International Journal of Bioinformatics and Biomedical Engineering
Articles Information
International Journal of Bioinformatics and Biomedical Engineering, Vol.2, No.1, Jan. 2016, Pub. Date: Jan. 9, 2016
Molecular Identification of a cDNA Encoding the Hypoxanthine-Guaninephosphoribosyltransferase (HGPRT) from Rodent Malaria Strain of Plasmodium berghei
Pages: 8-14 Views: 778 Downloads: 439
Authors
[01] Nahla O. M. Ali dr_nahla2004@yahoo.com, Department of Parasitology, Faculty of Veterinary Medicine, University of Khartoum, Khartoum, Sudan;Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India.
Abstract
The hypoxanthine-guaninephosphoribosyltransferase (HGPRT) enzyme of Plasmodium berghei, plays a key role in the salvage of preformed purine nucleotides from parasite-infected erythrocytes. Since P. berghei cannot synthesize purines de novo, development of inhibitors specific for the parasite HGPRT should be an effective method of chemotherapy. This gene has been annotated as encoding PRTase activity and proposed as essential for survival of the P. berghei. The aim of this work is to identify the HGPRT from P. berghei using bioinformatic tools in searching the FullMal database for EST sequences for a cDNA clone with full length HGPRT, design specific oligonucleotides primers and then isolate PbHGPRT from the cDNA clone. The PCR analysis produced an amplicon of 696 bp which is in agreement with the predicted size from the nucleotides sequence deduced from the HGPRT cDNA open reading frame. The significance of the above results is discussed in the light of existing literature.
Keywords
Plasmodium berghei, HGPRT, Phosphoribosyltransferase, Bioinformatics, EST, cDNA
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