International Journal of Bioinformatics and Biomedical Engineering
Articles Information
International Journal of Bioinformatics and Biomedical Engineering, Vol.1, No.2, Sep. 2015, Pub. Date: Jul. 15, 2015
Analysis of Plum (Prunus domestica L.) Genotypes of Duhok City Using AFLP Markers
Pages: 64-69 Views: 984 Downloads: 909
Authors
[01] Shaymaa H. Ali, Scientific Research Center, Duhok University, Duhok, Iraq.
[02] Dalal Y. Sinjare, Scientific Research Center, Duhok University, Duhok, Iraq.
[03] Jaladet M. S. Jubrael, Scientific Research Center, Duhok University, Duhok, Iraq.
Abstract
A plum or gage is a stone fruit tree in the genus Prunus, subgenus Prunus. The subgenus is distinguished from other subgenera (peaches, cherries, bird cherries, etc) in the shoots having a terminal bud and the side buds solitary (not clustered).The samples were analyzed by using AFLP markers. Three primer combinations generated a total of 106 bands and among them86 were polymorphic (81.1%), while 20 (18.9%) were monomorphic. A relatively low genetic diversity has been observed by using AFLP. Genetic similarity values ranging from 0.2464 the lowest genetic distance found between achas yabani thahabi and achas bathengani, whereas the highest genetic distance 0.5415 between achas no.1 and achas maryana. Using UPGMA clustering analysis method based on the similarity coefficient, cultivars were separated into two major genetic clusters within first cluster there is two sub-groups consists of achas no.1, achas samari, achas aswad daymi, achas bathangani no.2 and achas yabani thahabi and a second cluster consist of achas maryana and achas bathangani. The results suggested that AFLP is a good method to determine genetic relatedness among plum genotypes in Duhok city.
Keywords
Prunus domestica L., AFLP, Genetic Diversity
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