International Journal of Life Science and Engineering
Articles Information
International Journal of Life Science and Engineering, Vol.2, No.1, Jan. 2016, Pub. Date: Feb. 1, 2016
In Vitro Direct Shoot Regeneration of Plantlet in Medicinal Plant of Oenothera biennes
Pages: 1-7 Views: 908 Downloads: 677
Authors
[01] Narjes Sattar, Department of Agricultural Science, Payame Noor University, Tehran, Iran.
[02] Elham Azizi, Department of Agricultural Science, Payame Noor University, Tehran, Iran.
[03] Mahmood Valizadeh, Department of Agricultural Science, Payame Noor University, Tehran, Iran.
Abstract
Evening primrose (Oenotherabiennis L.) is a two-year herbaceous plant and one of the important species of medicinal plants in the world. Oil in the plant contains essential fatty acids that the body cannot produce it. In order to investigate explants callusing and regeneration of evening primrose, two separate experiments were conducted as factorial based on a completely randomized design with three replications in tissue culture laboratory of Payame Noor University of Mashhad during 2015. In the first experiment, treatments included naphthalene acetic acid (NAA) at four levels (0, 0.5, 1, 2 mg/L), 6-Benzylaminopurin (BAP) at five levels (0, 0.25, 0.5, 1, 2 mg/L) and three explants (hypocotyls, embryo and leaf). In the second experiment, treatments included BAP at four levels (0, 0.5, 1, 2 mg/L), 2.4-Dichlorophenoxyacetic acid (2.4.D) at four levels (0, 0.5, 1, 2 mg/L) and three explants (hypocotyls, embryo and leaf). In the first experiment, the highest number of callus and regeneration explants was recorded at 0 mg/L and 2 mg/L of BAP respectively. As the concentration of BAP was increased from 0 to 1 mg/L, regeneration increased. Also with increasing concentrations of NAA, callus number was increased to 1 mg/L and continued to decrease in 2 mg /L. The most regeneration in embryo’s explant was obtained at 2 mg/L of BAP and 1mg/L of NAA concentration. The most direct regeneration was observed in leaf explants at 0.25 mg/L of BAP concentration. In the second experiment with increasing of BAP level, relative frequency of callus decreased to 1 mg/L continued increasing to 2 mg/L, while regeneration frequency was increased.Callus induction was seen in all explants with different concentration of BAP and 2, 4-D. The most callus induction was seen in leaf and hypocotyl explants, at media containing 0.5 mg/L BAP in combination with 0.5 mg/L of 2, 4-D (3.33).
Keywords
Evening Primrose, Medicinal Plant, Tissue Culture, Somatic Embryogenesis
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