International Journal of Chemical and Biomolecular Science
Articles Information
International Journal of Chemical and Biomolecular Science, Vol.5, No.1, Mar. 2019, Pub. Date: Mar. 5, 2019
Current Status and Opportunities of Plant Tissue Culture in Ethiopia
Pages: 19-28 Views: 127 Downloads: 655
[01] Temam Abrar, Department of Biotechnology, College of Natural Sciences, Arba Minch University, Arba Minch, Ethiopia.
[02] Haniza (Zhul-Hijja) Temam, Department of Biotechnology, College of Natural Sciences, Arba Minch University, Arba Minch, Ethiopia.
[03] Wesila Redwan, Department of Biotechnology, College of Natural Sciences, Arba Minch University, Arba Minch, Ethiopia.
Ethiopia is one of the populous nations of Africa where 80% of its inhabitants rely on agriculture as a source of income, which is also the basis for the national economy. But, long-standing traditional farming practices could not bring the desired agricultural productivity and thus hamper the development of national economy. In this regard, biotechnology particularly tissue culture is the likely opportunity for improving agricultural productivity in the Ethiopian agricultural system, thereby boosting the national economy. Indeed, in the past conventional selective breeding and cross-fertilization of plants and animals played a vital role in agricultural productivity. However, these approaches have limitations in handling contemporary and emerging challenges unless they are supported by modern biotechnological tools. Yet, many developing countries including Ethiopia could not get benefits from modern biotechnological tools and products. To resolve this, the Ethiopian Government recently set national biotechnology road map, consequently designing and implementing policies and strategies related to modern biotechnology, prioritizing the agricultural sectors. To this effect, national universities, research institutes and some private enterprises, are playing a promising role in producing skilled manpower and started conducting research in biotechnology. Nationally, Ethiopian Agricultural Research Institute (EARI), Tigray Biotechnology Center (TBC) and Addis Ababa University (AAU) take the leading role particularly in doing researches related to plant tissue culture and at the same time disseminating the research output to the stakeholders especially unthinkable quantity of diseases-free plantlets. Nowadays, Ethiopian government have creating public awareness and engagement as well as in building public confidence, trust and acceptance of plant tissue culture in the country. Currently sugarcane production in Ethiopia has given due attention and under great expansion work, this is also allied with an environment-friendly plant tissue culture for large scale production of sugarcane plantlets to feed the sugar industries. This is promising opportunities for Ethiopia’s Sugar industries.
Ethiopia, Plant Tissue Culture, Biotechnology, In vitro, Agriculture, Explants
[01] CSA (Central Statistical Agency) (2014). Press Conference on Population Projection for Ethiopia, Addis Ababa, Ethiopia.
[02] Mohammed AS (2013). Plant tissue culture biotechnology in Ethiopia: Challenges and opportunities, Research & Reviews in BioSciences, 7 (7): (249-255).
[03] Belete G (2017). Review on In Vitro Propagation of Sugarcane to Advance the Value of Tissue culture. Agricultural Research & Technology, 5 (4): 555670. DOI: 10.19080/ARTOAJ.2017.05.555670.
[04] Chekol C and Gebreyohannes M (2018). Application and Current Trends of Biotechnology: a Brief Review. Austin Journal of Biotechnology and Bioengineering. 5 (1): 1088.
[05] Tolera B and Shimelis D (2016). Comparison of Micropropagated and Conventional Raised Sugarcane Planting Materials as Initial Source of Seed Cane at Metahara Sugar Estate, Ethiopia. Advances in Tissue Engineering and Regenerative Medicine, 1 (2): 1-3.
[06] FDRE (Federal Democratic Republic of Ethiopia) (2014). National Biotechnology Road Map. (Amharic Version).
[07] Hussain A, Qarshi IA, Nazir H, Ullah I (2012). Plant tissue culture: Current status and opportunities, Recent Advances in Plant in vitro Culture; Licensee In Tech, Open science/open mind, http: / /, accessed at January.
[08] Sime M (2013). The Effect of Different Cane Portions on Sprouting, Growth and Yield of Sugarcane (Saccharum spp. L.). International Journal of Scientific and Research Publications, 3 (1): 1-3.
[09] ESC (Ethiopian Sugar Corporation) (2015). Sweet Newsletter 4: 1.
[10] EIAR (Ethiopian Institute of Agricultural Research) (2016). Agricultural Biotechnology Research Activities and Achievement. Holetta, Ethiopia.
[11] Tena E, Mekbib F, Shimelis H and Mwadzingeni L (2016). Sugarcane production under smallholder farming systems: Farmers preferred traits, constraints and genetic resources. Cogent Food and Agriculture, 2: 1-15.
[12] Kassaye E, Dagnaw BB (2015). In vitro optimization of the protocol for micropropagation of Plum (Prunus Salicina L. Var. Methley) from nodal explants. Biotechnology International, 8 (4): 137-148.
[13] Gemechu FK, Feyissa T (2016). Hyperhydricity (Vitrification) of micropropagated shoots of two Grapevine (Vitis vinifera L.) Cultivars. International Journal of Current Trends in Pharmacobiology and Medical Sciences, 1 (3): 71-77.
[14] Ayenew B, Tefera W, Kassahun B (2012). In vitro propagation of Ethiopian ginger (Zingiber officinale) cultivars: Evaluation of explant types and hormone combinations. African Journal of Biotechnology, 11 (16): 3911-3918.
[15] Gebeyehu A (2013). Effect of different combinations of 6-benzyl amino purine and naphthalene acetic acid on multiple shoot proliferation of plantain (Musa spp.) cv. Matoke from meristem derived explants. African Journal of Biotechnology, 12 (7): 709-719.
[16] Disasa T, Feyissa T, Dagne K (2011). In vitro regeneration of Niger (Guizotia abyssinica L.). International Journal of Biosciences, 1 (6): 110-118.
[17] Setargie A, Mekbib F, Abraham E (2015). In vitro propagation of Papaya (Carica papaya L.). World Journal of Agricultural Sciences, 11 (2): 84-88.
[18] Abu G, Mekbib F and Teklewold A (2014). Effect Of Genotype On In vitro Propagation Of Elite Sugarcane (Saccharum Officinarum L.) Varieties Of Ethiopian Sugar Estates. International Journal of Technology Enhancements and Emerging Engineering Research, 2 (6): 123-128.
[19] Tolera B, Diro M, Belew D (2014). In vitro aseptic culture establishment of Sugarcane (Saccharum officinarum L.) varieties using shoot tip explants. Advances in Crop Science and Technology, 2 (3): 1-6.
[20] Gezahegn G, Mekbib F (2016). In vitro regeneration of disease free enset [Ensete ventricosum (Welw) Cheesman] planting materials from bacterial wilt diseased plants using shoot tip culture. African Journal of Biotechnology, 15 (40): 2192-2201.
[21] Getahun T, Feyissa T, Gugsa L (2013). Regeneration of plantlets from unpollinated ovary cultures of Ethiopian Wheat (Triticum turgidum and Triticum aestivum). African Journal of Biotechnology, 12 (39): 5754-5760.
[22] Demeke Y, Tefera W, Dechassa N, Abebie B (2014). Effect of plant growth regulator on in vitro cultured nodal explant of cassava (Manihot esculenta Crantz) clones. African Journal of Biotechnology, 13 (28): 2830-2839.
[23] Aga E, Khillare Y (2017). In vitro regeneration of shoots from apical and auxiliary bud explants of Coffea arabica L. Journal of Medicinal Plants Studies, 5 (1): 09-12.
[24] Mengesha A, Ayenew B, Tadesse T (2013). Acclimatization of in vitro propagated Pineapple (Ananas comosuss L.) plantlets to ex vitro condition in Ethiopia. American Journal of Plant Sciences, 4 (2): 317-323.
[25] Terefe G, Sunil TH, Tolera B (2017). Ex-Vitro Propagation of Micropropagated Sugarcane (Sacharum Officinarum L.) Genotypes Using Plant Growth Regulators IBA and BAP. Journal of Applied Biotechnology & Bioengineering, 2 (3): 1-7.
[26] Yifter M, Shbatu DB, Mekbib F, Abraha E (2013). In vitro regeneration of four Ethiopian varieties of Sesame (Sesamum indicum L.) using anther culture. Asian Journal of Plant Science, 12 (5): 214-218.
[27] Derso C, Feyissa T (2015). Micropropagation of Yeheb from shoot tip: An endangered multipurpose shrub. Plant Science International, 2: 01-12.
[28] Ibrahim M, Tolera B, Aman J and Negi T (2016). Evaluation of Tissue Culture Raised Sugarcane Planting Materials against their Donor Conventional Seed Sources as Initial Source of Seed Cane at Tendaho Sugar Development Project, North-Eastern Ethiopia. Journal of Horticulture, 3 (1): 1-4.
[29] European Commission (2002). Life Science and Biotechnology- A Strategy for Europe, Luxembourg, Office for Official Publications of the European Communities.
[30] FDRE (Federal Democratic Republic of Ethiopia) (2013). Industrial Development Strategy. (Amharic Version).
[31] FDRE (Federal Democratic Republic of Ethiopia) (2015). Ethiopia’s Climate-Resilient Green Economy: Green Economy Strategy, Addis Ababa, Ethiopia.
[32] Mekonnen T, Diro M, Sharma M and Negi T (2014). Protocol optimization for in vitro mass propagation of two sugarcane (Saccharum officinarum L.) clones grown in Ethiopia. African Journal of Biotechnology, 13 (12): 1358-1368.
[33] Abebayehu M, Abebe M and Frehiwot W (2014). Developing an Enabling Scientific Equipment Policy in Africa: Ethiopia Country Study, Addis Ababa Ethiopia:
[34] Masiga CW, Mneney E, Wachira F, and Mugoya C (2013). Situational Analysis of the Current State of Tissue Culture Application in the Eastern and Central Africa Region. 38p. Association for Strengthening Agricultural Research In East and Central Africa (ASARECA):
[35] United Nations: Economic Commission for Africa (2015). Review report on progress in the implementation of sustainable development commitments related to biotechnology in Africa. Addis Ababa, Ethiopia:
[36] Ahmed W, Feyissa T, Disasa T (2013). Somatic embryogenesis of a Coffee (Coffea arabica L.) hybrid using leaf explants. Journal of Horticultural Science and Biotechnology, 88 (4): 469-475.
[37] Seyoum Y, Mekbib F (2014). In vitro germination and direct shoot induction of Yeheb (Cordeauxia edulis). Agriculture, Forestry and Fisheries, 3 (6): 452-458. doi: 10.11648/j.aff.20140306.13.
[38] Dagnew A, Shibru S, Debebe A, Lemma A, Dessalegn L, Berhanu B, Beyene W, Miguelez YS (2012). Micropropagation of Banana varieties (Musa spp.) using shoot-tip culture. Ethiopian Journal of Agricultural Sciences, 22: 14-25.
[39] Manahlie B, Feyissa T (2014). Micropropagation of Kebericho: An endangered Ethiopian medicinal plant. American Journal of Plant Sciences 5: 3836-3847.
[40] Hailu T, Abera B, Daksa J (2015). In vitro direct organogenesis protocol for mass propagation of an elite Ethiopian hot Pepper (Capsicum annuum L.) Cultivar: Mareko Fana. American Journal of Plant Sciences, 6: 1435-1443.
[41] Bekele F, Abera B, Getahun M (2013). In vitro propagation of Anchote (Coccinia abyssinica) (Lam.) Cogn. African Journal of Plant Science, 7 (6): 253-264.
[42] Tefera W, Wannakrairoj S (2006). Synergistic effects of some plant growth regulators on in vitro shoot proliferation of Korarima (Aframomum corrorima (Braun) Jansen). African Journal of Biotechnology, 5 (10): 1894-1901.
[43] Fufa M, Diro M (2014). Microtuber induction of two Potato (Solanum tuberosum L.) varieties. Advances in Crop Science and Technology, 2: 122. doi: 10.4172/2329-8863.1000122.
[44] Alula K (2014). In vitro mass propagation of sweet potato [Ipomoea batatas (L.) Lam]. MSc. Thesis, Addis Ababa University.
[45] Kahsay B M (2015). In vitro propagation of Ethiopian Lowland Bamboo [Oxytenanthera abyssinica (A. Rich. Munro)] from seed culture. MSc. Thesis, Addis Ababa University.
[46] Tafere AG, Mekbib F, Admassu B (2014). In vitro propagation of Ethiopian mustard (Brassica carinata A. BRAUN). African Journal of Biotechnology, 13 (48): 4438-4448.
[47] Gugsa L, Kumlehn J (2011). Somatic embryogenesis and massive shoot regeneration from immature embryo explants of tef. Biotechnology Research International, 1: 1-7.
[48] Sawant R A and Meti N T (2016). Cost Effective Techniques for In vitro Propagation of Two Varieties of Sugarcane. International Journal of Current Research in Biosciences and Plant Biology, 3 (5): 65-72.
[49] Temam AH and Alemayehu LA (2017). Sugarcane (Saccharum Officinarum L) Tissue Culture In Ethiopia: Opportunities For Ethiopia’s Sugar Industries. International Journal Of Scientific & Technology Research 6 (08): 398-406
[50] Tesfa M, Admassu B and Bantte K (2016). In vitro Shoot Multiplication of Elite Sugarcane (Saccharum officinarum L.) Genotypes Using Liquid Shake Culture System. Journal of Biology, Agriculture and Healthcare 6 (1): 35-40.
MA 02210, USA
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - American Institute of Science except certain content provided by third parties.