Journal of Agricultural Science and Engineering
Articles Information
Journal of Agricultural Science and Engineering, Vol.2, No.5, Oct. 2016, Pub. Date: Nov. 2, 2016
Effect of Plant Hormone on Micropropagation and Callogenesis of Medicinal Plant (Curculigo orchioides)
Pages: 36-39 Views: 689 Downloads: 292
Authors
[01] Samaneh Zokae, Samaneh Zokae, MSC of Plant Pathology, College of Agriculture, Ferdowsi University of Mashhad, Iran.
[02] Tahmineh Esfandiyari Sabzevar, Tahmineh Esfandiyari Sabzevar, PhD Scholare of Medicenal Plant, College of Agriculture, Islamic Azad University of Shirvan, Iran.
[03] Raheleh Ahmadzadeh Ghavidel, Raheleh Ahmadzadeh Ghavidel, Phd of Agroecology, College of Agriculture, Ferdowsi University of Mashhad, Iran.
Abstract
Curculigo orchioides is a perennial herb of the family Hypoxidaceae. It is an endangered plant species of medicinal importance. In this study a simple and efficient protocol for in vitro micropropagation of Curculigo orchioides. Through shoot tip and nodal portion explants and callogenesis of Curculigo orchioides through leaf, node and explants was used. A completely randomized design was followed for the experiment multiple shoots were obtained on all concentrations of BAP and NAA, but BAP (1 mg/l) showed the best response (90 and 80%) from both explants. Similarly, the best callus response was observed on MS medium supplemented with 2, 4- D + Kin (1+1 mg/l) in all explants. Then plantlets were transferred to different potting media in green house for acclimatization at the end of a three-month period, an average of 125 plants were obtained from a single meristem.
Keywords
Curculigo orchioides, Micropropagaton, Plant hormones, Tissue Culture
References
[01] Faheem, M., S. Singh, B. S. Tanwer, M. Khan and A. Shahzad. (2011). In vitro regeneration of multiplication shoots in Catharanthus roseus- An important medicinal plant. Adv. Appl. Sci. Res. 2 (1): 208-213.
[02] Jasrai, Y. T., Wala, B. B., (2000). Curculigo orchioides Gaertn. (Kali musli): An endangered medicinal herb, In: Role of Biotechnology in Medicinal and Aromatic Plants, Vol. IV, Khan IA and Khanum A (Eds.), Ukaaz Publications, Hyderabad, India, pp. 89-95.
[03] Kirtikar, K. R., Basu, B. D., (1935). Indian Medicinal Plants, Vol. IV, Blatter E, Caius JF and Mhaekar KS (Eds.), Bishen Singh Mahendra Pal Singh, Delhi, India, pp. 2469-2470.
[04] Murashige, T., F. Skoog. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
[05] Murch, S. J., R. S., Krishna, P. K., Saxena. (2000). Tryptophan as a precursor for melatonin and serotonin biosynthesis in in-vitro regenerated St. John's ort (Hypericum perforatum. cv. Anthos) plants. Plant Cell Rep. 19: 698-704.
[06] Nadgauda, R. S., Mascarenhas, A. F., Hendre, R. A., Jagannathan, V. (1978). Rapid miltiplication of turmeric (Curcuma longa L.). Indian J. Exp. Biol., 16: 120-122.
[07] Narayanswamy, S., (1997). Plant Cell and Tissue Culture, Tata McGraw-Hill Publishing Co. Ltd., New Delhi, India, pp. 116-118.
[08] Purohit, S. D, Dave, A., Gotam, K. (1994). Micropropagation of safed musli (Chlorophytum borivilianum), a rare Indian medicinal herb. Plant Cell Tiss. Org. Cult., 39: 93-96.
[09] Ramawat, K. G., Sonie, K. C., Sharma, M. C. (2004). Therapeutic potential of medicinal plants: An introduction. In: Biotechnology of Medicinal Plants: Vitalizer and Therapeutics (Ramawat, K. G. Eds.), Sci. Pub. Inc., Enfield (NH), USA, p 1-18.
[10] Rolf, D. I., Ricardo, T. F. (1995). Micropropagation of Alpinia purpurata from inflorescence buds. Plant Cell Tiss. Org. Cult., 40: 183-185.
[11] Rout, G. R., Samantray, S., Das, P. (2000). In vitro manipulation and propagation of medicinal plants. Biotechnology Advances 18: 91-120.
[12] Sidhu, Y. (2010). In vitro micropropagation of medicinal plants by tissue culture. The Plymouth Student Scientist. 4 (1): 432-449.
600 ATLANTIC AVE, BOSTON,
MA 02210, USA
+001-6179630233
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - 2017 American Institute of Science except certain content provided by third parties.