International Journal of Plant Science and Ecology
Articles Information
International Journal of Plant Science and Ecology, Vol.1, No.3, Jun. 2015, Pub. Date: May 18, 2015
Evaluation of Antibacterial Potential of Stem Bark of Ethnomedicinal Tree Pittosporum dasycaulon Miq
Pages: 113-118 Views: 4527 Downloads: 964
Authors
[01] Bince Mani, Postgraduate and Research Department of Botany, St. Thomas College, Palai, Arunapuram (P.O.), Kottayam, Kerala, India.
[02] T. Dennis Thomas, Postgraduate and Research Department of Botany, St. Thomas College, Palai, Arunapuram (P.O.), Kottayam, Kerala, India.
Abstract
The ethnic people indigenous to Southern Western Ghats using stem bark of Pittosporum dasycaulon Miq. in their medicinal preparations to treat various skin diseases, leprosyand bronchitis and also used as antifungal and antibacterial agent. The dried stem bark of P. dasycaulon was serially extracted with petroleum ether, chloroform, acetone, methanol and water. The extracts were concentrated and their antibacterial activity was detected against thirteen bacterial strains by disc diffusion method. Minimum inhibitory concentration (MIC) of each extract was also determined. Chloroform and acetone extracts displayed a potential antibacterial activity, against all the tested gram-positive bacteria and eight gram-negative bacteria, compared to petroleum ether, methanol and aqueous extracts. Among the different fractions chloroform extract was most effective with maximum zone of inhibition (23.33±0.58 mm) and lowest MIC value (≤ 0.325 mg/ml) against Enterobacter aerogens. The aqueous extract was least effective against all the tested bacteria. The results of this investigation suggest that stem bark of Pittosporum dasycaulon can be used to discover broad spectrum antibacterial principles for developing new pharmaceuticals to control studied and many other human pathogenic bacteria responsible for severe illness.
Keywords
Pittosporum dasycaulon, Stem Bark, Ayurveda, Disc Diffusion, Minimum Inhibitory Concentration
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