International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.3, No.3, Jun. 2017, Pub. Date: Nov. 1, 2017
Isolation and Molecular Characterization of E. coli, Salmonella spp. and Pasteurella spp. from Apparently Healthy Duck
Pages: 12-17 Views: 41 Downloads: 24
Authors
[01] Hasna Banu, ACI Animal Health, ACI Limited, Dhaka, Bangladesh.
[02] Md Zulfekar Ali, Animal Health Research Division, Bangladesh Livestock Research Institute (BLRI), Dhaka, Bangladesh.
[03] Sonia Akther, Goat and Sheep Production Research Division, Bangladesh Livestock Research Institute (BLRI), Dhaka, Bangladesh.
[04] Parbati Paul, Department of Livestock Services (DLS), Ministry of Fisheries and Livestock Khamarbari, Dhaka, Bangladesh.
[05] Md. Alimul Islam, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
[06] Mst. Minara Khatun, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Abstract
The present study was conducted for the isolation, identification and molecular detection of the bacterial flora present in the respiratory tract of apparently healthy 4 breeds (Khaki Campbell, Pekin, Jinding and Muscovy) of ducks. Out of 60, a total of 48 respiratory samples from Bangladesh Agricultural University poultry farm (BAUPF) and 12 from scavenge area were collected. Three bacteria were isolated and identified from samples by cultural, staining biochemical tests and molecular detection. The prevalence of E. coli, Salmonella spp. and Pasteurella spp. of Khaki Campbell duck were 50%, 41.67% &58.33% and 50%, 50% & 66.67% recorded from BAUPF and scavenging area, respectively. Out of 60 samples, the overall prevalence of E. coli, Salmonella spp. and Pasteurella spp. were 50%, 31.67% & 50%. In both sources, E. coli and Pasteurella spp. shown high prevalence in BAUPF and scavenging area. In BAUPF the isolation of E. coli, Slamonella spp. and Pasteurella spp. were 0, 2 & 2 and 2, 0 & 1from both adult and young Khaki Campbell duck. On the other hand, in scavenge area the isolation of E. coli, Slamonella spp. & Pasteurella spp. were 1, 2 & 2 and 2, 1 &1 from both adult and young Khaki Campbell duck. So it was found that the isolation of bacterial species shown higher in scavenge area than in BAUPF. The highest number of bacteria were recovered from lungs (27 isolates from 8 samples) while the lowest number were from tracheal washing and swab of infraorbital sinus (14 isolates from 8 sample in both cases). Total bacterial isolates were 6 isolates E. coli 3, Salmonella spp. 2 and Pasteurella spp. 2 were found positive by conventional PCR. The results of this study would be helpful for prevention and control of bacterial respiratory diseases in ducks.
Keywords
E. coli, Salmonella, Pasteurella, Duck, PCR
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