International Journal of Biomedical and Clinical Sciences
Articles Information
International Journal of Biomedical and Clinical Sciences, Vol.3, No.2, May 2018, Pub. Date: Apr. 9, 2018
Comparison of Yield and Phytoconstituents of Vernonia amygdalina and Ocimum gratissimum Leaves Extract from Three Extraction Methods
Pages: 27-34 Views: 1456 Downloads: 514
[01] Stanley Irobekhian Reuben Okoduwa, Directorate of Research and Development, Nigerian Institute of Leather and Science Technology, Zaria, Nigeria.
[02] Oche Okpe, Department of Biochemistry, Federal University of Agriculture, Makurdi, Nigeria.
[03] Ugochi Judith Okoduwa, Petrochemicals and Allied Department, National Research Institute for Chemical Technology, Zaria, Nigeria.
[04] Bernard Egwu Igiri, Directorate of Research and Development, Nigerian Institute of Leather and Science Technology, Zaria, Nigeria.
[05] Daniel Hassan Mhya, Department of Biochemistry, College of Medical Sciences, Abubakar Tafawa Balewa University, Bauchi, Nigeria.
[06] Lovina Okon Mbora, Directorate of Science Laboratory Technology, Nigerian Institute of Leather and Science Technology, Zaria, Nigeria.
The economical extraction of large amount of specific phytoconstituents with a smaller quantity of solvent requires a simple and safe technique, since extraction is one of the most significant stages in the discovery of new active drug-candidate. This study investigated the phytochemical contents of two medicinal plants, extracted using different extraction methods. Twenty grams of Vernonia amygdalina (VA) and Ocimum gratissimum (OG) leaves were extracted with three different extraction methods (maceration, Soxhlet and microwave) using methanol. The yield and phytochemical contents were compared. The results showed that the percentage recovery yield of phytochemical content was significantly (p<0.05) higher in the extracts obtained from microwave method (20.55%), when compared to the Soxhlet (18.55%) and maceration technique (15.60%). The extracts obtained from microwave extraction protocol had significantly (p<0.05) higher concentrations in phenolics, saponins and tannins. Soxhlet extraction gave extract with significantly (p<0.05) higher yield on alkaloid, while the extract from maceration was higher in flavonoids. This study showed that the concentration of specific phytoconstituents obtained from medicinal plants depends on the extraction method. The results from this study suggest that Soxhlet technique is best for extraction of alkaloid, maceration technique for flavonoids and microwave assisted extraction technique for phenolics, saponins and tannins.
Medicinal Plants, Phytochemical, Vernonia amygdalina, Ocimum gratissimum, Microwave Extraction, Soxhlet, Maceration
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