International Journal of Preventive Medicine Research
Articles Information
International Journal of Preventive Medicine Research, Vol.2, No.1, Feb. 2016, Pub. Date: Apr. 14, 2016
Aluminium Induced the Oxidative Stress Modification and a Behavioural Variation in Rat
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[01] Kharoubi O., Faculty of Natural and Life Science, Department of Biology, Laboratory of BTE-BD-PR, University of Oran 1 Ahmed Benbella, Oran, Algeria.
[02] Benyamina A., Faculty of Natural and Life Science, Department of Biology, Laboratory of BTE-BD-PR, University of Oran 1 Ahmed Benbella, Oran, Algeria.
[03] Hallal N., Faculty of Natural and Life Science, Department of Biology, Laboratory of BTE-BD-PR, University of Oran 1 Ahmed Benbella, Oran, Algeria.
[04] Benyattou I., Faculty of Natural and Life Science, Department of Biology, Laboratory of BTE-BD-PR, University of Oran 1 Ahmed Benbella, Oran, Algeria.
[05] Aoues A., Faculty of Natural and Life Science, Department of Biology, Laboratory of BTE-BD-PR, University of Oran 1 Ahmed Benbella, Oran, Algeria.
[06] Slimani M., Departments of Biology, University of Saida, Saida, Algeria.
Background: Aluminium (Al) poisoning is a potential factor in brain damage at even low and high levels, neurochemical dysfunction and severe behavioral troubles. Considering this effect, our study was carried out to investigate the effects of wormwood extract to restore enzymes activities, lipid peroxidation, carbonyl and behavioral changes induced by Al. Methods: twenty four Wistar rats were divided into four groups: one group was exposed to a mixture containing100 mg/kg body weight of AlCl3 in the drinking with wormwood extract (Al+Pl), another group received wormwood extract (200 mg/kg body weight), other group received water after stopped intoxication for 5 weeks (Al(-)) and a groups as control. Activities of lactate deshydrogenase (LDH), Catalase (Cat), thiobarbituric acid-reactive substances (TBARS) and carbonyl level were determined in the whole brain of rats and the grooming and locomotors activity were defined in all groups. Results: The behavioral test (locomotors, sniffing and grooming test) indicates a significant hyperactivity in the Al+Pl group compared with the control group. After treatment with wormwood extract, the Pl indicates a lower activity compared with control and Al+Pl group.The intoxicated group (Al+Pl) has a significantly increased TBARS and carbonyl value compared to control (P < 0.05) and, after treatment with the wormwood extract, a significant reduction was noted. The LDH activity decreased significantly (P < 0.05) in the Al+Pl group compared with the control, by –39% and was increased in catalase activity by +196%. After wormwood extract administration, LDH and Cat activity were significantly changed compared to Al+Pl group. Conclusion: These data suggest that aqueous wormwood extract may play a very useful role in reduction of the neurotoxicological damage induced by Aluminium. Administration of wormwood extract may induce a neurochemical change and attenuate the alteration in neurobehavioral activity, observed in the development of age related disorders, such as Alzheimer’s disease.
Aluminium, Behavioral Test, Brain, Catalase, Carbonyl, Lactate Deshydrogenase, Lipid Peroxidation
[01] Yang JL, Li YY, Zhang YJ, et al. Cell wall polysaccharides are specifically involved in the exclusion of aluminum from the rice root apex. Plant Physiology. 2008; 146: 602–611.
[02] Yumoto S, Nagai H, Matsuzaki H, Matsumura H, Tada W, Nagatsuma E,. Aluminum incorporation into the brain of rats fetuses and sucklings. Brain Research Bulleton; 2001, 55, 229-234.
[03] Perez-Gracia E., Blanco R., Carmona M., Carro E., Ferrer I. (2009). Oxidative stress damage and oxidative stress responses in the choroid plexus in Alzheimer’s disease. Acta Neuropathol. 118, 497
[04] Bassett CN, Montine TJ: Lipoproteins and lipid peroxidation in Alzheimer's disease. The Journal of nutrition, health & aging 2003, 7:24-29.
[05] Cossec J-C, Marquer C, Panchal M, Lazar AN, Duyckaerts C, Potier M-C: Cholesterol changes in Alzheimer's disease: methods of analysis and impact on the formation of enlarged endosomes. Biochimica et biophysica acta 2010, 1801: 839-45.
[06] Pan R, Qiu S, Lu D-xiang, Dong J: Curcumin improves learning and memory ability and its neuroprotective mechanism in mice. Chinese medical journal 2008, 121: 832-9.
[07] Kakkar V, Kaur IP: Evaluating potential of curcumin loaded solid lipid nanoparticles in aluminium induced behavioural, biochemical and histopathological alterations in mice brain. Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 2011, 49: 2906-13.
[08] Walton, J. R. (2009a). Brain lesions comprised of aluminum-rich cells that lack microtubules may be associated with the cognitive deficit of Alzheimer’s disease. Neuro Toxicology, 30(6), 1059-1069.
[09] Abu-Taweel GM, Ajarem JS, Ahmad M, 2012. Neurobehavioral toxic effects of perinatal oral exposure to aluminum on the developmental motor reflexes, learning, memory and brain neurotransmitters of mice offspring. Pharmacology Biochemistry and Behavior, 101(1): 49–56.
[10] Čanadanović-Brunet JM, Ðilas SM, Ćetković G, Tumbas VT. Free-radical scavenging activity of wormwood (Artemisia absinthium L.) extracts. J Sci Food Agric 2005; 85: 265-72.
[11] Osawa T. Protective role of dietary polyphenols in oxidative stress. Mech Ageing Dev 1999; 111: 133-9.
[12] Wiseman H, Okeily JD, Adlercreutz H, Mallet AJ, Bowery EA, Sanders AB. Isoflavones phytoestrogen consumed in soya decrease. F. 2. -isoprostane concentrations and increase resistance of low-density lipoprotein to oxidation in humans. Am J Clin Nutr 2000; 72: 397-400.
[13] Guilarte TR, McGlothan JL. Selective decrease in NR1 subunit splice variant mRNA in the hippocampus of Pb2+-exposed rats: Implications for synaptic targeting and cell surface expression of NMDAR complexes. Brain Res Mol Brain Res 2003; 113: 37-43.
[14] Auclair A, Drouin C, Cotecchia S, Glowinski J, Tassin JP. 5-HT2A and alpha1b-adrenergic receptors entirely mediate dopamine release, locomotor response and behavioural sensitization to opiates and psychostimulants. Eur J Neurosci 2004; 20: 3073-84.
[15] Sinha AK. Colorimetric assay of catalase. Anal Biochem. 1972 Jun; 47(2): 389-94.
[16] Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, et al. Determination of carbonyl content in oxidatively modified proteins. Met Enzymol 1990; 186: 464-78.
[17] Niehaus WG, Samuelson B (1968) Formation of malondialdehyde from phospholipids arachidonate during microsomal lipid peroxidation. Eur J Biochem 6: 126-130.
[18] Millan M. The neurobiology and control of anxious states. Prog Neurobiol 2003; 70: 83-244.
[19] Cauli O, Morelli M. Subchronic caffeine administration sensitizes rats to the motor-activating effects of dopamine D1 and D2 receptor agonists. Psychopharmacology 2002; 162: 246-54.
[20] Becaria A, Bondy SC, Campbell A. (2003), Aluminium and copper interact in the promotion of oxidative but not inflammatory events: implications for Alzheimer’s disease. J Alzheimer Dis, 5, 31–8.
[21] Nehru B, Bhalla P, Garg A, (2007), Further evidence of centrophenoxine mediated protection in aluminium exposed rats by biochemical and light microscopy analysis. Food Chem Toxicol 45, 2499–505.
[22] Rafiq M, Thippeswamy A, Viswantha GLS, Kethaganahalli J. Kavya, Suryakanth D. Anturlikar, Pralhad S. Patki, (2013), Evaluation of Bacopa monniera for its Synergistic Activity with Rivastigmine in Reversing Aluminum. Induced Memory Loss and Learning Deficit in Rats. J Acupunct Meridian Stud. 6(4), 208-213.
[23] Ramachandran S, Sanjay AS, Dhanaraju MD, (2013), Antiamnesic effect of Piracetam potentiated with Emblica officinalis and Curcuma longain aluminium induced neurotoxicity of Alzheimer’s disease, Internation al Journal of Advanced Research, 1(7), 185-196.
[24] Gupta V, Anitha S, Hegde ML, Zecca L, Garruto RM, Ravid R, Shankar SK, Stein R, Shanmugavelu P, Jagannatha Rao KS: Aluminium in Alzheimer's disease: are we still at a crossroad?
[25] Christen Y (2000) Oxidative stress and Alzheimer disease. Am. J. Clin. Nutr 71: 6215-6295.
[26] Nehru, B. and Anand, P. (2005): Oxidative damage following chronic aluminium exposure in adult and pup rat brains. J. Tra. Elem. Med. Biol. 19: 203–208.
[27] Lin Y. L., Chang H. C., Chen T. L., Chang J. H., Chiu W. T., Lin J. W., et al. . (2010). Resveratrol protects against oxidized LDL-induced breakage of the blood-brain barrier by lessening disruption of tight junctions and apoptotic insults to mouse cerebrovascular endothelial cells. J. Nutr. 140, 2187–2192.
[28] Kumar V, Gill KD (2009). Aluminium neurotoxicity: Neurobehavioural and oxidative aspects. Arch. Toxicol. 83: 965–978.
[29] Germano C, Kinsella GJ, 2005, Working memory and learning in early Alzheimer’s disease. Neuropsychol Rev, 15, 1–10.
[30] Kaizer RR, Corrêa MC, Spanevello RM, Morsch VM, Mazzanti CM, Gonçalves JF, Schetinger MR, (2005), Acetylcholinesterase activation and enhanced lipid peroxidation after long-term exposure to low levels of aluminum on different mouse brain regions. J Inorg Biochem, 99, 1865–1870.
[31] Thorne, B.M., Donohoe, T., Lin, K.-N., Lyon, S., Medeiros, D. M. and Weaver, M.L.: Aluminium Ingestion and Behavior in the Long Evans Rat. In: Physiology & Behaviour. 1986, 36, p.63-67.
[32] Bouayed J, Rammal H, Younos C, Soulimani R. Positive correlation between peripheral blood granulocyte oxidative status and level of anxiety in mice. Eur J Pharmacol 2007; 564: 146–9.
[33] Kaviarasan S, Naik G, Gangabhagirathi R, Anuradha C, Priyadarsini K: In vitro studies on antiradical and antioxidant activities of fenugreek (Trigonella foenum graecum) seeds. Food Chemistry 2007, 103: 31-37.
[34] Kaviarasan S, Ramamurty N, Gunasekaran P, Varalakshmi E, Anuradha CV: Fenugreek (Trigonella foenum graecum) seed extract prevents ethanol-induced toxicity and apoptosis in Chang liver cells. Alcohol and alcoholism (Oxford, Oxfordshire) 2006, 41: 267-73.
[35] Skandhan KP, Rajahariprasad A: Estrogen in milk and plants. Medical Hypotheses 2005, 64: 429-430.
[36] Schreihofer DA: Phytoestrogens as neuroprotectants. Drugs of today (Barcelona, Spain: 1998) 2009, 45: 609-27.
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