International Journal of Bioinformatics and Biomedical Engineering
Articles Information
International Journal of Bioinformatics and Biomedical Engineering, Vol.1, No.2, Sep. 2015, Pub. Date: Sep. 9, 2015
Structural Analysis and 3D-Modeling of FANCD2 Proteins
Pages: 205-210 Views: 1282 Downloads: 357
Authors
[01] Abubaker H. Mohamed, Department of Bioinformatics, Africa City of Technology, Khartoum, Sudan.
[02] AbdAlla A. AbdAlla, Department of Biotechnology, Omdurman Islamic University, Omdurman, Sudan.
[03] Abdalbasit Mariod, Department of Biology, Faculty of Sciences and Arts-Alkamil, Jeddah University, Alkamil, Saudi Arabia;Food Science & Technology Department, College of Agricultural Studies, Sudan University of Science & Technology, Khartoum North, Sudan.
Abstract
Fanconi anemia (FA), is an uncommon inherited disease, is connected with dynamic bone marrow failure, inclination to cancer, and genomic instability. FA described by formative deformities, short stature, bone marrow failure, and a high danger of cancer. The mRNA of FANCD2 was recovered from the NCBI database, then interpreted into 679 amino acid by GeneMark server and study capacity of Fanconi anemia ID complex (FANCI-FANCD2) in light of the SWISSPORT Blast report: assumes a focal part in the repair of DNA interstrand cross-links, activated by means of DNA damage-induced phosphorylation by ATR and monoubiquitination by the FA center complex ubiquitin ligase, this capacity that permit processing and evacuation of crosslinked DNA and subsequently advances cell survival after DNA damage. The preservation homology was assessed by different arrangement utilizing BioEdit pakage, demonstrating high characters with another FANCD2 protein H. sapiens. The study structural and functional characterization, molecular formula, pI, EC, AI, GRAVY, instability index, half-life time and secondary structure were anticipated, this outcome affirm FANCD2 protein is steady, which can be utilized to confine and study in the vitro. In the present study a complete structural investigation and 3-D demonstrating of the FANCD2 protein utilized CPHmodels 3.2 server by utilizing template pdb of Mus musculus model because of the high rate of character (71.7%) and envision by Chimera 1.8. programming. Anticipated model was further surveyed by VERIFY-3D, this confirmation of quality of model.
Keywords
Fanconi Anemia, Protein, 3-D Modeling, GeneMark Server, SWISSPORT Blast Report, Structural and Functional Characterization
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