International Journal of Chemical and Biomolecular Science
Articles Information
International Journal of Chemical and Biomolecular Science, Vol.1, No.4, Dec. 2015, Pub. Date: Nov. 12, 2015
Influence of IgG Separated from Blood Plasma of Patients with Ischemic Stroke on the Process Platelet's Proteins Secretion
Pages: 278-283 Views: 2418 Downloads: 1183
Authors
[01] Katrii T. B., Department of Biochemistry, Educational and Scientific Centre "Institute of Biology", Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.
[02] Vovk T. B., Department of Biochemistry, Educational and Scientific Centre "Institute of Biology", Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.
[03] Kravchenko N. K., Department of Biochemistry, Educational and Scientific Centre "Institute of Biology", Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.
[04] Savchuk O. M., Department of Biochemistry, Educational and Scientific Centre "Institute of Biology", Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.
[05] Ostapchenko L. I., Department of Biochemistry, Educational and Scientific Centre "Institute of Biology", Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.
Abstract
Influence of IgG accumulated in the blood plasma of patients with different types of ischemic stroke (atherothrombotic and cardioembolic) and with another neurological diseases (without stroke in anamneses) on the platelets was investigated. Influence of thrombin and collagen was tested too. Presence in the medium of incubation of most common proteins like Plasminogen activator inhibitor (PAI-1), von Willebrand factor (vWF), Heat shock proteins (Hsp-60, Hsp-70) were detected through the Western blot method.
Keywords
IgG, PAI-1, FvW, Hsp-60, HsP-70, Ischemic Stroke
References
[01] Murray CJL and Lopez AD. The global burden of disease.// Harvard school of public health. 1996.
[02] Ian M. Graham1, Marie-Therese Cooney1, Alexandra Dudina1, Sophie Squarta2. What is my risk of developing cardiovascular disease//European Journal of Cardiovascular Prevention and Rehabilitation. – 2009. – Vol. 16 (Suppl. 2). – P. 2–7.
[03] Mark Rubin, Bart Demaerschalk. The use of telemedicine in the management of acute stroke. // Neurosurg Focus. -2014. - 36(1).
[04] Hoyert DL and Xu J. Deaths: preliminary data for 2011. National vital statistics reports: from the Centers for Disease Control and Prevention, National Center for Health Statistics, National Vital Statistics System. -2012;61(6):1-52.
[05] The World Health Report: 2002: Reducing risks, promoting healthy life// World Health Organization. - 2002.
[06] Наказ МОЗ України від 3.08.2012 р. № 602 “Уніфікований клінічний протокол медичної допомоги. Ішемічний інсульт (екстрена, первинна, вторинна (спеціалізована) медична допомога, медична реабілітація)“. - 2012. – 120 с.
[07] Wolfe C. D. A. The impact of stroke // Brit.Med.Bull. - 2000. – Vol.56, № 2. – Р.275-286.
[08] Hirsh, Jack, et al. // Hemostasis and thrombosis: basic principles and clinical practice. - 2006.
[09] Martin, J, Shaw T., Heggie J., "The biological significance of platelet volume: its relationship to bleeding time, platelet thromboxane B 2 production and megakaryocyte nuclear DNA concentration // Thrombosis research. - 32.5. – 1983. – P. 443-460.
[10] Jenne, C, Urrutia R., Kubes P., Platelets: bridging hemostasis, inflammation, and immunity.// International journal of laboratory hematology. - 35.3. – 2013. – P. 254-261.
[11] Andrew S., Platelets: more than a sack of glue.// ASH Education Program Book. - 2014. - 1. - P.400-403.
[12] Вовк, Т. Б., Савчук O. M., Остапченко Л. І. Аутоімунні антитіла до ключових компонентів системи зсідання крові, які утворюються в кровотоці при системному червоному вовчаку//Фізика живого. 18.3.-2010.- C.10.
[13] Вовк Т. Б. Вплив Ig G хворих на системний червоний вовчак на показники хронометричних тестів. //Медична хімія.-2013.- 15, № 3. C.40-43.
[14] Titani K, Kumar S, Takio K, Ericsson LH, Wade RD, Ashida K, Walsh KA, Chopek MW, Sadler JE, Fujikawa K: Amino acid sequence of human von willebrand factor. Biochemistry 1986, 25(11):3171–3184.
[15] Pier Mannuccio, von Willebrand Factor A Marker of Endothelial Damage?// Arterioscler Thromb Vasc Biol. 1998 Sep; 18(9): 1359-1362.
[16] Stockschlaeder M, Schneppenheim R, Budde U. Update on von Willebrand factor multimers: focus on high-molecular-weight multimersand their role in hemostasis // Blood Coagul Fibrinolysis. 2014 Apr; 25(3):206-16.
[17] J. S. Giuliano Extracellular heat shock proteins: alarmins for the host immune system // Open Inflammation J., vol. 4 (1-M6), p. 49—60, 2011.
[18] Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose / Ey P. L., Prowse S. J., Jenkin C. R. // Immunochemistry. - 1978. - Vol. 15, №17. - P.429-436.
[19] Атеротромботический инсульт: клинические показатели и параметры тромбоцитарного гемостаза у пациентов в остром периоде / В.В. Голдобин, Е.Г. Клочева, П.М. Асадуллаева [та ін.] // Саратовский научно-медицинский журнал. – 2012. – Т. 8, № 4. – С. 954–957.
[20] Selected methods for antibody and nucleic acid probes / S. Hockfield, S. Carlson, C. Evans [та ін.]., 1993. – Vol. 1. – 679 p.
[21] Harlow E. D. Antibodies / E. D. Harlow, D. Lane. – New York: Cold Spring Harbor Laboratory Press, 1988. – 726 p.
600 ATLANTIC AVE, BOSTON,
MA 02210, USA
+001-6179630233
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - American Institute of Science except certain content provided by third parties.