American Journal of Food Science and Health
Articles Information
American Journal of Food Science and Health, Vol.2, No.4, Aug. 2016, Pub. Date: Jul. 15, 2016
Antioxidant Activity, Total Phenol Content, and Bioactive Components of Lindur Leave (Bruguierra gymnorrhiza)
Pages: 65-70 Views: 3839 Downloads: 1283
Authors
[01] Nurjanah Nurjanah, Department of Aquatic Product Technology, Faculty of Fisheries and Marine Science Bogor, Agricultural University Agatis Street Darmaga Bogor, West Java, Indonesia.
[02] Agoes M. Jacoeb, Department of Aquatic Product Technology, Faculty of Fisheries and Marine Science Bogor, Agricultural University Agatis Street Darmaga Bogor, West Java, Indonesia.
[03] Taufik Hidayat, Department of Aquatic Product Technology, Faculty of Fisheries and Marine Science Bogor, Agricultural University Agatis Street Darmaga Bogor, West Java, Indonesia.
[04] Siti Hazar, Department of Aquatic Product Technology, Faculty of Fisheries and Marine Science Bogor, Agricultural University Agatis Street Darmaga Bogor, West Java, Indonesia.
[05] Roni Nugraha, Department of Aquatic Product Technology, Faculty of Fisheries and Marine Science Bogor, Agricultural University Agatis Street Darmaga Bogor, West Java, Indonesia.
Abstract
Lindur are mangrove plants that has been widely used as drug, yet informations on potential drug compounds and antioxidants contained in lindur were still limited. This study were determined to determine the chemical composition, bioactive components, antioxidant activity, and total phenol contained in lindur. The result showed that lindur fresh leaves had a moisture content, ash, fat, protein, and crude fiber of 54.91%, 4.49%, 1.87%, 4.45%, and 8.67%; respectively. Lindur dried leaves had water, ash, fat, protein, and crude fiber content of 9.79%, 8.19%, 3.90%, 9.78%; and 18.05%, respectively. The methanol extract had the highest yield (15,79%) compared to the ethyl acetate extract (2.85%), and n-hexane (1.53%). Phytochemical analysis showed the bioactive components of n-hexane extract (phenol steroids and hydroquinone); ethyl acetate extract (steroids, hydroquinone phenols, flavonoids, saponins) and methanol extract (steroids, hydroquinone phenols, flavonoids, tannins, saponins). Methanol extract showed the highest antioxidant activity with IC50 values of 81.11 ppm, while the n-hexane extract showed the lowest activity with IC50 values of 815.67 ppm. Total phenolic extracts of n-hexane, ethyl acetate, and methanol, were 12.41, 97.57, and 30.07 mg GAE/ g extract, respectively.
Keywords
Antioxidant, Bioactive, Bruguiera gymnorrhiza, Leave, Phenol
References
[01] Allen JA, Duke NC. 2006. Bruguiera gymnorrhiza (large-leaf mangrove). www.traditionaltree.com [9 Juli 2013].
[02] Agoramoorthy G, Chen F, Venkatesalu V, Kuo DH, Shea, PC. 2008. Evaluation of antioxidant polyphenols from selected mangrove plants of India. Asian Journal of Chemistry 20(2):1311-1322.
[03] Andayani R, Y Lisawati, Maimunah. 2008. Penentuan aktivitas antioksidan, kadar phenol total, dan likopen pada buah tomat (Solanum lycupersicum L). Jurnal Sains dan Teknologi Farmasi 13(1): 1-9.
[04] Aranda SR, Perez-Lopez LA, Arroyo JL, Alanis-Garza BA, de Torres NW. 2009. Antimicrobial and antioxidant activities of plants from Northeast of Mexico. Journal of Evidence-Based Complementary and Alternative Medicine 41(5): 233-236.
[05] Darusman LK, Sajuthi D, Sutriah K, Pamungkas D. 1995. Ekstraksi komponen bioaktif sebagai bahan obat dari karang-karangan, bunga karang dan ganggang di perairan Pulau Pari Kepulauan Seribu [laporan penelitian]. Bogor: Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor.
[06] Duenas M, Manzano SO, Paramas AG, Buelga SC. 2009, Antioxidant evaluation of O-methylated metabolites of catechins, epicatechin, and quersetin. Journal of Pharmaceutical and Biomedical Analysis.
[07] Duke NC, James AA. 2006. Bruguiera gymnorrhiza (large-leafed mangrove). Species Profiles for Pacific Island Agroforestry Apr; Ver 2.I. www.traditionaltree.org [10 Oktober 2013].
[08] Haq M, Wirakarnain S, ABMS Hossain, Rosrna Mat Taha, KM Monneruzzaman. 2011. Total phenolic contents, antioxidant and antimicrobial activities of Bruguiera gymnorrhiza 6(17): 4112-4118.
[09] Harborne JB. 1987. Metode Fitokimia. Edisi ke-2. Padmawinata K, Soediro I, penerjemah. Bandung: Institut Teknologi Bandung. Terjemahan dari: Phytochemical Methods.
[10] Homhual S, Bunyapraphatsara N, Kondratyuk T, Herunsalee A, Chaukul W, Puzzuto JM, Fong HHS, Zhang HJ. 2006. Bioactive dammarane triterpenes from the mangrove plant Bruguiera gymnorrhiza. Journal of Natural Product 69 (3): 421-424.
[11] Ismail J, Max RJM, Feti F. 2012. Penentuan total phenolik dan uji aktivitas antioksidan pada biji dan kulit buah pinang yaki (Areca vestiaria Giseke). Jurnal Ilmiah Sains 12(2): 84-88.
[12] Kusnandar F. 2010. Kimia Pangan. Jakarta (ID): PT Dian Rakyat.
[13] Langseth L. 1995. Oxidants, Antioxidants and Disease Prevention. Belgium: ILSI Europe.
[14] Middleton EC, Kandaswami, TC Theoharides. 2000. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacological Reviews 52: 673-751.
[15] Muchtadi D. 1993. Aspek Biokimia dan Gizi dalam Keamanan Pangan. Bogor (ID): Pusat Antar Universitas Pangan dan Gizi, IPB Pr.
[16] Muchtadi TR dan Fitriyono A. 2010. Teknologi Proses Pengolahan Pangan. Bandung (ID): PT Alfabeta.
[17] Noor YR, Khazali M, Suryadiputra INN. 2006. Panduan Pengenalan Mangrove di Indonesia. Wetlands International-Indonesia Programme. Bogor: Ditjen PHKA.
[18] Premanathan M, Arakaki R, Izumi H, Kathiresan K, Nakano M, Yamamoto N, Nakashima H. 1999. Antiviral properties of a mangrove plant, Rhizophora apiculata Blume, against human immunodeficiency virus. Antiviral Res. 44(2): 113-22.
[19] Reynertson KA. 2007. Phytochemical Analysis of Bioactive Constituens from Edible Myrtaceae Fruit. [Dissertation]. The City University of New York.
[20] Salamah E, Ayuningrat E, Purwaningsih S. 2008. Penapisan awal komponen bioaktif dari kijing taiwan (Anadonta woodiana Lea.) sebagai senyawa antioksidan. Buletin Teknologi Hasil Perikanan 11(2): 119-132.
[21] Ukieyanna E. 2012. Aktivitas antioksidan, kadar phenolik, dan flavonoid tumbuhan suruhan (Peperomia pellucida L. Kunth) [skripsi]. Bogor: Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor.
[22] Wibowo C, Kusmana C, Suryani A, Hartati Y, Oktadiyani P. 2009. Pemanfaatan pohon mangrove api-api (Avicennia spp.) sebagai bahan pangan dan obat. Prosiding Seminar Hasil-Hasil Penelitian IPB 2009 Buku 1: bidang pangan dan energi. Bogor: LPPM-IPB.
[23] Wichi HP. 1988. Enhanced tumour development by butylated hydroxytoluene (BHT) from the properties of effect on fure stomach and esophageal aquamoua epithelium. Food Chemical Toxicology 26: 723-727.
[24] Winarsi H. 2007. Antioksidan Alami dan Radikal Bebas. Yogyakarta (ID): Kanisius.
[25] Yunizal, Murtini JT, Dolaria N, Purdiwoto B, Abdulrokhim, Carkipan. 1998. Prosedur Analisis Kimiawi Ikan dan Produk Olahan Hasil-Hasil Perikanan. Jakarta (ID): Pusat Penelitian dan Pengembangan Perikanan.
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