[01] P. Kalainila, Department of Biotechnology, A. C. Tech. Campus, Anna University, Chennai, Tamilnadu, India. [02] R. S. Ernest Ravindran, Department of Biotechnology, A. C. Tech. Campus, Anna University, Chennai, Tamilnadu, India. [03] R. Rohit, Department of Applied Science and Technology, A. C. Tech. Campus, Anna University, Chennai, Tamilnadu, India. [04] S. Renganathan, Department of Biotechnology, A. C. Tech. Campus, Anna University, Chennai, Tamilnadu, India.

A green method of synthesizing silver nanoparticles (AgNPs) using Kigelia Africana leaves extract as reducing and stabilizing agent. The formation and stability of the reduced silver nanoparticles in the colloidal solution were monitored by UV–visible spectrophotometer analysis at 445 nm. FTIR spectrum showed the functional groups that were present in the leaf extract were responsible for the reduction of AgNPs. XRD result shows that the particles are in crystalline nature and size ranging from 70 to 140 nm with spherical structure from SEM analysis. In addition, the characterized AgNPs has the potential antimicrobial effect against various pathogens such as Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The zone of inhibition of AgNPs was observed to be high for all pathogens.

AgNPs, Antimicrobial Study, Bioactive Molecules, Bio-reduction, Kigelia Africana

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