International Journal of Preventive Medicine Research
Articles Information
International Journal of Preventive Medicine Research, Vol.1, No.5, Dec. 2015, Pub. Date: Dec. 30, 2015
The Effect of Route of Administration and Dose on the Immunogenicity and Protective Efficacy of Newcastle Disease Thermostable Vaccine I2 Strain
Pages: 276-281 Views: 2473 Downloads: 1697
Authors
[01] Wegdan H., Veterinary Research Institute, Soba, Khartoum, Sudan.
[02] Mahasin E., Veterinary Research Institute, Soba, Khartoum, Sudan.
[03] Khalafalla A. I., Faculty of Veterinary Medicine, University of Khartoum, Khartoum North, Sudan.
Abstract
Newcastle disease (ND) is one of the most important diseases in poultry worldwide. It is highly contagious affecting chickens other avian species and wild birds causing higher economic losses. vaccination is the best control strategy for the disease and many commercial live and killed vaccines being commonly used in poultry farms. In the present study, the effect of route of administration and dose on the immunogenicity and protective efficacy of the thermostable Newcastle disease vaccine (I2) was determined under Sudan field conditions. Three experiments were conducted to determine, firstly, the effect of routs of adminstration (namely intraocular (I/O), intranasal (I/N) and drinking water (D/W) and secondly, the dose of the vaccine (recommended dose and half, two and four times of the recommended dose) on the immunity of two weeks old commercial layer chicks under lab condition. In the third experiment, the immunogenicity of the vaccines I2 “I/O, I/N and D/W” and Komarov “I/N and D/W” in three weeks old commercial layers and indigenous village chickens was determined under field condition. The results of the three experimental trials indicated that, protection against the challenge infection was attained when chicks received two doses of the I2 vaccine via an intraocular route. The I/O route was found to be superior to the I/N which was confirmed to be better than the D/W route; as observed from the mean haemagglutination inhibition antibody (HI-Ab) titres and percentage of protection produced by the vaccine through these routes. When comparing the dose of the I2 vaccine, it was proved that even half of the recommended dose resulted in good immune response and protection. Moreover, doubling or even four times of the recommended dose gave 100% protection without any adverse post vaccination reactions. Results of the field trials showed better Ab response of I2 vaccine when given via an I/O route in three weeks old commercial layers and indigenous village chickens compared to K- vaccine of ND. Generally, at least HI antibody titre of 5.5 log2 is required for 100% protection against challenge infection.
Keywords
NDV, Thermostable Vaccine, Route, Dose, Safety, HI Test, Domestic Fowl, Sudan
References
[01] Adwar, T and Lukešová, D. (2008). Evaluation of thermostable vaccines against Newcastle disease in village chicken used in tropics and subtropics. Agricultura Tropica ET Subtropica: 41(2) 74-79.
[02] Alder, R.G. and Fringe, R. (1998). The use of thermostable Newcastle disease vaccine in Mozambique. In: Improving Family Poultry Production in Africa. Background information FAO.
[03] Alexander, D. J. (1991). Newcastle disease and other paramyxovirus infection. Diseases of Poultry. pp. 496-519. 9th edition. Iowa State University Press, Ames, Iowa, USA.
[04] Alexander, D. J. (2009).Ecology and epidemiology of Newcastle disease. In. Avian influenza and Newcastle disease. A field and laboratory manual 1st edn.. Edited by Capua, I and Alexander, D.J. Springer- Verlag Italia. Chapter 2 Pp 19-26.
[05] Alexander, D.J., Parsons, G. and Marshal, R. (1984). Infection of fowls with Newcastle disease virus by food contaminated with pigeon feces. Veterinary Record 115: 601-602.
[06] Ali, B.E. (1978). Response of chicks to vaccination with K strain of NDV under field conditions. Indian Veterinary Journal 55: 508-512.
[07] Allan, W. H. (1971). The problem of Newcastle disease. Nature (London) 234: 129-131.
[08] Allan, W. H. (1972). Newcastle disease control. Agriculture 79: 413-420.
[09] Allan, W. H., Lancaster, J.E. and Toth, B. (1978). Newcastle disease vaccines: their production and use. FAO Animal Production, Rome, Series No. 10 FAO.
[10] Anita, V. K. (1999). Results from Newcastle disease vaccine trials in Zimbabwe. Improving Family Poultry Production in Africa. Background information. FAO presented in Rabat Morocco, Feb 1999.
[11] Bensink, Z., Spradbrow, P.B. (1999). Newcastle disease virus strain I2--a prospective thermostable vaccine for use in developing countries. Veterinary Microbiology 16: 68 (1-2): 131-9.
[12] Bornstien, S., Arazi, A. R. and Samberg, Y. (1952). Some aspects of congenital passive immunity to Newcastle disease in chicks. The transfer of haemagglutination inhibitors from the maternal yolk to the chick. American Veterinary Research Journal 13: 373-378.
[13] Chu, H.P. (1960). A laboratory hand book on diagnosis of poultry disease. Rome, FAO. (Animal Health monogragp No. 2).
[14] Dawson, P.S. and Allan, W.H. (1973). The control of Newcastle disease by vaccinatio. In: 4th European Poultry Conference. British Poultry Science Ltd. Edinburgh. 591-598, Central Veterinary Laboratory, New Haw, Weybridge, Surrey, England.
[15] Foster, A., Chitukuro, H.R. and Tuppa, E. (1997). A field trial with thermostable V4 Newcastle disease vaccine in village in central Tanzania. Proceedings in FPD Workshop, M. Bour, Sengal Dec. 9-13. Issues in family poultry Research and Development.
[16] Gaffar Elamin, M.A., Khalafalla, A.I. and Ahmed, S.M. (1993). Observation on the use of Komarov strain of Newcastle disease vaccine in the Sudan. Tropical Animal Health and Production 25: 151-154.
[17] Kheir, S.A.M. (1992). Immune response to Newcastle disease vaccine (Komarov strain) given in drinking water. The Sudan Journal of Veterinary Science and Animal Husbandry 31: 14-19.
[18] Komarov, A. and Goldsmith, L. (1947). The use of live viruses in Palestine for the vaccination of poultry against Newcastle disease. Cornell Veterinarian 371: 368-372.
[19] Mahmood, M. Shahid; Siddique, F., Hussain, I., Ahmad, S.I. and Rafique, A. (2014): Thermostable vaccines for Newcastle disease: a review. World's Poultry Science Journal, Vol. 70, December 2014
[20] Meulemans, G. (1988). Control by vaccination. In: Alexander, D.J. (ed). Newcastle disease, pp. 318-332. Kluwer Academic Publisher, Boston, MA, USA.
[21] Murphy, F.A., Gibbs, E.P., Horzinek, M.C., and Suddert, M.J. (1999). Paramyxovirdae: Veterinary Virology, 412-428, third edition Academic Press USA.
[22] OIE (2012). Newcastle disease.. In Manual of diagnostic tests and vaccine for terrestrial animals .chapter 2-3-14 version updated in May 2012.
[23] Rajeswar, J.J. and Masilamony, P.R. (1993). Spray vaccine against ND. Indian Veterinary Journal 70: 404.
[24] Reed, L.J. and Muench. H. (1938). A simple method of estimating fifty percent end point. American Journal of Hygiene, 27: 493-497.
[25] Shuaib, M.A., Spalatin, J., McMillan, B. and Hanson, R.P. (1985). Studies on the development of a pelleted Newcastle disease virus (NDV) vaccine. Vaccine 3: 385-388.
[26] Spradbrow, P.B. (1992). Newcastle disease in village chickens. Control with thermostable oral vaccine. Proceedings of an international workshop held in Kuala Lumpur, Malaysia, 6-10 October, 1991. Pp. 189, ACIAR Proceedings No. 39.
[27] Wegdan, H.A., Khair, S.A.M., Bontsi, M. and Celia Abolink. (2010). Newcastle disease outbreaks in the Sudan from 2003 to 2006 were caused by viruses of genotype 5d. Virus genes 40: 106-110.
[28] Zakia, A.M., Ali, B.H., Mahasin, E. and Amal, M.M. (1983). The serological response of chicks to intranasal ND vaccination and re-vaccination. Sudan Journal of Veterinary Research 4: 99-103.
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