International Journal of Bioinformatics and Biomedical Engineering
Articles Information
International Journal of Bioinformatics and Biomedical Engineering, Vol.1, No.2, Sep. 2015, Pub. Date: Sep. 2, 2015
Bioinformatic Analysis of Phosphoglucomutase (PGM2) from Different Species of Plasmodia Using Computational Tools
Pages: 181-184 Views: 1205 Downloads: 329
Authors
[01] Nahla O. M. Ali, Department of Parasitology, Faculty of Veterinary Medicine, University of Khartoum, Khartoum, Sudan.
Abstract
In this study, PGM2 enzyme from different malaria parasite Plasmodium species was analyzed and presented in this communication. The composition of leucine, lysine and asparagines were the highest while lowest concentrations of tryptophan and histidine residues were noticed when compared to other amino acids. The pI value of P. vinckei PGM2 was 8.59 while the lowest pI of 5.91 was shown by P. vivax PGM2. The instability index of all the enzymes is variable, but for all of them it was less than 40, which indicates that all of them are stable. The aliphatic index was found to span within a range of 83 to 86. Secondary structural analysis of the enzymes showed the pre-dominance of Alpha helix followed by random coils for all the mutases except P. vivax, P. inui, P. Knowlesi and P. Fragile PGM2 enzyme. The significance of the above results is discussed in the light of existing literature.
Keywords
PGM2, Phosphoglucomutase, Plasmodium spp., Bioinformatics, Secondary Structure
References
[01] Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J. Mol. Biol. 1990 Oct 5; 215(3): 403-10.
[02] Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25: 3389-402.
[03] Azmi K, Schonian G, Schnur LF, Nasereddin A, Ereqat S, Abdeen Z. Development of assays using hexokinase and phosphoglucomutase gene sequences that distinguish strains of Leishmania tropica from different zymodemes and microsatellite clusters and their application to Palestinian foci of cutaneous leishmaniasis. PLoS Negl Trop Dis. 2013 Sep 26; 7(9): e2464.
[04] Bahl A, Brunk B, Coppel RL, Crabtree J, Diskin SJ, Fraunholz MJ, Grant GR, Gupta D, Huestis RL, Kissinger JC, Labo P, Li L, McWeeney SK, Milgram AJ, Roos DS, Schug J, Jr. Stoeckert CJ. Plasmo DB: the Plasmodium genome resource. An integrated database providing tools for accessing, analyzing and mapping expression and sequence data (both finished and unfinished). Nucleic Acids Res. 2002 J an1; 30(1): 87-90.
[05] Bairoch A and Apweiler R. "The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000" Nucl. Acids Res. 2000; 28: 45–48.
[06] Bandini G, Mariño K, Güther ML, Wernimont AK, Kuettel S, Qiu W, Afzal S, Kelner A, Hui R, Ferguson MA. Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase. Mol. Microbiol. 2012 Aug; 85(3): 513-34.
[07] Bayoumi RA, Bashir AH, Abdulhadi NH. Resistance to falciparum malaria among adults in central Sudan. Am. J. Trop. Med. Hyg. 1986 Jan; 35(1): 45-55.
[08] Berninsome PM and Hirscherg CB. Nucleotide sugar transporters of the Golgi apparatus. Curr. Opin. Struct. Biol. 2000 Oct; 10(5): 542-547.
[09] Brown DH. "Glycogen metabolism and glycolysis in muscle". Myology 1986. NewYork: McGraw-Hill. pp. 673–95.
[10] Dai JB, Liu Y, Jr.Ray WJ, Konno M. The crystal structure of muscle phosphoglucomutase refined at 2.7-angstrom resolution. J. Biol. Chem. 1992 March 26; 267(9): 6322-6337.
[11] Gasteiger E, Jung E, Bairoch A. "SWISS-PROT: Connecting biological knowledge via a protein database" Curr. Issues Mol. Biol. 2001 Jul; 3(3): 47–55.
[12] Geourjon C and Deleage G. "SOPMA: Significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments" Comput. Appl. Biosci. 1995 Dec; 11(6): 681–684.
[13] Gomi M, Sonoyama M, Mitaku S. High performance system for signal peptide prediction: SOSUI signal Chem-Bio. Info. J., 2004, 4: 142-147.
[14] Hirschberg CB, Robbins PW, Abeijon C. Transporters of nucleotide sugars, ATP, and nucleotide sulphate in the endoplasmic reticulum and Golgi apparatus. Annu. Rev. Biochem. 1998; 67: 49-69.
[15] Lian LY, Al-Helal M, Roslaini AM, Fisher N, Bray PG, Ward SA, Biagini GA. Glycerol: an unexpected major metabolite of energy metabolism by the human malaria parasite. Malar. J. 2009 Mar 6; 8: 38.
[16] Mithieux G, Ajzannay A, Minassian C. Identification of membrane-bound phosphoglucomutase and glucose-6 phosphatase by 32P-labeling of ratlive rmicrosomal membrane proteins with 32P-glucose-6 phosphate. J. Biochem. 1995 Apr; 117(4): 908-14.
[17] Najjar VA, and Pullman ME. "The Occurrence of a Group Transfer Involving Enzyme (phosphoglucomutase) and Substrate". Science 1954 May 7; 119(3097): 631–4.
[18] Olliaro PL and Yuthavong Y. An overview of chemotherapeutic targets for antimalarial drug discovery. Pharmacol. Ther. 1999 Feb; 81(2): 91–110.
[19] Pagni M, Ioannidis V, Cerutti L, Zahn-Zabal M, Jongeneel C, Jo¨rg H, Olivier M, Dmitri K, Laurent F. "My Hits: Improvements to an interactive resource for analyzing protein sequences" Nucleic Acids Res. 2007; 35: W433–W437.
[20] Penha LL, Sant'Anna CB, Mendonça-Previato L, Cunha-e-Silva NL, Previato JO, Lima AP. Sorting of phosphoglucomutase to glycosomes in Trypanosoma cruzi is mediated by an internal domain. Glycobiology. 2009 Dec; 19(12): 1462-72.
[21] Rhyu GI, Ray Jr, William M, John L. "Enzyme-bound intermediates in the conversion of glucose 1-phosphate to glucose 6-phosphate by phosphoglucomutase. Phosphorus NMR studies". Biochemistry 1984, 23(2): 252–60.
[22] Whitehouse DB, Tomkins J, Lovegrove JU, Hopkinson DA, McMillan WO. A phylogenetic approach to the identification of phosphoglucomutase genes. Mol. Biol. Evol. 1998 Apr; 15(4): 456-62.
600 ATLANTIC AVE, BOSTON,
MA 02210, USA
+001-6179630233
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - 2017 American Institute of Science except certain content provided by third parties.