International Journal of Biomedical and Clinical Sciences
Articles Information
International Journal of Biomedical and Clinical Sciences, Vol.4, No.1, Mar. 2019, Pub. Date: Mar. 19, 2019
Effects of Medicinal Plants on Acetylcholinesterase Activity in the Blood of Mice Infected with Plasmodium berghei
Pages: 11-16 Views: 1269 Downloads: 340
Authors
[01] Okpe Oche, Department of Biochemistry, Federal University of Agriculture, Makurdi, Nigeria.
[02] Upev Vincent Aondohemba, Department of Biochemistry, Federal University of Agriculture, Makurdi, Nigeria.
[03] Eimonye Jack Onmoiji, Department of Chemical Pathology, Benue State University, Makurdi, Nigeria.
[04] Iekaa Mimidoo Paula, Department of Biochemistry, Federal University of Agriculture, Makurdi, Nigeria.
[05] Enoyi Cecilia Oowole, Department of Biochemistry, Federal University of Agriculture, Makurdi, Nigeria.
[06] Josiah Silas Ojor, Department of Biochemistry, Federal University of Agriculture, Makurdi, Nigeria.
Abstract
Plasmodium species and acetylcholinesterase (AChE) is implicated in cerebral malaria, which is the cause of 12% of psychiatry disorder and a leading cause of death in sub-Saharan Africa. In this study we examined the AChE activity in the blood of mice infected with plasmodium berghei (P. berghei) and treated with leaf extracts of T. occidentalis, V. doniana, V. amygdalina, O. basilicum and Artesunate respectively. The parasitemia level and PCV evaluated; and the AChE activity in each mouse were determined using acetylthiocholine as substrate. There was significant (p˂ 0.05) increase in AChE activity in the extracts groups (0.0491-0.1573, 0.0431-0.2061, 0.0327-0.2031, 0.0513-0.0992 × 10-3 µmol ACTC min-1mg protein-1 respectively) and the standard drug (0.044-0.147 × 10-3 µmol ACTC min-1mg protein-1) of mice infected and treated for 7 days compared with the Normal and Disease control groups (0.3898-0.3898 and 0.156-0.0888 × 10-3 µmol ACTC min-1mg protein-1 respectively). The parasitemia level in the disease control group increases with days, which significantly (p˂ 0.05) reduces in groups that received plant extracts and Artesunate treatment. The PCV was stabilized in the treatment groups after an initial reduction. This finding reveals that the blood AChE activity decreases during infection of P. berghei, which was reversed upon administration of plant extracts and Artesunate. The increase in enzyme activity inversely correlates with reduction in parasite load. The findings have major implications for understanding how the plant extracts interact to enhance resistance to P. berghei proliferation in the body system.
Keywords
Plasmodium berghei, Acetylcholinesterase, Parasitemia, Plant Extracts, Artesunate
References
[01] Ibrahim HN, Imam IA, Bello AM, Umar U, Muhammad S, Abdullahi SA. The potential of Nigerian medicinal plants as antimalarial agent. Inter J Sci Tech 2012; 2 (8): 600-605.
[02] Linares M, Postigo M, Cuadrado D, Ortiz‑Ruiz A, Gil‑Casanova S, et al. Collaborative intelligence and gamification for on-line malaria species differentiation. Malar J 2019; 18 (21): 1-9.
[03] Wolfgang WL, Elke SB, Evelina A. Comparison of plasmodium berghei challenge models for evaluation of pre-erythrocytic malaria vaccines and their effect on perceived vaccine efficacy. Malaria J 2010; 9 (145): 1-12.
[04] Habluetzel A, Pinto B, Tapanelli S, Nkouangang J, Saviozzi M, et al. Efects of Azadirachta indica seed kernel extracts on early erythrocytic schizogony of Plasmodium berghei and pro-infammatory response in inbred mice. Malar J 2019; 18 (35): 1-9.
[05] Isma’il S, Amlabu E, Andrew JN. Antimalaria effect of the ethanolic stem bark extracts of Ficus platyphylla Del. J Parasitol Res 2011; Article ID 618209, 5 pages.
[06] Nyamai DW, Tastan OB. Aminoacyl tRNA synthetases as malarial drug targets: a comparative bioinformatics study. Malar J 2019; 18 (13): 1-27.
[07] Mark FW, Patricia AW, John WE, Sheppard JR. Membrane-associated phosphoproteins in plasmodium berghei-infected Murine erythrocytes. The J Cell Biol 1988; 97: 196-201.
[08] Ronan J, Fatima E, Valery C. Georges EG. In-vitro culture of plasmodium berghei-ANKA maintains infectility of mouse erythrocytes inducing cerebral malaria. Malaria J 2011; 10 (346): 1-5.
[09] Zatta P, Ibn-Lkhayat-Idrissi M, Zambenedetti P, Kilyen M, Kiss T. In vivo and in vitro effects of aluminum on the activity of mouse brain acetylcholinesterase. Brain Res Bul 2002; 59 (1): 41-45.
[10] Rajasree PH, Ranjit S, Sankar C. Screening for acetylcholinesterase inhibitory activity of methanolic extract of Cassia fistula roots. Inter J Pharm Life Scie 2012; 3 (9): 1976-1978.
[11] Inestrosa NC, Alvarez A, Perez CA, Moreno RD, Vicente M, Linker C, Casanueva OI, Soto C, Garrido J. Acetylcholinesterase accelerates assembly of amyloid-β-peptides into Alzheimer’s fibrils: possible role of the peripheral site of the enzyme. Neuron 1996; (16): 881-891.
[12] Inestrosa NC, Dinamarca MC. Alvarez A. Amyloid-cholinesterase interactions. Implications for Alzheimer’s disease. FEBS J 2008; 275: 625-632.
[13] Rees T, Hammond PI, Soreq H, Younkin S, Brimijoin S. Acetylcholinesterase promotes beta-amyloid plaques in cerebral cortex. Neurobiol Aging 2003; 24: 777-787.
[14] Rees TM, Berson A, Sklan EH, Younkin L, Younkin S, Brimijoin S, Soreq H. Memory deficits correlating with acetylcholinesterase splice shift and amyloid burden in doubly transgenic mice. Curr Alzheimer Res 2005; 2: 291-300.
[15] Srikumar BN, Ramkumar K, Raju TR, Shankaranarayana R. Assay of acetylcholinesterase activity in the brain. Brain Behav 2004; 142-144.
[16] Shin-Hua L, Josephine WW, Hsuan-Liang L, Jian-Hua Z, Kung-Tien L, Chih-Kuang C, Hsin-Yi L, Wei-Bor T, Yih H. The discovery of potential acetylcholinesterase inhibitors: A combination of pharmacophore modeling, virtual screening, and molecular docking studies. J Biomed Scie 2011; 18 (8): 1-13.
[17] Artitaya T, Phunuch M, Wanna C, Kesara N, et al. Antimalarial Activity of Piperine. J Trop Med 2018: 1-7.
[18] World Health Organisation. Legal status of traditional medicines and complementary alternative medicine: A worldwide review, 2011.
[19] Elujoba AA, Odeleye OM, Ogunyemi CM. Traditional medicine development for medical and dental primary health care delivery system in Africa. Afri J Trad Alter Med 2005; 2: 46-61.
[20] Fidock DA, Rosenthal PJ, Croft SL, Brun R, Nwaka S. Antimalarial drug discovery: efficacy models for compound screening. Nat Rev Drug Discov 2004; 3 (6): 509-520.
[21] Srikumar BN, Ramkumar K, Raju TR, Shankaranarayana R. Assay of acetylcholinesterase activity in the brain. Brain Behav 2004: 142-144.
[22] Bradford MM. A rapid sensitive method for quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal Biochem 1976; (72): 248-254.
[23] Duncan D. Multiple Range and Multiple F Tests. Biomet 1955; 11: 1-42.
[24] Tripathi A, Srivastava UC. Acetylcholineaterase, a versatile enzyme of the nervous system. Annals Neuroscie 2008; 15: 106-110.
[25] Ajagbonna OP, Esaigun PE, Alayande NO, Akinloye AO. Anti-malarial activity and haematological effect of stem bark water extract of Nuclea latifolia. Bioscie Res Comm 2002; 14 (5): 481-486.
[26] Brown BA. Hematology principles and procedures. 2nd Edition. Philadelphia, Lea and Febiger, 1976; pp. 200.
[27] Noor NA. Anxiolytic action and safety of Kava: Effect on rat brain acetylcholineaterase activity and some serum biochemical parameters. Afr J Pharm 2010; 4: 823-828.
[28] Aikawa M, Iseki M, Barnwell JW, Taylor D, Howard RJ. The pathology of human cerebral malaria. Ame J Med Hyg 1990; 43 (2): 30-37.
[29] Kamei K, Matsuoka H, Furuhata SI. Anti-malarial activity of leaf-extract of Hydrangea macrophylla, a common Japanese plant. Acta Medica Okayama 2000; 54 (5): 227-232.
[30] Dondorp AM, Day NP. The treatment of severe malaria. Trans R. Soc Trop Med Hyg 2007; 101 (7): 633-634.
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