American Journal of Food Science and Health
Articles Information
American Journal of Food Science and Health, Vol.2, No.4, Aug. 2016, Pub. Date: Jun. 28, 2016
Prevalence of Listeria monocytogenes in Beef, Chevon and Chicken in Bangladesh
Pages: 39-44 Views: 492 Downloads: 423
Authors
[01] M. S. Islam, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
[02] A. A. Husna, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
[03] M. A. Islam, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
[04] M. M. Khatun, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Abstract
The present research was conducted for the isolation and characterization of the Listeria monocytogenes from meat samples collected from different local markets around Mymensingh town such as Boira, Paglar bazar, Kawatkhali bazar, Kamal-Ranjit (K-R) market and Shes More bazar. A total of 36 meat samples were collected from cattle, goat and chicken. After preparation the samples were inoculated into different selective media such as Oxford Agar, Mannitol Salt Agar and Blood Agar for isolation and identification of L. monocytogenes. In Oxford Agar, Listeria spp. produced black zone around the colonies. Gram staining, motility test, blood hemolysis, Christe Atkins Munch Peterson (CAMP) test and biochemical test were performed to confirm L. monocytogenes. The L. monocytogenes fermented dextrose and maltose with the production of only acid and no gas was observed in Durham′s tube. The L. monocytogenes were found Methyl-Red and Voges-Proskauer (MR-VP) test positive but indole negative. L. monocytogenes were catalase, CAMP test positive and motile. Among 36 meat samples, 4 (11.11%) were L. monocytogenes. Among animal species, the distribution of L. monocytogenes was 8.33% (1/12) in chicken, 16.66% (2/12) in beef and 8.33% (1/12) in chevon. The market wise distribution of L. monocytogenes was 0% in Boira, Paglar bazar, 11.11% in KR market, 22.22% in Shesmore bazar and 11.11% in Kawatkhali bazar. The L. monocytogenes isolate was resistant to ampicillin and penicillin; sensitive to the ciprofloxacin, vancomycin and gentamicin. This is the first report on isolation and identification of L. Monocytogenes from beef, chevon and chicken in Bangladesh. The multidrug resistant L. monocytogenes may be transmitted to the human through consumption of contaminated meat and may lead to public health hazard.
Keywords
Beef, Chevon, Chicken, Listeria, Meat, Multidrug-Resistant
References
[01] Indrawattana N, Nibaddhasobon T, Sookrung N, Chongsa-nguan M, Tungtrongchitr A, Makino S, Tungyong W and Chaicumpa W (2011). Prevalence of Listeria monocytogenes in raw meats marketed in Bangkok and characterization of the isolates by phenotypic and molecular methods. Journal of Health, Population and Nutrition, 29: 26-38.
[02] EFSA (European Food Safety Authority (2012). Trends and sources of zoonoses, zoonotic agents and antimicrobial resistance in the European Union in 2010. EFSA Journal. pp. 2597.
[03] Wu S, Wu Q, Zhang J, Chen M, Yan Z, Hu H (2015). Listeria monocytogenes prevalence and characteristics in retail raw foods in China. PLoS ONE 10(8): e0136682. doi:10.1371/journal.
[04] Torres KJ, Sierra SC, Poutou RA, Carrascal AK, Mercado M (2005). Patogénesis of Listeria monocytogenes, microorganism zoonotic emergent. Revista MVZ Córdoba, 10: 511-543.
[05] Farber JM, Peterkin PI (1991). Listeria monocytogenes, a foodborne pathogen. Research Journal of Microbiology, 55: 476-511.
[06] Lyytikäinen O, Nakari UM, Lukinmaa S, Kela E, Nguyen TMN, Siitonen A (2006). Surveillance of listeriosis in Finland during 1995-2004. European Surveillance, 11: 82-85.
[07] Kvistholm J (2010). Substantial increase in listeriosis, Denmark. European Surveillance, 15: 1-4.
[08] Guillet C, Lambert JO, Le Monnier A, Leclercq A, Mechaï F, Bruneel MMF, Bielecka MK, Scortti M, Disson O, Berche P, Boland VJ, Lortholary O, Lecuit M (2010). Human listeriosis caused by Listeria ivanovii. Emerging Infectious Disease, 16: 136-138.
[09] Dhama K, Verma AK, Rajagunalan S, Kumar A, Tiwari R, Chakraborty S, Kumar R (2013). Listeria monocytogenes infection in poultry and its public health importance with special reference to food borne zoonoses. Pakistan Journal of Biological Science, 16: 301-308.
[10] Adzitey F, Huda N (2010). Listeria monocytogenes in foods incidence and possible control measures. African Journal of Microbiological Research, 25: 2848-2855.
[11] Barbuddhe S, Malik SVS, Bhilegaonkar KN, Kumar P, Gupat LK(2000). Isolation of Listeria monocytogenes and anti-listeriolysin O detection in sheep and goats. Small Ruminant Research, 38: 151-155.
[12] Çolak H, Hampikyan H, Bingöl BE, Ulusoy B (2007). Prevalence of Listeria monocytogenes and Salmonella spp. in Tulum cheese. Food Control, 18: 576-579.
[13] Elmalı M, Oner S, Yaman H (2013). Detection of Listeria monocytogenes by using PCR method in refrigerated ready to eat food consumed in Turkey. Medycyna Weterynaryjna, 69: 36-39.
[14] Şireli UT,Gücükoğlu A. (2008). Prevalence and antibiotic resistance of Listeria spp. isolated from ready-to-eat foods in Ankara. Turkish Journal of Veterinary and Animal Sciences, 32: 131-135
[15] Erol I (2007). Listeria. In I. Erol (Ed.), Food hygiene and microbiology (pp. 126-134). Ankara, Turkey: Pozitif Matbaacılık.
[16] Kathariou S (2002). Listeria monocytogenes virulence and pathogenicity, a food safety perspective. Journal of Food Protection, 65: 1811-1829.
[17] Deb R, Kumar A, Chakraborty S, Verma AK, Tiwari R, Dhama K, Singh U, Kumar S (2013). Trends in diagnosis and control of bovine mastitis: A review. Pakistan Journal of Biological Science, 16: 1653-1661.
[18] Charpentier E, Courvalin P (1999). Antibiotic resistance in Listeria spp. International Journal of Food Microbiology, 43: 2103-2108.
[19] Conter M, Paludi D, Zanardi E, Ghidini S, Vergara A (2009). Characterization of antimicrobial resistance of foodborne Listeria monocytogenes. International Journal of Food Microbiology, 128: 497-500.
[20] Pesavento G, Ducci B, Nieri D, Comodo N and Lo Nostro A (2010). Prevalence and antibiotic susceptibility of Listeria spp. isolated from raw meat and retail foods. Food Control, 21: 708-713.
[21] Shen Y, Liu Y, Zhang Y, Cripe J, Conway W (2006). Isolation and characterization of Listeria monocytogenes isolates from ready-to-eat foods in Florida. Applied and Environmental Microbiology, 72: 5073-5076.
[22] Korsak D, Borek A, DanilukS, Grabowska A, Pappelbaum K (2012). Antimicrobial susceptibilities of Listeria monocytogenes strains isolated from food and food processing environment in Poland. International Journal of Food Microbiology, 158: 203-208.
[23] Cowan ST (1985). Cowan and Steel’s Manual for Identification of Bacteria. 2nd edn. Of Listeriosis in France. American Journal of Epidemiology, 154: 944-950.
[24] Bauer AW, Kirdy WMN, Sheris JC, Truck M (1966). Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology, 145: 225-230.
[25] Clinical and Laboratory Standards Institute (CLSI, formerly NCCLS) (2007). Performance standards for antimicrobial susceptibility testing. 17th informational supplement document M100-S17:1. Wayne, Pennsylvania.pp. 32-50.
[26] Uyttendaele M, Troy PD, Debevere J (1999). Incidence of Listeria monocytogenes in different types of meat products on the Belgian retail market. International Journal of Food Microbiology, 53: 75-80.
[27] Farber JM (2000). Present situation in Canada regarding Listeria monocytogene sand ready-to-eat sea food products. International Journal of Food Microbiology, 62: 247-251.
[28] Vitas AI, Aguadoe V, Garcia-Jalon I (2004). Occurrence of Listeria monocytogenes in fresh and processed foods in Navarra (Spain). International Journal of Food Microbiology,90: 349-356
[29] Merchant IA, Packer RA (1967). Veterinary Bacteriology and Virology. 7th edition. The Iowa University Press, Ames, Iowa, USA. pp. 211-306; 387.
[30] Cheesbrough M (1985). Medical laboratory manual for tropical countries. 1st edition, Microbiology. English Language Book Society, London. pp. 400-480.
[31] Liu D (2008). Epidemiology: In Handbook of Listeria monocytogenes. CRC press, Boca Raton. pp. 27-60.
[32] Carpentier B, Cerf O (2011). Review-persistence of Listeria monocytogenes in food industry equipment and premises. International Journal of Food Microbiology, 145: 1-8.
[33] CDC (2012). Multistate outbreak of listeriosis linked to whole cantaloupes from Jensen Farms, Colorado. Available at: http://www.cdc.gov/listeria/outbreaks
[34] Beak SY, Lim YS, Lee DH, Min KH, Kim CM (2000). Incidence and characterization of Listeria monocytogenes from domestic and imported foods in Korea. Journal of Food Protection,63: 186-189
[35] Hudson JA, Mott SJ, Delacy KM, Edridge AL (1992). Incidence and coincidence of Listeria spp. motile aero monads and Yersinia enterocolitica on ready-to-eat flesh foods. International Journal of Food Microbiology, 16: 99-108.
[36] Cheroutre-Vialette M, Lebert I, Hebraud M, Lebert LA (1998). Effects of pH or aw stress on growth of Listeria monocytogenes. International Journal of Food Microbiology, 42: 71-77.
[37] Vermeulen A, Gysemans KPM, Bernaets K, Geeraerd Ah, Van Impe JF, DebevereJ, Devlieghere F (2007). Influence of pH, water activity and acetic acid concentration on Listeria monocytogenes at 7°C: data collection for the development of a growth/no growth model. International Journal of Food Microbiology, 114: 332-341.
[38] Zhang Y, Yeh E, Hall G, Cripe J, Bhagwat AA, Meng J (2007). Characterization of Listeria monocytogenes isolated from retail foods. International Journal of Food Microbiology, 113: 47-53.
[39] George F, Anders J, Joachim F, Vincent P (2009). Prevalence, genetic diversity and antimicrobial susceptibility of Listeria monocytogenes isolated from open-air food markets in Greece. Food Control, 20: 314-327.
[40] Wong H, Chao W, Lee S (1990). Incidence and characterization of Listeria monocytogenes in foods available in Taiwan. Applied and Environmental Microbiology, 56: 3101-3104.
[41] Karakolev R (2009). Incidence of Listeria monocytogenes in beef, pork, raw-dried and raw-smoked sausages in Bulgaria. Food Control, 20: 953-955.
[42] Altuntas EG (2012). Antibiotic and bacitracin sensitivity of Listeria monocytogenes strains isolated from different foods. Food Nutrition and Science, 3: 363-368.
[43] Soni DK, Singh RK, Singh DV, Dubey SK (2013). Characterization of Listeria monocytogenes isolated from ganges water, human clinical and milk samples at Varanasi, India. International Journal of Microbial Resource Technology, 14: 83-91.
[44] Palumbo D, Iannaccone M, Porta A, Capparelli R (2010). Experimental antibacterial therapy with puroindolines, lactoferrin and lysozyme in Listeria monocytogenes infected mice. Microbial Infection, 12: 538-545.
[45] Yan H, Neogi SB, Mo Z, Guan W, Shen Z, Zhang S, Li L, Yamasaki S, Shi L, Zhong N (2010). Prevalence and characterization of antimicrobial resistance of foodborne Listeria monocytogenes isolates in Hebei province of Northern China, 2005-2007. International Journal of Food Microbiology, 144: 310-316.
[46] Teuber M (1999). Spread of antibiotic resistance with food-borne pathogens. Cellular and Molecular Life Science,56:755-763.
[47] Rota C, Yang UJ, Blanco D, Carraminana J, Arino J, Herrera A (1996). High prevalence of multiple resistance to antibiotics in 144 Listeria isolates from Spanish dairy and meat products. Journal of Food Protection, 59: 938-943.
[48] Poyart-SalmeroC, Carlier C, Trieu-Cuot P, Courtieu AL, Courvalin P (1990). Transferable plasmid-mediated antibiotic resistance in Listeria monocytogenes. Lancet, 335: 1422-1426.
[49] Roberts MC, Facinelli B, Giovanetti E, Varaldo PE (1996). Transferable erythromycin resistance in Listeria spp. isolated from food. Applied and Environmental Microbiology, 62: 269-270.
[50] Facinelli B, Giovanetti E, Varaldo PE, Casolari P, Fabio U (1991). Antibiotic resistance in foodborne Listeria spp. Lancet, 3: 1268-1272.
600 ATLANTIC AVE, BOSTON,
MA 02210, USA
+001-6179630233
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - 2017 American Institute of Science except certain content provided by third parties.