International Journal of Plant Science and Ecology
Articles Information
International Journal of Plant Science and Ecology, Vol.2, No.2, Apr. 2016, Pub. Date: Jun. 1, 2016
Response of Promiscuous and Non-promiscuous Soybean (Glycine max (L) Merill) Cultivars to Indigenous Bradyrhizobium japonicum Inoculation in Three Ghanaian Soils
Pages: 15-22 Views: 2466 Downloads: 1810
Authors
[01] Klogo Phanuel Y., Agro Enterprise Development Department, Faculty of Applied Sciences and Technology, Ho Polytechnic, Ho, Ghana.
[02] Ofori Johnson K., Agricultural Engineering Department, Faculty of Engineering, Ho Polytechnic, Ho, Ghana.
[03] Glover Mawutor K., Agro Enterprise Development Department, Faculty of Applied Sciences and Technology, Ho Polytechnic, Ho, Ghana.
[04] Avumegah Patrick K., Agro Enterprise Development Department, Faculty of Applied Sciences and Technology, Ho Polytechnic, Ho, Ghana.
Abstract
Soybean, a recently introduced leguminous crop in West Africa, does not usually nodulate satisfactorily unless it is inoculated with selected rhizobial strains. The development of the Tropical Glycine Cross (TGx) soybean varieties by IITA has made it possible for this promiscuous varieties to nodulate with the naturally occurring strains with source belonging to the cowpea bradyrhizobia spp. For many tropical countries particularly in Africa, Biological Nitrogen Fixation (BNF) continues to be the most promising alternative or supplement to the use of chemical Nitrogen fertilizers for sustainable Agriculture. Greenhouse experiments were carried out at the University of Ghana-Legon to determine the effect inoculation of soybean with bradyrhizobia has on nodulation, nodule dry weight %N and total N in three promiscuous soybean varieties, Bengbie, TGx 1903-7E and TGx 1910-2F, and the cultivar Bragg, anon- promiscuous genotype in three Ghanaian soil series, Oyibi (Sodic solunchaus), Hake (Eutric cambisol) and Nzima (Ferric acrisols). The Most Probable Number (MPN) technique was used to determine the bradyrhizobial population in these soils. Eighty four Bradyrhizobium isolates obtained from randomly selected nodules were assessed for effectiveness in nitrogen fixation. Three of the most effective Bradyrhizobium isolates were used in the inoculation studies. There were tremendous inoculation responses in these soils for both the promiscuous and non-promiscuous cultivars, with even the promiscuous ones responding better to inoculation than the non-promiscuous Bragg. Inoculation gave rise to significant increases in nodule number, nodule dry weight, shoot dry weight and total nitrogen accumulation compared to the uninoculated control. However, the response of these soybean cultivars was higher in Hake soil series despite the high number of numerous indigenous bradyrhizobia strains in this soil which might have offset the inoculation response in this soil.
Keywords
Bradyrhizobium japonicum, Effectiveness, Inoculation, Non-promiscuous Soybean, Promiscuous Soybean
References
[01] Bell M. J., Wright, G. C., Suryantini, L., and Peoples, M. B., 1994. N2 fixing capacity of peanut cultivars with different assimilate partitioning characteristics, Australian Journal of Agricultural Research. 45: 1455-1468.
[02] Bremner, J. M., 1996. Nitrogen-Total. pp 1085-1121. In: methods of soil analysis. Chemical methods-SSSA Book series no 5. Soil Science Society of America and American Society of Agronomy. Madison Wisconsin, USA.
[03] Broughton. W. J., and Dilworth, M, 1970. N-free nutrient solution. In: Methods in Legume Rhizobium Technology.
[04] Chapman, H. D., 1965. Cation Exchange methods of soil analysis, part 2, chemical and microbial properties (Black, C. A., et, al., education), American Society of Agronomy, Inc Madison, Wisconsin, pp 8991-9000.
[05] Cuttelan A. J and Hungria, M., 1994. Nitrogen nutrition and inoculation. In: Tropical Soybean Improvement and Production; Brazillian Agric. Research Ent, FAO of the United Nations. Rome.
[06] Danso, S. K. A., 1992. Biological nitrogen fixation in tropical Agro-systems: twenty years of biological nitrogen fixation research in Africa. In Biological nitrogen fixation and sustainability of Tropical Agric. Pp 3-13. A wiley-sayce Co. Publication.
[07] Danso, S. K. A and Owiredu J. D., (1988a). Competitiveness of introduced and indigenous cowpea Bradyrhizobium strains, for nodule formation on cowpea [Vigna unguiculata (L) Walp] in three soils. Soil Biol and Bioc 20:305-310.
[08] Dashiell, K. E., Kueneman, E. A., Root W. R., and Singh S., 1983. Breeding tropical soybean for superior seed longevity and for nodulation with indigenous rhizobia. Pp 133-139. In: Shanmugasundaram, S and Sulzberger E. W (eds) Soybean in Tropical and Subtropical Cropping Systems. Fortune Printing Co. Ltd, Taiwan.
[09] Djagbletey, D., 1995. The nitrogen fixing potential of soybean bradyrhizobia from three Ghanaian Soils. Mphil Thesis. Univ. ofGhana-Legon.
[10] Fening, J. O. & Danso, S. K. A, 2002. Variation of symbiotic effectiveness of cowpeabradyrhizobia indigenous to Ghanaian soils. App Soil Ecol 21:23-29.
[11] Fening J O and Danso S K A, 2001. Response of cowpea to inoculation with indigenous Bradyrhizobium strains. Trop. Sci. 41, 172-176.
[12] Ferriera, E. M., and Marques, J. F., 1992. Selection of Portuguese Rhizobium legumiosarum bv. trifoli strains for production of legume inoculants. plant and soil 147: 151-158.
[13] Genstat., 2000. Genstat for windows, Release 4.14th ed. VSN International Ltd Oxford.
[14] Gyau, A. A., 2001. Nodulation promiscuity of soybean genotypes. Mphil. Thesis. Univ of Ghana- Legon.
[15] Isreal, D. W., 1993. Symbiotic dinitrogen fixation and host-plant growth during development of and recovery from phosphorus deficiency. Physiol. Plant. 88: 294-300.
[16] Kumaga, F. K, and Etu-Bonde, K., 2000. Response of two promiscuous soybean genotypes to bradyrhizobial inoculation in two Ghanaian soils. J. Ghana Association 2:99-104.
[17] Kumaga, F. K, and Ofori, 2004. Response of soybean [Glycine max (L) Merill] to bradyrhizobial inoculation and phosphorus application. Journalof Agric. And Biol. 2: 324-327
[18] Nangju, D., 1980. Soybean response to indigenous rhizobia as influenced by cultivar origin. Agron. J. 72: 403-406.
[19] Okereke, G. U., Onochie, C. C., Onukwo, E., and Ekejundu, G. O., 2000. Response of introduced Bradyrhizobium strains infecting a promiscuous soybean cultivar. World J. Microbiol. Biotech. 16:43-48.
[20] Pulver, E. L., Brockman, F., Wein, H. C., 1982. Nodulation of soybean cultivars with Rhizobiumspp and their response to inoculation with R. japonicum. Crop Sc. 22:1065-1070.
[21] Pulver, E. L., Kueneman, E. A., Ranga-Rao, V., 1985. Identification of promiscuous nodulating soybean. Efficient in N2- Fixation. Crop Sc. 25:660-663.
[22] Sanginga, N., Abaidoo, K., Danshiell, R. Carsky, J. Okogun, A., 1995. Persistence and effectiveness rhizobia nodulating promiscuous soybeans in moist savanna zones of Nigeria. Appl. Soil Ecol., 3:16-24.
[23] Sanginga, N., Thottapilly G., and Dashiell K., 1999. Effectiveness ofrhizobianodulating recent promiscuous soybean selectionin the moist savanna of Nigeria. Soil Biol. Biochem. 32:127-133.
[24] Singleton, P. W., Bohlool, B. B., Nakoo, P. L., 1992. Legume response to rhizobial inoculation in the tropics: myths andrealities. In myths and science of soils of the tropics. Soil Science Society of America. Special pub. 29:135-155.
[25] Somasegaran, P., and Hoben, H. J., 1985. Methods in Legume Rhizobium Technology. Hawaii Institute of Tropical Agric. And Human Resources. Univ. of Hawaii.
[26] Somasegaran P., and Hoben, H. J., 1994. Handbook for Rhizobium methods in Legume-Rhizobium Technology. Springer-Verlag, Heidelberg, Germany.
[27] Vincent, J. M., (1970). A manual for the Practical Study of Root-Nodule Bacterial I. B. P. Handbook No. 15. Blackwell Scientific Publications, Oxford.
[28] Woomer, P. W., Bennett, J., Yost, R., 1990. Overcoming the inflexibility of the most- probable-number procedures,Agron. Journal 82: 349-353.
600 ATLANTIC AVE, BOSTON,
MA 02210, USA
+001-6179630233
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - American Institute of Science except certain content provided by third parties.