[01] Ibtisam Mohammed Ali Alsudays, Department of Biology, Faculty of Applied Science, Umm Al Qura University, Makkah, Kingdom of Saudi Arabia; Department of Biology, Unaizah College of Sciences and Arts, Qassim University, Al Qassim, Kingdom of Saudi Arabia. [02] Hameda El Sayed Ahmed El Sayed, Department of Biology, Faculty of Applied Science, Umm Al Qura University, Makkah, Kingdom of Saudi Arabia.

The aimed of this studies to explain the role of Ascorbic acid (ASA), Gibberellic Acid (GA3) & Moringa oleifera Leaf Extract (MLE) for alleviates the adversely effect of salinity stress on two cultivars (cv. Cobra-resistant and cv. Newton-sensitive) of tomato (Lycopersicon esculentum, L.) plant which collected from Al-Dakhil Agriculture and Trading Establishment, Al-Qassim City, KSA. The tomato seeds soaked in in ASA 0.75 mM; GA3 0.05 mM and Moringa Extract MLE 5% before germinated for 12 hours in the dark. The seeds planted in trays of cork contain 218 eyes for 14 days, seedlings plant transplanted to plastic containers containing a mixture of sand/peat-moss (1:2). The tomato seeds watering using distilled water until the true leaf appearance then transferred to a larger pots which containing the same mixture of soil as above. The experiment was carried out under greenhouse conditions with temperature 18°C±1°C (night) & 22°C±2°C (day) and relative humidity varied between 60-70%. The tomato plant irrigated with NaCl salinity (0.0, 50, 100, 150, 200 mM NaCl) alternative with Hoagland nutrient solution. Salinity treatments decreased seeds germination rate (%), shoot height and root depth; fresh and dry weights; water relation (succulence) and all Photosynthetic pigments content (chlorophyll a, chlorophyll b, carotenoids, and total pigment contents) while the dry matter content (%) increased significantly with increasing NaCl salinity. Whereas the seeds soaking before germination in ASA, GA3 & MLE get an increased seeds germination rate (%), shoot height and root depth; fresh and dry weights; water relation (succulence and dry matter contents) and all Photosynthetic pigments content (chlorophyll a, chlorophyll b, carotenoids, and total pigment contents) compared with control. The results indicated that the plant growth parameters increased significantly in the present of ASA, GA3 & MLE under non-saline and salinity stress thereby reduces the harmful effects of salinity and increases resistance to salinity more than in the absent of ASA, GA & MLE compared with control. The application of soaking tomato seeds before germination in GA3, MLE, ASA respectively get evidence to the tolerance effect of salinity stress.

Growth Regulators, Ascorbic Acid, Gibberellic Acid, Moringa, Germination, Growth, Chloroplast Pigments, Tomato, Salinity Stress

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