International Journal of Chemical and Biomolecular Science
Articles Information
International Journal of Chemical and Biomolecular Science, Vol.1, No.4, Dec. 2015, Pub. Date: Sep. 11, 2015
Development and Validation of a RP-HPLC Method with PDA Detection for the Simultaneous Estimation of Acetylsalicylic Acid, Paracetamol and Caffeine in Fixed Dose Combination Tablets
Pages: 211-217 Views: 7346 Downloads: 2454
Authors
[01] Akwasi Acheampong, Department of Chemistry, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
[02] Sylvester Addai-Arhin, Department of Chemistry, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana; Department of Pharmaceutical Science, Faculty of Medicine and Health Science, Kumasi Polytechnic, Kumasi, Ghana.
[03] J. Apau, Department of Chemistry, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
[04] Wilfred O. Gyasi, Department of Chemistry, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Abstract
Acetylsalicylic acid and paracetamol are often formulated together in combination with caffeine, an adjuvant, for the relief of pain, fever and inflammations. A new RP-HPLC method with PDA detection has been developed and validated for the simultaneous estimation of acetylsalicylic acid, paracetamol and caffeine in fixed dose combination tablets. A Brownlee analytical column RP-C8 (5 µm, 150 x 4.6 mm) was used as the stationary phase and a mobile phase composed of acidic water/methanol mixture (60/40 v/v). Isocratic elution was employed. All the three components were eluted within 5.5 minutes with retention times of 2.05 ± 0.0062 for Paracetamol, 2.45 ± 0.0030 for Caffeine, and 5.03 ± 0.0140 for acetylsalicylic acid. The method was found to be accurate with mean recoveries of 99.39 ± 1.58 %, 99.69 ± 1.45 % and 100.56 ± 1.60 % for acetylsalicylic acid, paracetamol and caffeine respectively. It was also found to be linear (R2 ˃ 0.99), precise (RSD ˂ 2.0), specific, robust, sensitive and cost effective. Two brands of tablets containing the three active ingredients were successfully assayed by the validated method. The validated method can be used in routine quality control analysis of fixed dose combination tablets containing these three active ingredients.
Keywords
Acetylsalicylic Acid, Paracetamol, Caffeine, Validation, RP-HPLC, Multi-component Drug
References
[01] Abu-Qare, A. W., & Abou-Donia, M. B. (2001). A validated HPLC method for the determination of pyridostigmine bromide, acetaminophen, acetylsalicylic acid and caffeine in rat plasma and urine. Journal of pharmaceutical and biomedical analysis, 26(5), 939-947.
[02] Ahuja, S., Rasmussen, H. HPLC Method Development for Pharmaceuticals. Volume 8. Johnson and Johnson Pharmaceutical Research and Development, LLC. Raritan; New Jersey: 2007.p. 1–533.
[03] Bouhsain, Z., Garrigues, S., & de la Guardia, M. (1997). PLS-UV spectrophotometric method for the simultaneous determination of paracetamol, acetylsalicylic acid and caffeine in pharmaceutical formulations. Fresenius' journal of analytical chemistry, 357(7), 973-976.
[04] Chandra, P., Rathore, A. S., Lohidasan, S., & Mahadik, K. R. (2012). Application of HPLC for the simultaneous determination of aceclofenac, paracetamol and tramadol hydrochloride in pharmaceutical dosage form. Scientia pharmaceutica, 80(2), 337.
[05] Chandra, R., and Sharma, K.D. (2013). Quantitative determination of paracetamol and caffeine from formulated tablets by reversed phase-HPLC separation technique. International Journal of Chromatographic science, 3(2), 31-34.
[06] César Da Costa, I., Nogueira, F. H. A., & Pianetti, G. A. (2008). Simultaneous determination of artemether and lumefantrine in fixed dose combination tablets by HPLC with UV detection. Journal of Pharmaceutical and biomedical analysis, 48(3), 951-954.
[07] Franeta, J. T., Agbaba, D., Eric, S., Pavkov, S., Aleksic, M., & Vladimirov, S. (2002). HPLC assay of acetylsalicylic acid, paracetamol, caffeine and phenobarbital in tablets. Il Farmaco, 57(9), 709-713.
[08] Karim, M. M., Jeon, C. W., Lee, H. S., Alam, S. M., Lee, S. H., Choi, J. H., & Das, A. K. (2006). Simultaneous determination of acetylsalicylic acid and caffeine in pharmaceutical formulation by first derivative synchronous fluorimetric method. Journal of fluorescence, 16(5), 713-721.
[09] Kenkel, J. Analytical Chemistry for Technicians. Third Edition. Published by CRC Press LLC; USA: 2003. p. 1–545.
[10] La Guardia, M. (1996). Simultaneous stopped-flow determination of paracetamol, acetylsalicylic acid and caffeine in pharmaceutical formulations by Fourier transform infrared spectrometry with partial least-squares data treatment. Analyst, 121(12), 1935-1938.
[11] Li, S., Zhang B.O., and Zhang, N. (2010). Network target for screening synergistic drug combination with application to traditional Chinese medicine. BMC Systems Biology, 5(1), 1-13.
[12] Mackey, T. K., & Liang, B. A. (2011). The global counterfeit drug trade: patient safety and public health risks. Journal of pharmaceutical sciences, 100(11), 4571-4579.
[13] Malakar, P., Deb, A. R., Adhikary, S., Ahmed, S., & Maloth, R. (2013). Simultaneous estimation of phenylephrine hydrochloride, paracetamol, caffeine and cetirizine dihydrochloride from tablet dosage form using RP-HPLC. International Journal of Biological & Pharmaceutical Research, 4, 368-376.
[14] Murtaza, G., Khan, S. A., Shabbir, A., Mahmood, A., Asad, M. H. H. B., Farzana, K., & Hussain, I. (2011). Development of a UV-spectrophotometric method for the simultaneous determination of aspirin and paracetamol in tablets. Scientific research and Essays, 6(2), 417-421.
[15] Ramos-Martos, N., Aguirre-Gómez, F., Molina-Díaz, A., & Capitán-Vallvey, L. F. (2001). Application of liquid chromatography to the simultaneous determination of acetylsalicylic acid, caffeine, codeine, paracetamol, pyridoxine, and thiamine in pharmaceutical preparations. Journal of AOAC International, 84(3), 676-683.
[16] Sanghavi B, and Srivastava A. (2010). Simultaneous voltammetric determination of acetaminophen, aspirin and caffeine using an in situ surfactant-modified multiwalled carbon nanotube paste electrode. Electrochimica Acta, 55, 8638-8648.
[17] Sawant, R., Bhangale, L., Joshi, R., & Lanke, P. (2010). Validated spectrophotometric methods for simultaneous estimation of Paracetamol, Domperidone and Tramadol HCl in pure and tablet dosage form. Journal of chemical metrology, 4(1), 21-27.
[18] Sawyer, M., & Kumar, V. (2003). A rapid high-performance liquid chromatographic method for the simultaneous quantitation of aspirin, salicylic acid, and caffeine in effervescent tablets. Journal of chromatographic science, 41(8), 393-397.
[19] Sena, M. M., & Poppi, R. J. (2004). N-way PLS applied to simultaneous spectrophotometric determination of acetylsalicylic acid, paracetamol and caffeine. Journal of pharmaceutical and biomedical analysis, 34(1), 27-34.
[20] Singh, R. (2013). HPLC Method Development and Validation - An Overview. J Pharm Educ Res., 4(1), 26–33.
[21] Skoog, D. A., West, D. M., Holler, J. F., Crouch, S. R. Fundamentals of Analytical Chemistry. 8th Edition. Published by David Harris, Belmont; USA: 2004. p. 1–1157.
[22] Suresh, S. K., Latif, J. D., Krishnamurthy, B., Prashant, B., Vasantharaju, S. G., Udupa, N. (2010). Analytical Method Development and Validation for Aspirin. International Journal of ChemTech Research, 2(1), 389–399.
[23] Tsvetkova, B., Pencheva, I., Zlatkov, A., & Peikov, P. (2012). Simultaneous high-performance liquid chromatography determination of paracetamol and ascorbic acid in tablet dosage forms. African Journal of Pharmacy and Pharmacology, 6(17), 1332-1336.
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