International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.4, No.1, Mar. 2018, Pub. Date: Jun. 14, 2018
Infestation of a Single Seed of Sapota Fruit by Seed Borer (Trymalitis Margaritas) Enhances Viability of Neighboring Seeds
Pages: 6-18 Views: 1290 Downloads: 629
Authors
[01] Seshadri Shivashankar, Division of Plant Physiology and Biochemistry, ICAR-Indian Institute of Horticultural Research, Bengaluru, India.
[02] Manoharan Sumathi, Division of Plant Physiology and Biochemistry, ICAR-Indian Institute of Horticultural Research, Bengaluru, India.
Abstract
Sapota fruit, Manilkara achras (Mill) Fosberg cv. Cricket ball shows high incidence of the physiological disorder known as Corky tissue under rainfed cultivation due to poor seed viability. Interestingly, however, fruits naturally infested by sapota seed borer (SSB), Trymalitis margaritas Meyrick (Tortricidae: Lepidoptera), remain free from the disorder. Hence, this study was carried out to elucidate the mechanism by which SSB infestation prevents corky tissue development in sapota. Comparative biochemical analyses of the uninfested seeds of SSB affected fruits and the seed of H and CT fruits showed that the activities of dehydrogenases, PEP carboxylase, concentrations of key metabolites such as, ATP, pyruvate, NAD and NADH and the contents of the growth hormones, ABA, IAA and GA were significantly higher in SSB seed showing that it was metabolically more active compared to seed from H and CT. The significantly higher rate and percentage of germination of uninfested seeds of SSB affected fruits confirmed higher seed viability and the markedly higher ratio of seed dry weight to fruit dry weight indicated higher sink strength and sink capacity compared to H and CT affected fruits. The study thus, confirmed that seeds from SSB affected fruit were more vigorous and viable compared to seed from CT infested and healthy fruits. The relatively lower levels of ROS and MDA coupled with lower electrical conductivity and higher pH reflected a higher level of tissue integrity of the SSB seed. Taken together, the study unequivocally established that infestation of a single seed by SSB led to enhanced seed vigour and viability in the neighbouring seeds and thus ensured that the infested sapota fruits remained free from corky tissue disorder. This novel finding could pave the way for prevention of incidence of corky tissue disorder in sapota by enhancement of seed viability during fruit growth.
Keywords
Corky Tissue, Dehydrogenases, Free Radicals, Sapota cv. Cricket Ball, Sapota Seed Borer, Seed Viability, TCA Cycle Intermediates
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