International Journal of Chemical and Biomolecular Science
Articles Information
International Journal of Chemical and Biomolecular Science, Vol.1, No.2, Aug. 2015, Pub. Date: Jul. 21, 2015
Phytochemical Analysis of Gongronema latifolium Benth Leaf Using Gas Chromatographic Flame Ionization Detector
Pages: 60-68 Views: 1674 Downloads: 4816
[01] Chinedu Imo, Department of Biochemistry, Abia State University, Uturu, Nigeria.
[02] Friday O. Uhegbu, Department of Biochemistry, Abia State University, Uturu, Nigeria.
The aim of this study is to analyze the phytochemicals present in the leaf of Gongronema latifolium (Benth). The leaf of Gongronema latifolium (Benth) was harvested and sun-dried. The dried leaf was milled to fine powder. The milled leaf powder was weighed and used for the phytochemical analysis. Gas chromatographic flame ionization detector was used to quantify the phytochemicals analyzed. The results show the presence of different types of Alkaloids, Flavonoids, Total phenolic compound, Lignan, Terpenes, Sterol, Allicin, Hydroxycinnamic acids, Saponin and Carotinoid. Some of the phytochemicals detected in high quantities among the different groups of phytochemicals analyzed in 100g of Gongronema latifolium (Benth) leaf include Cinchonidine 52.47mg, Oxoassoanine 43.51mg, Lupanine 35.65mg and Buphanidrine 33.33mg (Alkaloids), Hyperoside 37.54mg, Quercetin 31.03mg and Kaemferol 24.80mg (Flavonoids), Tannic acid 116.60mg, Ferulic acid 82.26mg and Vanillic acid 64.17mg (Total phenolic compound), Retusin 4.40mg and Galgravin 4.33mg (Lignan), Nerol (geraniol) 33.05mg and Beta pinene 32.79mg (Terpenes), 5-avenasterol 9.42mg and Stigmasterol 4.89mg (Sterol), Chlorogenic acid 48.87mg and Caffeic acid 23.01mg (Hydroxycinnamic acids), Saponine 59.11mg and Sapogenin 50.79mg (Saponin) and Beta-crytoxanthin 433.14mg, Xanthophylls 311.36mg and Carotene 158.36mg (Carotenoid). The result shows that Gongronema latifolium (Benth) leaf possess an appreciable level of phytochemicals. It could be a good raw material for the production of some medicinal drugs and can be used in folk medicine for the treatment of some diseases.
Phytochemicals, Medicine, Gongronema latifolium Benth, Diseases
[01] Ugochukwu NH, Babady NE. Antioxidant effects of Gongronema latifolium in hepatocytes of rat models of non-insulin dependent diabetes mellitus. Fitoterapia, 2002;73:612-618.
[02] Ugochukwu NH, Babady NE, Cobourne M, Gasset SR. The effect of Gongronema latifolium extracts on serum lipid profile and oxidative stress in hepatocytes of diabetic rats. J. Biosci., 2003;20(1):1-5.
[03] Chinedu I, Uhegbu FO, Imo CK, Ifeanacho NG. Ameliorating effect and haematological activities of methanolic leaf extract of Gongronema latifolium in acetaminophen-induced hepatic toxicity in wistar albino rats. International Journal of Biosciences, 2013;3(11):183-188.
[04] Nwanjo HU, Okafor MC, Oze GO. Anti-lipid peroxidative activity of Gongronema latifolium in streptozotocin induced diabetes rats. Niger. J. Physiol. Sci. 2006;221(2):61-65.
[05] Egbung GE, Atangwho IJ, Iwara IA, Eyong UE. Micronutrient and phyto-chemical composition of root bark and twig extracts of Gongronema latifolium. Journal of Medicine and Medical Sciences, 2011;2(11):1185-1188.
[06] Tiwari AK, Roa M. Diabetes mellitus and multiple therapeutic approaches of phytochemical. Present status and future prospects. Current Science, 2002;83:30-38.
[07] Ugochukwu NH, Babady NE. Antihyperglycaemic of effect aqueous and ethanolic extracts of Gongronema latifolium leaves on glucose andglycogen metabolism in livers of normal and streptozotocin induced diabetic rats. Life Sci., 2003;73(150):1925-1938.
[08] Nwanjo HU, Alumanah EO. Effect of aqueous extract of Gongronema latifolium leaf on some indices of liver function in rats. Global J. Med. Sci., 2005;4(1):29-32.
[09] Imo C, Friday OU, Ifeanacho NG, Egbeigwe O, Ezekwe AS. Biochemical and histological changes associated with methanolic leaf extract of Gongronema latifolium in acetaminophen-induced hepatic toxicity in wistar albino rats. International Journal of Biomolecules and Biomedicine, 2014;4(2):1-7.
[10] Tripoli E, Guardia ML, Giammanco S, Majo DD, Giammanco M. Citrus flavonoids: Molecular structure, biological activity and nutritional properties: A review. Food Chemistry, 2007;104:466-479.
[11] Cora JD, Bruce GJ. Review Phytochemicals: nutraceuticals and human health. Journal of the Science of Food and Agriculture, 2000;80: 1744-1756.
[12] Manas KM, Pratyusha B, Debjani N. Phytochemicals – biomolecules for prevention and treatment of human diseases-a review. International Journal of Scientific and Engineering Research, 2012;3(7):1-32.
[13] Association of official Analytical Chemist. Official method of analysis. Washington DC, USA, 2009.
[14] Chehregani AH, Sabounchi SJ, Jodian V, Pak J. Antibacterial effect of N-NaphtylenDiamine Platinum (II) choloride as novel compound. Journal of Biological Sciences, 2007; 10(4): 641-644.
[15] Takagi S. Determination of green leaf carotenoids by HPLC. Agric Bio Chem., 1985; 49: 1211-1213.
[16] Kittakoop P, Mahidol C, Ruchirawat S. Alkaloids as important scaffolds in therapeutic drugs for the treatments of cancer, tuberculosis, and smoking cessation. Curr Top Med Chem., 2014;14 (2): 239–252.
[17] Russo P, Frustaci A, Del Bufalo A, Fini M, Cesario A. Multitarget drugs of plants origin acting on Alzheimer's disease. Curr Med Chem., 2013;20(13): 1686–1693.
[18] Raymond SS, Jonathan SJ, Michael WJ. The Essence of Analgesia and Analgesics. Cambridge University Press. 2010;pp.82–90.
[19] Cushnie T, Cushnie B, Lamb A. Alkaloids: An overview of their antibacterial, antibiotic-enhancing and antivirulence activities. Int J Antimicrob Agents, 2014;44(5):377–386.
[20] Qiu S, Sun H, Zhang A, Xu H, Yan G, Han Y. Natural alkaloids: basic aspects, biological roles, and future perspectives. Chin J Nat Med., 2014;12(6):401–406.
[21] Robbers JE, Speedie MK, Tyler VE. Alkaloids. Pharmacognosy and Pharmacobiotechnology. Philadelphia: Lippincott, Williams & Wilkins. 1996;pp.143-185
[22] Tsuchiya H. Structure-dependent membrane interaction of flavonoids associated with their bioactivity. Food Chemistry, 2010;120:1089-1096.
[23] Cook NC, Samman S. (1996). Flavonoids: Chemistry, metabolism, cardioprotective effects and dietary sources. Nutritional Biochemistry, 1996;7:66-76.
[24] Narayana KR, Reddy SR, Chaluvadi MR, Krishna DR. Bioflavonoids classification, pharmacological, biochemical effects and therapeutic potential. Indian Journal of Pharmacology, 2001;33:2-16.
[25] Tapas AR, Sakarkar D, Kakde RB. Flavonoids as nutraceuticals: A Review. Tropical Journal of Pharmaceutical Research, 2008;7:1089-1099.
[26] Ibtissem B. Antioxidant and antibacterial properties of Mesembryanthemum crystallinum and Carpobrotus edulis extracts. Advances in Chemical Engineering and Science, 2012;2(3):359.
[27] de Beer D, Joubert E, Gelderblom WCA, Manley M. Phenolic Compounds: A Review of Their Possible Role as In Vivo Antioxidants. South African Journal for Enology and Veticulture, 2002;23:48-61.
[28] Chung K, Wong TY, Wei C, Huang Y, Lin, Y. Tannins and Human Health: A Review. Critical Reviews in Food Science and Nutrition, 1998;38 (6): 421–464.
[29] Bouftira I, Chedly A, Souad S. Antioxidant and Antibacterial Properties of Mesembryanthemum crystallinum and Carpobrotus edulis Extracts. Advances in Chemical Engineering and Science, 2012;2(3):359-365.
[30] Gelinas P, McKinnon CM. Effect of wheat variety, farming site, and bread-baking on total phenolics. International Journal of Food Science and Technology, 2006;41(3):329.
[31] Beejmohun V, Fliniaux O. Microwave-assisted extraction of the main phenolic compounds in flaxseed. Phytochemical Analysis, 2007;18(4):275–285.
[32] Zory Q, Byung-Kee B. Phenolic Compounds of Barley Grain and Their Implication in Food Product Discoloration. J. Agric. Food Chem., 2006;54(26):9978–9984.
[33] Korkina L, Kostyuk V, De Luca C, Pastore S. Plant phenylpropanoids as emerging anti-inflammatory agents. Mini reviews in medicinal chemistry, 2011;11(10):823–835.
[34] Adlercreutz H. Lignans and human health. Critical reviews in clinical laboratory sciences, 2007;44 (5–6):483–525.
[35] Bergman JM, Thompson LU, Dabrosin C. Flaxseed and its lignans inhibit estradiol-induced growth, angiogenesis, and secretion of vascular endothelial growth factor in human breast cancer xenografts in vivo. Clinical cancer research: an official journal of the American Association for Cancer Research, 2007;13 (3):1061–1067.
[36] Lindahl G, Saarinen N, Abrahamsson A, Dabrosin C. Tamoxifen, flaxseed, and the lignan enterolactone increase stroma- and cancer cell-derived IL-1Ra and decrease tumor angiogenesis in estrogen-dependent breast cancer. Cancer research, 2011;71(1):51–60.
[37] Thimmappa R, Geisler K, Louveau T, O’Maille P, Osbourn A. Triterpene biosynthesis in plants. Annu Rev Plant Biol., 2014;65:225–257.
[38] Martin DM, Gershenzon J, Bohlmann J. Induction of Volatile Terpene Biosynthesis and Diurnal Emission by Methyl Jasmonate in Foliage of Norway Spruce. Plant Physiology, 2003;132(3):1586–1599.
[39] Pichersky E. Biosynthesis of Plant Volatiles: Nature's Diversity and Ingenuity. Science, 2006;311(5762):808–811.
[40] Russo EB. Taming THC: potential cannabis synergy and phytocannabinoid-terpenoid entourage effects. British Journal of Pharmacology, 2011;163(7):1344-1364.
[41] Katan MB, Grundy SM, Jones P, Law M, Miettinen TA, Paoletti R. Efficacy and safety of plant stanols and sterols in the management of blood cholesterol levels. Mayo Clin Proc, 2003;78:965-978.
[42] Nikolic V, Stankovic M, Nikolic LJ, Cvetkovic D. Mechanism and kinetics of synthesis of allicin. Pharmazie, 2004;59(1):10–14.
[43] Rabinkov A, Miron T, Konstantinovski L, Wilchek M, Mirelman D, Weiner L. The mode of action of allicin: trapping of radicals and interaction with thiol containing proteins. Biochim Biophys Acta, 1998;1379 (2):233–244.
[44] Abramovitz G, Gavri S, Harats D, Levkovitz H, Mirelman D, Miron T. et al. Allicin-induced decrease in formation of fatty streaks (atherosclerosis) in mice fed a cholesterol-rich diet. Coron. Artery Dis., 1999;10 (7):515–519.
[45] Elkayam A, Mirelman D, Peleg E, Wilchek M, Miron T, Rabinkov A. et al. The effects of allicin on weight in fructose-induced hyperinsulinemic, hyperlipidemic, hypertensive rats. Am. J. Hypertens, 2003;16(12):1053–1056.
[46] Lindsey JM, Bernhard HG, Veena V, Michael B, Samer RE, SunWook H. et al. The pungency of garlic: Activation of TRPA1 and TRPV1 in response to allicin. Current Biology, 2005;15(10):929–934.
[47] José T, Alexandra G, Manuela GE, Jorge G, Fernanda B. Hydroxycinnamic Acid Antioxidants: An Electrochemical Overview. BioMed Research International, 2013;2013:1-11.
[48] Onakpoya IJ, Spencer EA, Thompson MJ, Heneghan CJ. The effect of chlorogenic acid on blood pressure: a systematic review and meta-analysis of randomized clinical trials. Journal of Human Hypertension, 2015;29(2):77-81.
[49] Vincken JP, Heng L, Groot AD, Gruppen H. Saponins, classification and occurrence in the plant kingdom. Phytochem., 2007;68:275-297.
[50] Haralampidis, K., Trojanowska, M. and Osbourn, A. Biosynthesis of triterpenoid saponins in plants. Adv. Biochem. Eng. Biotechnol., 2002;75:31-49.
[51] Tadros MM, Ghaly NS, Moharib MN. Molluscicidal and schistosomicidal activities of a steroidal saponin containing fraction from Dracaena fragrans (L.). J Egypt Soc Parasitol., 2008;38:585-598.
[52] Liebler DC. Antioxidant reactions of carotenoids. Ann NY Acad Sci., 1996;691:20-31.
[53] Bendich AJ. Carotenoids and the immune response. J Nutr., 1989;119:112-115.
[54] Francheschi S, Bidoli E, La Vecchia C, Talamini R, D'Avanzo B, Negri E. Tomatoes and risk of digestive-tract cancer. Int J Cancer, 1994;50:181-184.
[55] Parker RS. Carotenoids in human blood and tissues. J Nutr., 1989;119:101-104.
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