International Journal of Preventive Medicine Research
Articles Information
International Journal of Preventive Medicine Research, Vol.1, No.4, Oct. 2015, Pub. Date: Jul. 16, 2015
Evaluation and Comparative in vitro and in vivo Analysis of Anti-inflammatory Potential of Ocimum sanctum Extracts from Tropical and Alpine Regions of Nepal
Pages: 201-206 Views: 4328 Downloads: 1187
[01] Subodh Chataut, Department of Pharmacy, Crimson College of Technology, Pokhara University, Rupandehi, Nepal.
[02] Sabina Sharma, Department of Pharmacy, Crimson College of Technology, Pokhara University, Rupandehi, Nepal.
[03] Shiva Kumar Sah, Department of Pharmacy, Crimson College of Technology, Pokhara University, Rupandehi, Nepal.
[04] Pratima Shrestha, Department of Pharmacy, Crimson College of Technology, Pokhara University, Rupandehi, Nepal.
[05] Sarmila Nepali, Department of Immunology, Chonbuk National University Medical School, Jeonju, Korea.
[06] Shyam Kumar Mallik, College of Pharmacy, Institute of Pharmaceutical Research and Development, Wonkwang University, Iksan, Jeonbuk, Korea.
[07] Barun Poudel, Department of Immunology, Chonbuk National University Medical School, Jeonju, Korea.
[08] Ram Prasad Bhusal, Department of Pharmacy, Crimson College of Technology, Pokhara University, Rupandehi, Nepal; Department of Organic and Medicinal Chemistry, School of Chemical Sciences, University of Auckland, Auckland, New Zealand.
The purpose of this study was to evaluate and compare the anti-inflammatory efficacy of Ocimum sanctum (OS) (Tulsi) extracts obtained from two different regions; Butwal (tropical) and Gorkha (alpine) of Nepal. The anti-inflammatory activities of the extracts were determined in vitro using heat-induced albumin denaturation assay and in vivo by using formalin-induced rat paw edema model. In both of these experiments, commercial anti-inflammatory drug, indomethacin, was utilized to justify the results. The extracts from both the regions were effective in inhibition of heat-induced albumin denaturation (HIAD) and attenuation of formalin-induced rat paw edema development. Notably, the extract from alpine Gorkha region was more potent in reduction of HIAD and edema development in rats injected with formalin in their paws, suggesting that OS from alpine regions could have greater contents of constituents mediating the anti-inflammatory actions of OS. The present data may provide the scientific basis for the traditional use of OS and suggests that OS from alpine regions could be studied for design and development of anti-inflammatory therapeutic agents. Further research is required to address the mechanisms and difference in contents of OS between the two regions of Nepal to elicit anti-inflammatory actions.
Ocimum Sanctum, Tulsi, Anti-Inflammation, Alpine, Tropical, Nepal
[01] Okin, D. and Medzhitov, R. (2012). Evolution of inflammatory diseases. Curr. Biol., 22(17): R733-740.
[02] Shen, H., Kreisel, D. and Goldstein, D.R. (2013). Processes of sterile inflammation. J. Immunol., 191(6): 2857-2863.
[03] Yang, E.J., Moon, J.Y., Kim, S.S., Yang, K.W., Lee, W.J. and Lee, N.H. (2014). Jeju seaweeds suppress lipopolysaccharide-stimulated proinflammatory response in RAW 264.7 murine macrophages. Asian. Pac. J. Trop. Biomed., 4(7): 529-537.
[04] Mohammed, M.S., Alajmi, M.F., Alam, P., Khalid, H.S., Mahmoud, A.M. and Ahmed, W.J. (2014). Chromatographic finger print analysis of anti-inflammatory active extract fractions of aerial parts of Tribulus terrestris by HPTLC technique. Asian. Pac. J. Trop. Biomed., 4(3): 203-208.
[05] Suke, S.G., Negi, H., Mediratta, P.K., Banerjee, B.D. and Sharma, K.K. (2013). Anti-arthritic and anti-inflammatory activity of combined pioglitazone and prednisolone on adjuvant-induced arthritis. Eur. J. Pharmacol., 718: (1-3): 57–62.
[06] Ali, K., Ashraf, A. and Nath Biswas, N. (2012) Analgesic, anti-inflammatory and anti-diarrheal activities of ethanolic leaf extract of Typhonium trilobatum L. Schott. Asian. Pac. J. Trop. Biomed., 2(9): 722–726.
[07] Wildi, B. and Lutz, C. (1996). Antioxidant composition of selected high alpine plant species from different altitudes. Plant. Cell. Environ., 19(2): 138-146.
[08] Cohen, M.M. (2014). Tulsi-Ocimum sanctum: A herb for all reasons. J. Ayurveda. Integr. Med., 5(4): 251-259.
[09] Katla, V.R., Syed, R., Kuruva, C.S., Kuntrapakam, H.K. and Chamarthi, N.R. (2013). Synthesis of novel phosphorylated guanidine derivatives from cyanamide and their anti-inflammatory activity. Chem. Pharm. Bull. (Tokyo). 61(1): 25-32.
[10] Alajmi, M.F. and Alam, P. (2014). Anti-inflammatory activity and qualitative analysis of different extracts of Maytenus obscura (A. Rich.) Cuf. By high performance thin layer chromatography method. Asian. Pac. J. Trop. Biomed., 4(2): 152-157.
[11] Choudhury, S.S., Bashyam, L., Manthapuram, N., Bitla, P., Kollipara, P. and Tetali, S.D. (2014). Ocimum sanctum leaf extracts attenuate human monocytic (THP-1) cell activation. J. Ethnopharmacol., 154(1): 148-155.
[12] Wang, G.W., Huang, B.K. and Qin, L.P. (2012). The genus Broussonetia: a review of its phytochemistry and pharmacology. Phytother. Res., 26(1):1-10.
[13] Chukwurah, P.N., Brisibe, E.A., Osuagwu, A.N. and Okoko, T. (2014). Protective capacity of Artemisia annua as a potent antioxidant remedy against free radical damage. Asian. Pac. J. Trop. Biomed., 4(Suppl 1): S92-S98.
[14] Chandra, S., Chatterjee, P., Dey, P. and Bhattacharya, S. (2012). Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian. Pac. J. Trop. Biomed., S178-S180.
[15] Jagtap, V.A., Agasimundim, Y.S., Jayachandran, E., and Sathe, B.S. (2011). In vitro anti-inflammatory activity of 2-amino-3-(substituted benzylidinecarbohydrazide)-4,5,6,7 tetrahydrobenzothiophenes. J. Pharm. Res., 4: 378-379.
[16] Shojaii, A., Motaghinejad, M., Norouzi, S. and Motevalian, M. (2015). Evaluation of anti-inflammatory and analgesic activity of the extract and fractions of Astragalus hamosus in animal models. Iran. J. Pharm. Res., 14(1): 263-269.
[17] Saini, N.K. and Singhal, M. (2012). Anti-inflammatory, analgesic and antipyretic activity of methanolic Tecomaria capensis leaves extract. Asian. Pac. J. Trop. Biomed., 2(11): 870-874.
[18] Ruth, A.F., Olaide, A.O. and Oluwatoyin, S.M. (2014). The aqueous root extract of Aristolochia ringens (Vahl.) Aristolochiaceae inhibits chemically-induced inflammation in rodents. Pak. J. Pharm. Sci., 27(6): 1885-1889.
[19] Shah, A.S. and Alagawadi, K.R. (2011). Anti-inflammatory, analgesic and antipyretic properties of Thespesia populnea Soland ex. Correa seed extracts and its fractions in animal models. J. Ethnopharmacol., 137(3):1504-1509.
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