Journal of Nanoscience and Nanoengineering
Articles Information
Journal of Nanoscience and Nanoengineering, Vol.1, No.4, Dec. 2015, Pub. Date: Dec. 6, 2015
Biosynthesis of Silver Nanoparticles Using an Endophytic Fungus, Curvularialunata and Its Antimicrobial Potential
Pages: 241-247 Views: 1433 Downloads: 1245
[01] Parthasarathy Ramalingmam, Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, TamilNadu, India.
[02] Sathiyabama Muthukrishnan, Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, TamilNadu, India.
[03] Prabha Thangaraj, Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, TamilNadu, India.
The development of reliable green process for the synthesis of silver nanoparticles is an important branch of Nanobiotechnology. In the present investigation the use of the endophytic fungus Curvularialunata for the extracellular biosynthesis of silver nanoparticles (AgNPs) from silver nitrate solution is reported. It was observed that the aqueous silver (Ag+) ions, when exposed to a filtrate of C. lunata, were reduced in solution, resulting in the formation of stable AgNPs. These AgNPs were characterized by means of several techniques. The nanoparticles show maximum absorbance at 422 nm on ultraviolet-visible spectra. The presence of protein was identified by Fourier Transform infrared spectroscopy. The reduction of Ag+ ion to elemental silver was characterized by Energy - dispersive X-ray (EDX) Spectroscopy. Scanningelectron micrograph revealed the formation of polydispersed nanoparticles of 10-50 nm. The nanoparticles were also evaluated for their enhanced antimicrobial activities with various antibiotics against gram positive and gram negative bacteria. The antibacterial activities of Ampicillin, Rifampicin, Chloramphenicol, Erythromycin, and Kanamycin were increased in the presence of AgNPs against test strain. The highest fold increase of area was found for Erythromycin and Carbenicilin against E. coli, Ampicilin against S. paratyphi, Erythromycin against B. subtillis. The results showed that the combination of antibiotics with AgNPshas better antimicrobial effects.
Endophytic Fungi, Silver Nanoparticles, SEM, FTIR, Antibacterial Activity
[01] D. Sindhupriya, M. Amitava, N.Chandrasekaran. Synergistic effect of biogenic silver nanocolloid in combination with antibiotics: a potent therapeutic agent, International Journal of Pharmacy and Pharmaceutical Sciences.5, 0975-1491, 2013.
[02] P. Ahmad, P. Mukherjee, S. Senapati, D. Mandal, M.I. Khan, M.Sastry. Extracellular biosynthesis of silver nanoparticles using the fungus Fusariumoxysporum. Colloids Surface B Biointerfaces. 28, 313–318, 2003.
[03] S. Anusuya, M. Sathiyabama. Preparation of β-D-glucan nanoparticles and its antifungal activity, Int J BiolMacromol. 70, 440-3, 2014.
[04] R.M. Slawson, J.T. Trevors, H. Lee. Silver accumulationand resistance in Pseudomonas stutzeri. Arch Microbiol. 158, 398-404, 1992.
[05] N. Ahmad, S. Sharma, V.N. Singh, S.F. Shamsi, A. Fatima, B.R. Mehta. Biosynthesis of silver nanoparticles from Desmodiumtriflorum: A novel approach towards weed utilization. Biotechnol Res Int.1-8, 2011.
[06] G. Sathishkumar, C. Gobinath, K. Karpagam V. Hemamalini K. Premkumar, S. Sivaramakrishnan.Phyto-synthesis of silver nano scale particles using Morindacitrifolia L. and its inhibitory activity against human pathogens. Coll Surf B 95: 235–240, 2012.
[07] J. Antony, P. Sivalingam, D. Siva, S. Kamalakkannan, K. Anbarasu, R. Sukirtha, M. Krishnan, S. Achiraman,. Comparative evaluation of antibacterial activity of silver nanoparticles Synthesized using Rhizophoraapiculata and glucose. Colloids Surface B Biointerfaces.88, 134-140, 2011.
[08] G. Strobel, B. Daisy. Bioprospecting for Microbial Endophytes and Their Natural Products. Microbiology and Molecular Biology Reviews. 67(4), 491-502, 2003.
[09] R. Parthasarathy, M. Sathiyabama. Gymnemagenin-producing endophytic fungus isolated from a medicinal plant Gymnemasylvestre R.Br. ApplBiochemBiotechnol. 172: 3, 141–3152, 2014.
[10] R. Parthasarathy, M. Sathiyabama. Lovastatin-producing endophytic fungus isolated from a medicinal plant Solanumxanthocarpum. Natural Product Research. 14786419.2015-1016938, 2015.
[11] V.C. Verma, R.N. Kharwar, A.C. Gange, Biosynthesis of antimicrobial silver nanoparticles by the endophytic fungus Aspergillusclavatus. Nanomedicine: Nanotechnology. Biology and Medicine. 5, 33–40, 2010.
[12] Y. Qian, H. Yu, D. He, H. Yang, W.Wang, X. Wan, L. Wang. Biosynthesis of silver nanoparticles by the endophytic fungus Epicoccumnigrum and their activity against pathogenic fungi. Bioprocess Biosyst. Eng, 36, 1613-1619, 2013.
[13] S, Yang, T. Chen. K. Li T. Tsai. Change in phenolic compound content, reductive capacity and ACE inhibitory activity in noni juiceduring traditional fermentation. J. Food Drug Anal. 15 (3), 290-298, 2007.
[14] M.Y. Wang, C. Su. Cancer preventive effect of Morindacitrifolia (Noni). Ann N Y Acad Sci. 952, 161-8, 2001.
[15] P. Mulvaney. Surface plasmon spectroscopy of nanosized metal nanosized metal particles. Langmuir, 12, 788–800, 1996.
[16] M.R. Eftink, C.A. Ghiron, Fluorescence quenching studies with proteins. Anal. Biochem. 114, 199-227, 1981.
[17] S. He, Z. Guo, Y.Zhang, S. Zhang, J. Wang, N. Gu, Biosynthesis of gold nanoparticles using the bacteria Rhodopseudomonas capsulate, Materials Latter. 61, 3984-3987, 2007.
[18] C.J.R Klittich, J.F Leslie. Nitrate reduction mutants of Fusariummoniliforme (gibberella-fujikuroi) Genetics. 118,417–423. 1988.
[19] J.R Lloyd. Microbial metal reduction. FEMS Microbiol. Rev. 27, 411, 2003.
[20] A. Ahmad, P.Mukherjee, S. Senapati, D. Mandal, M.I. Khan, M. astry. Extracellular biosynthesis of silver nanoparticles using the fungus Fusariumoxysporum. Colloids Surface B Biointerfaces. 28, 313–318. 2003.
[21] P.K. Jain, X.H. Hunag, I.H. EI-Sayed, M.A. EI-Sayed. Noble metals on the nanoscale: optical and phtothermal properties and some applications in imaging, sensing, biology and medicine. Acc. Chem. Res.41, 1578-1586, 2008.
[22] A. Manikandan, M. Sathiyabama. Green Synthesis of Copper-Chitosan Nanoparticles and Study of its Antibacterial Activity. J NanomedNanotechnol. 157-7439, 2015.
[23] P. Magudapathy, P. Gangopadhyay, B.K. Panigrahi, K.G.M. Nair, S. Dhara. Ion beam sputtering and nanostructures of noble metals. Physics B. 299, 142-146, 2001.
[24] J.R. Morones, Jose L. Elechiguerra A. Camacho, HoltK, J.B. Kouri J. Tapia Raḿırez M. Jose Yacaman. The bactericidal effect of silver nanoparticles. Nanotechnology 16, 2346–2353, 2005.
[25] Q.L. Feng, J. Wu, G.Q. Chen, F.Z. Cui, T.N. Kim, J.O. Kim, "A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus." Journal of Biomedical Materials Research Part A. Volume 52, issue 4, P, 662-668, 2000.
MA 02210, USA
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - 2017 American Institute of Science except certain content provided by third parties.