng M., Wu C., Lin J. (2004): Comparison of radical scavenging activity, cytotoxic effects and apoptosis induction in human melanoma cells by Taiwanese propolis from different sources. Complementary and Alternative Medicine; 1: 175–185.
[14] Dhanesha N., Joharapurkar A., Shah G., Dhote V., Kshirsagar S., Bahekar R., Jain M. (2012): Exendin-4 activates glucokinase. Journal of Diabetes; 4(4): 369-77.
[15] Dods, R. F. (2003): Diabetes Mellitus Clinical Chemistry: Theory Analysis, Correlation. 4th Edition, Kaplan, L. A., Pesce, A. J., and Kazmierczak, S. C. 580.
[16] Eastham, R.D. (1985): Biochemical values in clinical medicin.7th. Ed.Bristol England. John wright& sons, Ltd., P.110-120.
[17] El-Sayed El-SM., Abo-Salem O.M., Aly H.A., Mansour A.M. (2009): Potential antidiabetic and hypolipidemic effects of propolis extract in streptozotocin-induced diabetic rats. Pak J. Pharm Sci; 22(2): 168–174.
[18] El-Akabawy, L.M., Zayan, K.A., Tantawy, A.A, Omar, R.E.M., (2004): Anticoccidial efficacy of Propolis and Toltrazuril against Eimeria stiedae in New Zealand white rabbits. Zag. Vet. Med. J. (32) 129-145.
[19] Etuk E.U. (2010): Animals models for studying diabetes mellitus. Agriculture and Biology J. of North America; 1:130134.
[20] Giugliano D., Ceriello A., Paolisso G. (1996): Oxidative stress and diabetic vascular complications. Diabetes Care; 19(3): 257–267.
[21] Habig, W. H., Pabst, M. J., and Jacoby, W. B. (1974): Glutathione-S-transferase. The first enzymatic step in mercapturic acid formation. J. Bio. Chem., 249(22): 7130-7139.
[22] Halliwell, B. and John M. G. (1999): Free radicals in biology and medicine. Vol. 3. Oxford: Oxford university press.
[23] Halliwell, B. and Gutteridge, J.M. (1994): Lipid peroxidation, oxygen radicals, cell damage, and antioxidant therapy. Lancet; 1(8391): 1396–1397.
[24] Hamada, Y., Fujii, H., Kitazawa, R., Yodoi, J., Kitazawa, S., Fukagawa, M. (2009): Thioredoxin-1 overexpression in transgenic mice attenuates streptozotocin- induced diabetic osteopenia: a novel role of oxidative stress and therapeutic implications. Bone; 44(5): 936–941.
[25] Hanan F. A. and Nareman Y. M. (2015): The Possible Therapeutic Effects of Propolis on Osteoporosis in Diabetic Male Rats. Nature and Science 2015; 13(3) http: //www. sciencepub.net /nature.
[26] Hemieda, F. A., El-kholy, E., Wafaa, M.,El-sawah, Shady, G. (2015): Influence of propolis on oxidative stress, inflammation and apoptosis in streptozotocin induced diabetic rats. International Journal of Advanced Research, Vol. 3, Issue 7, 831-845.
[27] Koya-Miyata S., Arai N., Mizote A., Taniguchi Y., Ushio S., Iwaki K., Fukuda S. (2009): Propolis Prevents Diet-Induced hyperlipidemia and mitigates weight gain in Diet-Induced obesity in mice. Biol. Pharm. Bull; 32(12): 2022—2028.
[28] Lenzen, S. (2008): “The mechanisms of alloxan- and streptozotocininduced diabetes,” Diabetologia; 51(2): 216–226.
[29] Liu, Y. , Chen Z. , Liang Y., Cui L., Zheng D. , Guo W., Ren X., Wu Y. and Teng L.(2009): Extraction and Anti-diabetic Activity on Alloxan-induced Diabetic Mice of Polysaccharides from Huidouba. CHEM. RES. CHINESE UNIVERSITIES, 25(5), 681—685.
[30] Marklund, S., and Marklund, G. (1974): Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem., 47(3): 469-474.
[31] Matsui T, Ebuchi S, Fujise T. (2004): “Strong antihyperglycemic effects of water-soluble fraction of Brazilian propolis and its bioactive constituent, 3,4,5-tri-O-caffeoylquinic acid. Biol Pharm Bull; 27(11): 1797–1803.
[32] Mhaidat N.M., Abu-zaiton A. s., Alzoubi K. H., Alzoubi W. and Alazab R.S. (2015): Antihyperglycemic Properties of Foeniculum vulgare extract in Streptozocin-Induced Diabetes in Rats. International J. of Pharmacology 11 (1): 72-75.
[33] Mostafa, D. M., Abd El-Alim, S. H., Asfour, M. H., Al-Okbi, S. Y., Mohamed, D. A and Awad. G. (2015): Transdermal Nanoemulsions of Foeniculum vulgare Mill. Essential Oil: Preparation, Characterization and Evaluation of Antidiabetic Potential. J. of Drug Delivery Science and Technology. Vol. 29. P. 99–106.
[34] Orsolic N., Sirovina D., Gajski G., Garaj-Vrhovac V., Jazvinscak J.M., Kosalec I. (2013): Assessment of DNA damage and lipid peroxidation in diabetic mice: Effects of propolis and epigallocatechin gallate (EGCG).
[35] Oršolic N., Sirovina D., Koncic M. Z., Lackovic G. and Gregorovic G. (2012): Effect of Croatian propolis on diabetic nephropathy and liver toxicity in mice. BMC Complementary and Alternative Medicine, 12: 117.
[36] Osadebe P.O., Odoh E.U., Uzor P.F. (2014): The search for new hypoglycemic agents from plant. Afr. J. Pharm Pharmacol; 8(11): 292-303.
[37] Ramkumar, K.M., Ponmanickam, P., Velayuthaprabhu, S., Archu- nan, G., Rajaguru, P. (2009): “Protective effect of Gymnema montanum against renal damage in experimental diabetic rats. Food Chem. Toxicol., 47(10), 2516-2521.
[38] Sridhar, S. N. C., Kumari, S., Paul, A. T. (2014): Diabetic Complications: A Natural Product Perspective. Pharmaceutical Crops, 5, (Suppl 1: M4) 39-60.
[39] Szkudelski T. (2001): The mechanism of alloxan and streptozotocin action in β- cells of the rat pancreas.streptozotocin-induced diabetic rats. Maced. 1. Med. Sci., 4: 139-146.
[40] Yilmaz H.R., Uz E., Yucel N., Altuntas I., Ozcelik N. (2004): Protective effect of caffeic acid phenethyl ester (CAPE) on lipid peroxidation and antioxidant enzymes in diabetic rat liver. J. Biochem Mol Toxicol; 18(4): 234–238.
[41] Yoshioka, T., Kawada, K., Shimada, T., and Mori, M. (1979): Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am. J. Obstet. Gynecol., 135(3): 372-376.
[42] Zhu W., Chen M., Shou Q., Li Y., Hu F. (2010): Biological activities of Chinese Propolis and Brazilian Propolis on streptozotocin-Induced Type 1 Diabetes Mellitus in rats. ECAM; 1-8.
600 ATLANTIC AVE, BOSTON,
MA 02210, USA
+001-6179630233
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - American Institute of Science except certain content provided by third parties.